农药乙蒜素和细菌溴腈对蚕豆根尖细胞的致畸效应

Deforming Effect of Ethylicin and Bismerthiazol on Vicia faba Root Tip Cells

  • 摘要: 利用蚕豆根尖微核技术研究农药乙蒜素和细菌溴腈对蚕豆根尖细胞的遗传损伤,测定蚕豆根尖细胞的微核率、有丝分裂指数和染色体畸变率。结果表明:2种农药均能诱发较高频率的微核率、有丝分裂指数和染色体畸变率。在乙蒜素质量浓度为0.0320.160 g.L-1范围内,微核率随浓度升高而升高,呈一定的剂量-效应关系(r=0.881 9,P≤0.01);而在细菌溴腈质量浓度为0.6252.5 g.L-1范围内,微核率则呈先上升后下降的趋势。

     

    Abstract: Genotoxicity of ethylicin and bismerthiazol on Vicia faba root tip cells was studied.Micronucleus assay and chromosomal aberration assay were used to determine mitotic index,micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by different concentrations of ethylicin or bismerthiazol.Results show that both pesticides may induce high micronucleus frequency,mitotic index,and chromosomal aberration frequency.Micronucleus frequency increased with increasing of ethylicin concentration,and showed a linear dose-dependent manner in the treatment of ethylicin concentration ranging from 0.032 to 0.160 g·L-1.However,it increased first and then decreased with increasing bismerthiazol concentration in the treatment of its concentration ranging from 0.625 to 2.5 g·L-1.Different types of chromosomal aberration were induced by both ethylicin and bismerthiazol.

     

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