Abstract: Quantitative description of ecosystem services (ES) interactions is of great significance for rational allocation of resources and sustainable development in the new urban areas. In this study multiple ESs were calculated, including carbon storage (CS), habitat quality (HQ) and water yield (WY) in Nanjing Jiangbei New Area. The ESs' tradeoff-synergy relationships were assessed at different scales using InVEST model, GIS technology and the land use/land cover data respectively in 2000, 2010 and 2020. The results show that: (1) From 2000 to 2020, in Nanjing Jiangbei New Area, the HQ and CS decreased from 0.490 5 and 2.502×10⁷ t to 0.461 3 and 2.460×10⁷ t, respectively. The high values of CS and HQ were mainly distributed in forestland, while the low values were located in urban construction land such as Luhe Development Zone. The WY increased by 6.65×10⁸ m³, with the high value area mainly falling in the construction land, and the low value area located in the water bodies along the Yangtze River. (2) At the regional scale, the WY-CS interaction and WY-HQ interaction presented mainly trade-offs, while the HQ-CS interaction presented mainly synergistic, accounting for 11% of the total area. At the district scale, the ES tradeoff-synergy degree kept relatively high in the districts directly managed under Jiangbei New Area, while the ES tradeoff-synergy degree was relatively low in other districts. The change of construction land and water surface area has been identified as an important driving mechanism affecting the tradeoff or synergy of ESs.