表面活性剂强化油菜-微生物联合修复滴滴涕污染农田土壤研究

doi:10.11934/j.issn.1673-4831.2017.08.012

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (8): 755-761.doi: 10.11934/j.issn.1673-4831.2017.08.012

表面活性剂强化油菜-微生物联合修复滴滴涕污染农田土壤研究

吕良禾¹, 张鸿龄¹, 陈宗聪¹, 孙家君², 孙丽娜¹, 王晓旭¹

1. 沈阳大学污染环境的生态修复与资源化技术教育部重点实验室, 辽宁沈阳 110044;
2. 北京桑德环境工程有限公司, 北京 100000

收稿日期:2016-08-11 出版日期:2017-08-25 发布日期:2017-09-25
通讯作者: 张鸿龄,E-mail:zhl19792002@163.com;孙丽娜,E-mail:Sln629@163.com E-mail:zhl19792002@163.com;Sln629@163.com
作者简介:吕良禾(1991-),男,辽宁沈阳人,硕士生,主要从事土壤有机污染修复研究。E-mail:1435277042@qq.com
基金资助:
沈阳市科技计划（F14-133-9-00）；国家重点基础研究计划（2014CB441106）；辽宁省青年学者成长计划（LJQ2014134）

Surfactants Strengthen Cole-Microorganism for the Remediation of DDTs Contaminated Farmland Soils

LÜ Liang-he¹, ZHANG Hong-ling¹, CHEN Zong-cong¹, SUN Jia-jun², SUN Li-na¹, WANG Xiao-xu¹

1. Key Laboratory of Regional Environment and Eco-remediation(Shenyang University), Ministry of Education, Shenyang 110044, China;
2. Beijing Sander Environmental Engineering Co. Ltd., Beijing 100000, China

Received:2016-08-11 Online:2017-08-25 Published:2017-09-25

摘要/Abstract

摘要：

为了提高设施农业滴滴涕（DDTs）污染土壤的修复效果，通过田间实验研究不同浓度的混合化学表面活性剂（SDBS-TW80）和生物表面活性剂鼠李糖脂（RL）对油菜和甲基营养型芽孢杆菌（Bacillus methylotrophicus）联合去除设施农业土壤中DDTs的强化作用。结果表明，1个月后，单种油菜处理、接种降解菌和油菜-降解菌联合处理土壤中DDTs降解率分别为12.0%、38.2%和43.1%，显著高于对照处理。SDBS-TW80和RL均能不同程度地强化油菜-微生物对土壤中滴滴涕的去除效果。SDBS-TW80施加量为40 mg·kg^-1时设施农业土壤中滴滴涕降解率最高（56.5%），RL施加量为5 mg·kg^-1时降解率最高（65.7%），RL比SDBS-TW80更有利于提高DDTs污染土壤的生物修复效果。此外，当RL施加量为5 mg·kg^-1时对于毒性较强的p，p'-DDE也具有较好的降解效果，降解率高达69.5%。结果证实利用表面活性剂强化油菜联合甲基营养型芽孢杆菌现场修复DDTs污染土壤是可行的。考虑到修复效率和毒害作用，实际应用中应优先选用5 mg·kg^-1 RL组合。

关键词: 滴滴涕, 表面活性剂, 甲基营养型芽孢杆菌, 油菜, 污染农田土壤

Abstract:

In order to improve the remedial effects of DDTs contaminated soils in facility agriculture,a field experiment was conducted to enhance different concentrations of SDBS-TW80 and RL for bioremediation of DDTs contaminated soils by combining with cole and Bacillus methylotrophicus. The results show that the degradation rate of DDTs in soils by cole, degrading and cole-microorganism bacteria after one month were 12.0%, 38.2% and 43.1%, respectively, which was significantly higher than that in control treatment. SDBS-TW80 and RL could enhance the degradation rate of DDTs contaminated farmland soils by Bacillus methylotrophicus and cole. The highest degration rates of DDTs in farmland soils were 56.5% and 65.7%, respectively, when the concentration of SDBS-TW80 and RL was applied at 40 and 5 mg·kg^-1, respectively. RL showed higher degradation activity of DDTs than SDBS-TW80. Moreover, highest degradation rate of p,p'-DDE (the most toxic component) was about 69.5%, when the concentration of RL was applied at 5 mg·kg^-1. The results confirmed the workability of surfactants augmented remediation of DDT-contaminated soil by cole and Bacillus methylotrophicus. Considering the remediation efficiency and toxicity, RL was the best choice in practical application.

Key words: DDTs, surfactant, Bacillus methylotrophicus, cole, contaminated farmland soil

中图分类号:

吕良禾, 张鸿龄, 陈宗聪, 孙家君, 孙丽娜, 王晓旭. 表面活性剂强化油菜-微生物联合修复滴滴涕污染农田土壤研究[J]. 生态与农村环境学报, 2017, 33(8): 755-761.

LÜ Liang-he, ZHANG Hong-ling, CHEN Zong-cong, SUN Jia-jun, SUN Li-na, WANG Xiao-xu. Surfactants Strengthen Cole-Microorganism for the Remediation of DDTs Contaminated Farmland Soils[J]. Journal of Ecology and Rural Environment, 2017, 33(8): 755-761.

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表面活性剂强化油菜-微生物联合修复滴滴涕污染农田土壤研究

Surfactants Strengthen Cole-Microorganism for the Remediation of DDTs Contaminated Farmland Soils

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