基于psbA基因的噬藻体遗传多样性研究进展

doi:10.11934/j.issn.1673-4831.2016.03.013

生态与农村环境学报 ›› 2016, Vol. 32 ›› Issue (3): 417-423.doi: 10.11934/j.issn.1673-4831.2016.03.013

基于psbA基因的噬藻体遗传多样性研究进展

李樾, 刘婷婷, 刘丽, 夏雪山

昆明理工大学生命科学与技术学院, 云南昆明 650500

收稿日期:2015-08-31 出版日期:2016-05-25 发布日期:2016-05-27
通讯作者: 刘丽,E-mail:Liuli2272@163.com E-mail:Liuli2272@163.com
作者简介:李樾(1991-),女,黑龙江铁力人,硕士生,主要从事环境微生物学方面的研究。E-mail:184682604234@163.com
基金资助:
国家自然科学基金地区基金(30960005,31360065)

Progress of Research on Genetic Diversity of Cyanophage Based on psbA Gene

LI Yue, LIU Ting-ting, LIU Li, XIA Xue-shan

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China

Received:2015-08-31 Online:2016-05-25 Published:2016-05-27

摘要/Abstract

摘要：

噬藻体作为一类感染蓝藻的特异性病毒,广泛分布于不同水生态系统中。随着水体富营养化的加剧,蓝藻水华频繁爆发,具有潜在控藻能力的生物因子——噬藻体的研究备受关注。目前对于噬藻体的研究主要集中在其分离纯化及生物学特性等,因缺乏广泛通用的分子标记致使其遗传多样性研究受限。以编码光合作用反应中心D1蛋白的psbA基因为靶基因,综述了海洋、淡水环境中噬藻体psbA基因遗传多样性的最新研究进展,分析了噬藻体在不同自然环境中的分布及差异,同时也提出了噬藻体多样性研究中存在的一些问题。

关键词: 噬藻体, psbA基因, 遗传多样性

Abstract:

Cyanophages, as a kind of peculiar viruses that infect specifically cyanobacteria, exists extensively in various aquatic environments. In water bodies where water eutrophication is severe, cyanobacterial bloom outbreaks frequently. So scientists pay much attention to the study on cyanophages as a potential biological factor in controlling cyanobacteria. Currently studies on cyanophages are focused mainly on how to isolate and purify cyanophages and what its physiological and biochemical properties are. Little has been done on its genetic diversity because no universal genetic markers are available for the study. Using the photosynthesis psbA gene that encodes key photosystem II proteins (D1) of cyanophages as target, recent advances in researches on cyanophage genetic diversity in maritime and fresh water environments were reviewed and distribution and variation of cyanophages in various natural environments analyzed. Meanwhile, some tips for problems in and prospects of the researches in this field are also discussed.

Key words: cyanophage, psbA gene, genetic diversity

中图分类号:

李樾, 刘婷婷, 刘丽, 夏雪山. 基于psbA基因的噬藻体遗传多样性研究进展[J]. 生态与农村环境学报, 2016, 32(3): 417-423.

LI Yue, LIU Ting-ting, LIU Li, XIA Xue-shan. Progress of Research on Genetic Diversity of Cyanophage Based on psbA Gene[J]. Journal of Ecology and Rural Environment, 2016, 32(3): 417-423.

参考文献

[1] MATHIAS C B,KIRSCHNER A K T,VELIMIROV B.Seasonal Variations of Virus Abundance and Viral Control of the Bacterial Production in a Backwater System of the Danube River[J].Applied and Environmental Microbiology,1995,61(10):3734-3740.
[2] 向安,刘丽,魏大巧,等.噬藻体的分子生物学研究进展[J].生物技术通报,2009(5):31-34,38.
[3] 陈章然,郑伟,郑天凌.海洋藻类病毒多样性研究的现状与展望[J].地球科学前沿,2012,2:172-182.
[4] 沈浩,刘登义.遗传多样性的概述[J].生物学杂志,2001,18(3):5-8.
[5] 张奇亚.噬藻体生物多样性的研究动态[J].微生物学通报,2014,41(3):545-559.
[6] FULLER N G,WILSON W H,JOINT I R,et al.Occurrence of a Sequence in Marine Cyanophages Similar to That of T4 G20 and Its Application to PCR-Based Detection and Quantification Techniques[J].Applied and Environmental Microbiology,1998,64(6):2051-2060.
[7] ZHONG Y,CHEN F,WILHELM S W,et al.Phylogenetic Diversity of Marine Cyanophage Isolates and Natural Virus Communities as Revealed by Sequences of Viral Capsid Assembly Protein Gene G20[J].Applied and Environmental Microbiology,2002,68(4):1576-1584.
[8] MARSTON M F,SALLEE J L.Genetic Diversity and Temporal Variation in the Cyanophage Community Infecting Marine Synechococcus Species in Rhode Island's Coastal Waters[J].Applied and Environmental Microbiology,2003,69(8):4639-4647.
[9] JAMESON E,MANN N H,JOINT I,et al.The Diversity of Cyanomyovirus Populations along a North-South Atlantic Ocean Transect[J].The ISME Journal,2011,5(11):1713-1721.
[10] SHORT C M,SUTTLE C A.Nearly Identical Bacteriophage Structural Gene Sequences Are Widely Distributed in Both Marine and Freshwater Environments[J].Applied and Environmental Microbiology,2005,71(1):480-486.
[11] TAKASHIMA Y,YOSHIDA T,YOSHIDA M,et al.Development and Application of Quantitat Dievteection of Cyanophages Phylogenetically Related to Cyanophage Ma-LMM01 Infecting Microcystis aeruginosa in Fresh Water[J].Microbes and Environments,2007,22(3):207-213.
[12] YOSHIDA M,YOSHIDA T,KASHIMA A,et al.Ecological Dynamics of the Toxic Bloom-Forming Cyanobacterium Microcystis aeruginosa and Its Cyanophages in Freshwater[J].Applied and Environmental Microbiology,2008,74(10):3269-3273.
[13] CHEN F,WANG K,HUANG S,et al.Diverse and Dynamic Populations of Cyanobacterial Podoviruses in the Chesapeake Bay Unveiled Through DNA Polymerase Gene Sequences[J].Environmental Microbiology,2009,11(11):2884-2892.
[14] 高恶斌,宁德刚.噬藻体辅助代谢基因(AMGs)研究进展[J].微生物学通报,2014,41(8):1667-1674.
[15] SHARON I,BATTCHIKOVA N,ARO E M,et al.Comparative Metagenomics of Microbial Traits Within Oceanic Viral Communities[J].The ISME Journal,2011,5(7):1178-1190.
[16] SULLIVAN M B,LINDELL D,LEE J A,et al.Prevalence and Evolution of Core Photosystem Ⅱ Genes in Marine Cyanobacterial Viruses and Their Hosts[J].PLoS biology,2006,4(8):234.
[17] LINDELL D,JAFFE J D,JOHNSON Z I,et al.Photosynthesis Genes in Marine Viruses Yield Proteins During Host Infection[J].Nature,2005,438(7064):86-89.
[18] BAILEY S,CLOKIE M R,MILLARD A,et al.Cyanophage Infection and Photo-Inhibition in Marine Cyanobacteria[J].Research in Microbiology,2004,155(9):720-725.
[19] BRAGG J G,CHISHOLM S W.Modeling the Fitness Consequences of a Cyanophage Encoded Photosynthesis Gene[J].PLoS One,2008,3(10):3550.
[20] SHARON I,TZAHOR S,WILLIAMSON S,et al.Viral Photosynthetic Reaction Center Genes and Transcripts in the Marine Environment[J].The ISME Journal,2007,1(6):492-501.
[21] 王淼星,向安,魏大巧,等.噬藻体光合作用基因研究[J].生物技术通报,2010(1):14-18.
[22] ZEIDNER G,PRESTON C M,DELONG E F,et al.Molecular Diversity Among Marine Picophytoplankton as Revealed by psbA Analyses[J].Environmental Microbiology,2003,5(3):212-216.
[23] MILLARD A D,CLOKIE M R J,SHUB D A,et al.Genetic Organization of the psbAD Region in Phages Infecting Marine Synechococcus Strains[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(30):11007-11012.
[24] MILLARD A D,GIERGA G,CLOKIE M R J,et al.An Antisense RNA in a Lytic Cyanophage Links psbA to a Gene Encoding a Homing Endonuclease[J].The ISME Journal,2010,4(9):1121-1135.
[25] WANG K,CHEN F.Prevalence of Highly Host-Specific Cyanophages in the Estuarine Environment[J].Environmental Microbiology,2008,10(2):300-312.
[26] CHE'NARD C,SUTTLE C A.Phylogenetic Diversity of Sequences of Cyanophage Photosynthetic Gene psbA in Marine and Freshwaters[J].Applied and Environmental Microbiology,2008,74(17):5317-5324.
[27] SANDAA R A,CLOKIE M R J,MANN N H.Photosynthetic Genes in Viral Populations With a Large Genomic Size Range From Norwegian Coastal Waters[J].FEMS Microbiology Ecology,2008,63(1):2-11.
[28] BENCH S R,HANSON T E,WILLIANSON K E,et al.Metagenomic Characterization of Chesapeake Bay Virioplankton[J].Applied and Environmental Microbiology,2007,73(23):7629-7641.
[29] 卢龙飞.中国青岛近海、北黄海及渤海噬藻体遗传多样性研究[D].青岛:中国海洋大学,2013.
[30] ZHENG Q,JIAO N,ZHANG R,et al.The Evolutionary Divergence of psbA Gene in Synechococcus and Their Myoviruses in the East China Sea[J].PLoS ONE,2014,9(1):86644.
[31] SHORT C M,RUSANOVA O,Short S M.Quantification of Virus Genes Provides Evidence For Seed-Bank Populations of Phycodna Viruses in Lake Ontario,Canada[J].The ISME Journal,2011,5(5):810-821.
[32] ZHONG X,JACQUET S.Prevalence of Viral Photosynthetic and Capsid Protein Genes From Cyanophages in Two Large and Deep Perialpine Lakes[J].Applied and Environmental Microbiology,2013,79(23):7169-7178.
[33] WANG G,MURASE J,ASAKAWA S,et al.Novel Cyanophage Photosynthetic Gene psbA in the Floodwater of a Japanese Rice Field[J].FEMS Microbiology Ecology,2009,70(1):79-86.
[34] 荆瑞勇.东北稻田水体噬藻体基因多样性与蓝藻及其伴生菌的分离鉴定[D].长春:中国科学院东北地理与农业生态研究所,2014.

基于psbA基因的噬藻体遗传多样性研究进展

Progress of Research on Genetic Diversity of Cyanophage Based on psbA Gene

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价

[1]	李辛雷, 王洁, 殷恒福, 范正琪, 李纪元. 金花茶遗传多样性和遗传结构AFLP分析[J]. 生态与农村环境学报, 2019, 35(1): 63-68.
[2]	刘玮, 崔菁菁, 王翔宇, 李美真. 威海荣成鹿角菜野生群体遗传多样性及资源保护策略[J]. 生态与农村环境学报, 2012, 28(5): 500-504.
[3]	张中信, 张小平, 吴甘霖, 沈三保. 入侵植物加拿大一枝黄花和本地一枝黄花的遗传多样性比较[J]. 生态与农村环境学报, 2012, 28(2): 146-150.
[4]	杨永华, 钦佩, 胡永金. 分子遗传技术与生物遗传多样性研究[J]. 生态与农村环境学报, 1996, 12(4): 28-31,23.
[5]	金鉴明, 薛达元. 我国生物多样性及其保护战略[J]. 生态与农村环境学报, 1991, 7(2): 1-5.