功能植物内生细菌筛选及对多环芳烃降解效能研究

doi:10.19741/j.issn.1673-4831.2018.0112

生态与农村环境学报 ›› 2019, Vol. 35 ›› Issue (1): 83-90.doi: 10.19741/j.issn.1673-4831.2018.0112

功能植物内生细菌筛选及对多环芳烃降解效能研究

陶佳雨, 洪亚军, 陈雪梅, 刘文韬, 朱雪竹, 苗雅慧

南京农业大学资源与环境科学学院, 江苏南京 210095

收稿日期:2018-03-13 出版日期:2019-01-25 发布日期:2019-01-22
通讯作者: 朱雪竹 E-mail:zhuxuezhu@njau.edu.cn
作者简介:陶佳雨(1993-),女,江苏苏州人,硕士生,主要研究方向为环境污染控制及生物修复。E-mail:2015103042@njau.edu.cn
基金资助:
国家自然科学基金（31670514）；上海市"科技创新行动计划"长三角科技联合攻关领域项目（17295810601）

Isolation, Identification and PAH-Degrading Performance of an Endophytic Bacterium Enterobacter sp. PRd5

TAO Jia-yu, HONG Ya-jun, CHEN Xue-mei, LIU Wen-tao, ZHU Xue-zhu, MIAO Ya-hui

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China

Received:2018-03-13 Online:2019-01-25 Published:2019-01-22
Contact: 35 E-mail:zhuxuezhu@njau.edu.cn

摘要/Abstract

摘要：

功能植物内生细菌在防治土壤和植物多环芳烃（PAHs）污染方面具有潜力。从PAHs污染区采集了铁苋菜（Acalypha australis）、香附子（Cyperus rotundus）和麦冬（Ophiopogon japonicus）等健康植物样品，采用平板直接分离法获得8株能降解芘的植物内生细菌，主要为Enterobacter、Chitinophaga和Xanthomonas等菌属，其10 d对芘（50 mg·L^-1）的降解率为7.59%~45.50%。综合内生细菌在共代谢基质存在条件下的芘降解效能，选择从麦冬中分离出的Enterobacter sp.PRd5细菌为研究对象，探究其在各种环境条件下的芘降解性能。结果显示，Enterobacter sp.PRd5 10 d对芘（50 mg·L^-1）的降解率为41.37%~50.63%，7 d可降解95%以上的萘（500 mg·L^-1）、芴（100 mg·L^-1）和菲（50 mg·L^-1）等低分子量多环芳烃，10 d对荧蒽（50 mg·L^-1）和苯并[a]芘（10 mg·L^-1）等高分子量多环芳烃的降解率分别为35.89%和17.44%。降解芘的优化条件研究结果显示，Enterobacter sp.PRd5在pH值6.0~8.0、温度25~35℃、外加盐浓度0~10 g·L^-1、装液量10~30 mL·（100 mL）^-1、接种量3%~17%、芘初始质量浓度25~50 mg·L^-1和外加100 mg·L^-1葡萄糖条件下，可获得较高的芘降解效能。

关键词: 芘, 植物内生细菌, 分离筛选, 条件优化

Abstract:

Endophytic bacteria with PAH-degrading ability have shown great potential in reducing the PAH contamination in soils and plants. In this study, eight pyrene-degrading endophytic bacterial strains, belonging to Enterobacter sp., Chitinophaga sp., Xanthomonas sp. etc, were isolated from the inner plants growing in PAH-contaminated sites. The degradation rates of pyrene (50 mg·L^-1) by these strains ranged from 7.59% to 45.50% after 10-day cultivation under 30℃ and 150 r·min^-1. Having great potential in pyrene degradation, strain Enterobacter sp. PRd5 was selected as the representative for systematical investigation of its pyrene biodegradation ability under different culture conditions. The results show that 41.37%-50.63% of pyrene could be removed by Enterobacter sp. PRd5 from media after 10-day cultivation at 30℃ and 150 r·min^-1. More than 95% of naphthalene (500 mg·L^-1), fluorine (100 mg·L^-1), and phenanthrene (50 mg·L^-1), which are 2-3 ringed PAHs, were removed by Enterobacter sp. PRd5 after 7-day cultivation. Remarkably, 35.89% of fluoranthene (50 mg·L^-1) and 17.44% of benzo[a]pyrene (10 mg·L^-1) were removed by Enterobacter sp. PRd5 from the media after 10-day cultivation, suggesting that strain PRd5 has great capability of degrading 4-5 ringed PAHs. Furthermore, the study indicate that pH value of 6.0-8.0, temperature of 25-35℃, ρ(NaCl) ≤ 10 g·L^-1, inventory 10-30 mL·(100 mL)^-1 flask, 3%-17% inoculation quantity, initial pyrene concentration of 25-50 mg·L^-1, and 100 mg·L^-1 additional glucose are favorable for pyrene biodegradation by Enterobacter sp. PRd5.

Key words: pyrene, endophytic bacteria, isolation, PAH degradation

中图分类号:

X172
X53

陶佳雨, 洪亚军, 陈雪梅, 刘文韬, 朱雪竹, 苗雅慧. 功能植物内生细菌筛选及对多环芳烃降解效能研究[J]. 生态与农村环境学报, 2019, 35(1): 83-90.

TAO Jia-yu, HONG Ya-jun, CHEN Xue-mei, LIU Wen-tao, ZHU Xue-zhu, MIAO Ya-hui. Isolation, Identification and PAH-Degrading Performance of an Endophytic Bacterium Enterobacter sp. PRd5[J]. Journal of Ecology and Rural Environment, 2019, 35(1): 83-90.

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功能植物内生细菌筛选及对多环芳烃降解效能研究

Isolation, Identification and PAH-Degrading Performance of an Endophytic Bacterium Enterobacter sp. PRd5

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