生态与农村环境学报 ›› 2015, Vol. 31 ›› Issue (5): 730-735.doi: 10.11934/j.issn.1673-4831.2015.05.018

• 污染控制与修复 • 上一篇    下一篇

促藻菌对3种微囊藻休眠体复苏的影响

邹万生,罗玉双,刘良国,王文彬,杨品红   

  1. 湖南文理学院生命科学学院
  • 收稿日期:2015-01-26 修回日期:2015-06-28 出版日期:2015-09-25 发布日期:2015-09-30
  • 通讯作者: 杨品红 E-mail:yph098@163.com
  • 作者简介:邹万生(1975-),男,湖南新化人,工程师,博士生,主要从事水环境生态与水污染控制技术方面的研究。E-mail:zwsksy@163.com
  • 基金资助:

    国家自然科学基金(31372519):湖南省科技厅项目(2012FJ3091);湖南省高校创新平台开放基金(12K121);动物学湖南省重点学科建设项目

Effects of two Algae-Promoting Bacteria on Anabiosis of Hypopuses of Three Kinds of Microcystis.

ZOU Wan-sheng,LUO Yu-shuang,LIU Liang-guo,WANG Wen-bin,YANG Pin-hong   

  1. Department of Life Science, Hunan University of Arts and Science
  • Received:2015-01-26 Revised:2015-06-28 Online:2015-09-25 Published:2015-09-30

摘要:

将促藻菌株Exiguobacterium sp.013(E.sp013)与 Bacillus sp.D06(B.spD06)复壮后和铜绿微囊藻、水华微囊藻和惠氏微囊藻的休眠体经底泥浸润包埋,进行底泥原位模拟试验,对比分析底泥表层2菌株对3种微藻休眠体复苏的影响。结果表明:在E.sp013作用下,铜绿微囊藻、水华微囊藻和惠氏微囊藻休眠体复苏的最低温度均降低1 ℃(对照组分别为11、12 和12 ℃),与对照组均存在显著性差异(独立样本T检验,P<0.05),而B.spD06并无此现象,但能提高3种微藻休眠体复苏率。同一条件下,E.sp013促3种微囊藻休眠体复苏强度强于B.spD06。E.sp013与B.spD06均能使微囊藻休眠体复苏时间提前,E.sp013和 B.spD06对铜绿微囊藻、水华微囊藻和惠氏微囊藻休眠体复苏时间均提前约1 d,2菌株在促3种微囊藻休眠体复苏时间的提前上并无显著性差异(Duncans multiple range test检验方法,P>0.05)。E.sp013与B.spD06通过自身对营养元素N的利用和去N能力降低了水体中的N/P比,这可能是促进3种微囊藻休眠体复苏的原因。

关键词: 促藻细菌, 微囊藻, 休眠体, 复苏

Abstract:

An in-situ simulation experiment was embedding rejuvenated algae-promoting strains of bacteria, Exiguobacterium sp.013 and Bacillus sp.D06, separately, with hypopuses of Microcystis aeruginosa, M.s flos-aqua and M. wesenbergii in wet sediment to compare effects of the formers on anabiosis of the latters Results show that as affected by E.sp013 the hypopuses of Microcystis aeruginosa, Microcystis flos-aqua and Microcystis wesenbergii started to resuscitate at a temperature 1℃ lower than that in their control groups, respectively. (11℃, 12℃ and 12℃, respectively), while B.spD06 did not have such an effect, but it did increase   resuscitation rates of the hypopuses. Under the same condition, E.sp013 was stronger than B.spD06 in effect of promoting resuscitation of the hypopuses. But both of them advanced resuscitation of the hypopuses in time by one day. No significant difference was found between the two strains of bacteria and between the three kinds of Microcystis in this effect (Duncans multiple range test, P> 0.05). The experiment also reveals that E.sp013 and B.spD06 bacteria reduced N\P ratio in the waterbody through their ability to assimilate N nutrient, thus removing N from the waterbody, which may be the reasons why the 2 strains of bacteria can promote resuscitation of the hypopuses of the 3 kinds of Microcystis.

Key words:  Algae-promoting bacteria, Microcystis, hypopus, resuscitation