Abstract: In the process of global biodiversity governance, the shared landscape between humans and wildlife has become a widespread reality. As wildlife populations gradually recover and human activities continue to expand, the spatial overlap between the two has been further intensified. With the establishment of the three major objectives of the Convention on Biological Diversity and the implementation of the "Kunming-Montreal Global Biodiversity Framework", global biodiversity governance has undergone a profound transformation from "fortress conservation" to "inclusive conservation", positioning human-wildlife coexistence as a central governance issue. Addressing the limitations of existing research that remains constrained to linear frameworks and lacks cross-case systematic comparison and integrative analytical frameworks, this paper employs systematic review and comparative analysis methods to examine the evolutionary patterns of biodiversity conservation and sustainable use pathways from a global governance perspective in the context of human-wildlife coexistence, and analyzes the triple blocking mechanisms of human-wildlife conflicts on sustainable use. Research findings indicate that the synergy between conservation and sustainable use does not rely on a single model but is achieved through the combination of four mechanisms: technological governance, market incentives, community empowerment, and cultural integration. The implementation effectiveness is significantly regulated by institutional conditions including governance structure, market development level, species characteristic and its cultural symbolism, and community organizational capacity. The core logic of coexistence governance has gradually evolved from "loss control" toward "benefit reconstruction and relationship reshaping". Building on this foundation, this paper constructs a three-dimensional analytical framework of "tools-conditions-effects" that systematically explains the mechanisms of action, applicable scenarios, and advantages and disadvantages of different pathways. The research results can provide theoretical support and policy implications for China's national park construction, improvement of community co-management mechanisms, realization of ecological product value, and biodiversity conservation and sustainable use, contributing to the governance goal of harmonious coexistence between humans and nature.