十二烷基苯磺酸钠暴露对黄颡鱼(Pelteobagrus fulvidraco)胚胎发育毒性和仔鱼神经毒性的影响

doi:10.19741/j.issn.1673-4831.2023.0252

摘要/Abstract

摘要： 该研究旨在探明十二烷基苯磺酸钠(SDBS)对黄颡鱼(Pelteobagrus fulvidraco)胚胎发育毒性和仔鱼神经毒性的影响,科学评估水体中SDBS污染的环境生态风险。将受精后4 h的黄颡鱼胚胎暴露于质量浓度为0、0.2、0.4、0.6、0.8 mg·L^-1的SDBS中,观察胚胎孵化累计死亡率、畸形率;并通过检测5日龄仔鱼脑组织中乙酰胆碱酯酶活性及相关基因的转录水平、5-羟色胺及相关基因的转录水平,探讨其神经毒性的潜在机制。结果显示,与对照相比,暴露处理的黄颡鱼胚胎孵化畸形率提高4.5~14.5百分点(P＜0.05),死亡率提高7.8~23.4百分点(P＜0.05)。与对照相比,暴露处理正常仔鱼和畸形仔鱼的乙酰胆碱酯酶活性分别显著降低12.0%~50.5%和15.0%~53.9%(P＜0.05),暴露处理仔鱼乙酰胆碱酯酶基因mRNA转录水平下降;暴露处理正常仔鱼和畸形仔鱼5-羟色胺含量分别显著降低2.6%~9.9%和3.0%~11.1%(P＜0.05),暴露处理仔鱼5-羟色胺基因的转录水平降低。上述结果表明,十二烷基苯磺酸钠暴露显著提高了黄颡鱼胚胎孵化的死亡率和畸形率;SDBS通过抑制乙酰胆碱酯酶活性和5-羟色胺含量水平影响神经传导,抑制神经递质相关基因表达,从而对黄颡鱼产生胚胎发育毒性及神经毒性作用。

关键词: 十二烷基苯磺酸钠, 黄颡鱼, 神经毒性, 胚胎发育毒性, 仔鱼

Abstract: The aim of the paper is to understand the effects of sodium dodecyl benzene sulfonate (SDBS) on the embryo developmental toxicity and neurotoxicity of Pelteobagrus fulvidraco and to scientifically assess the environmental and ecological risks of SDBS contamination in water. The embryos of Pelteobagrus fulvidraco were exposed to SDBS at 0, 0.2, 0.4, 0.6, and 0.8 mg·L^-1 mass concentration for 4 h after fertilization to observe the cumulative mortality and malformation rate of embryos at hatching, and to explore the potential mechanism of neurotoxicity by detecting the transcript levels of acetylcholinesterase activity and related genes, 5-hydroxytryptamine levels and related genes in brain tissue of 5-day-old larvae. The results show that compared with the control group, the hatching malformation rate of exposed treated Pelteobagrus fulvidraco embryos increased by 4.5-14.5 percentages (P＜0.05) and the mortality rate increased by 7.8-23.4 percentages (P＜0.05). The acetylcholinesterase activity in exposed normal and deformed littermates was significantly reduced by 12.0%-50.5% (P＜0.05) and 15.0%-53.9% (P＜0.05), respectively. And the mRNA expression level of acetylcholinesterase gene decreased in exposed littermates. The 5-hydroxytryptamine content in exposed normal and deformed littermates was significantly reduced by 2.6%-9.9% and 3.0%-11.1% (P＜0.05), respectively. The transcript levels of 5-hydroxytryptamine gene were significantly reduced in normal and deformed fish. The above results indicate that SDBS exposure significantly increased the mortality and malformation rate of Pelteobagrus fulvidraco embryos at hatching. SDBS affected neurotransmission and inhibited neurotransmitter-related gene expression by inhibiting acetylcholinesterase activity and 5-hydroxytryptamine level, thus producing embryo developmental toxic and neurotoxic effects on Pelteobagrus fulvidraco.

Key words: sodium dodecyl benzene sulfonate, Pelteobagrus fulvidraco, neurotoxicity, embryo developmental toxicity, larvae

中图分类号:

X171.5

田海军, 姜汉军, 任胜杰, 杨治国. 十二烷基苯磺酸钠暴露对黄颡鱼(Pelteobagrus fulvidraco)胚胎发育毒性和仔鱼神经毒性的影响[J]. 生态与农村环境学报, 2024, 40(4): 541-547.

TIAN hai-jun, JIANG Han-jun, REN sheng-jie, YANG zhi-guo. Effect of Sodium Dodecyl Benzene Sulfonate Exposure on Embryo Developmental Toxicity and Neurotoxicity of Larvae Pelteobagrus fulvidraco[J]. Journal of Ecology and Rural Environment, 2024, 40(4): 541-547.

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