四溴双酚A和2,4,6-三溴苯酚对黄颡鱼的内分泌干扰毒性效应

doi:10.11934/j.issn.1673-4831.2016.06.023

生态与农村环境学报 ›› 2016, Vol. 32 ›› Issue (6): 1012-1017.doi: 10.11934/j.issn.1673-4831.2016.06.023

四溴双酚A和2,4,6-三溴苯酚对黄颡鱼的内分泌干扰毒性效应

张圣新^1,2, 刘济宁², 王蕾², 杨先海², 石利利^1,2, 刘丹²

1. 南京信息工程大学环境科学与工程学院, 江苏南京 210044;
2. 环境保护部南京环境科学研究所, 江苏南京 210042

出版日期:2016-11-25 发布日期:2016-11-30
通讯作者: 王蕾,E-mail:wanglei@nies.org E-mail:wanglei@nies.org
作者简介:张圣新(1992-),女,江苏镇江人,硕士生,主要从事化学品生态毒理学研究。E-mail:15195811701@163.com
基金资助:
江苏省自然科学基金（BK20151100）；普通高校研究生科研创新计划（KYLX15_0798）

Endocrine Disrupting Effects of TBBPA and TBP on Pelteobagrus fulvidraco

ZHANG Sheng-xin^1,2, LIU Ji-ning², WANG Lei², YANG Xian-hai², SHI Li-li^1,2, LIU Dan²

1. School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China;
2. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China

Online:2016-11-25 Published:2016-11-30

摘要/Abstract

摘要：

为研究四溴双酚A（TBBPA）及其降解产物2，4，6-三溴苯酚（TBP）对黄颡鱼（Pelteobagrus fulvidraco）的内分泌干扰毒性效应，在急性毒性试验的基础上开展黄颡鱼幼体生长毒性试验，分析黄颡鱼的生长率并推导出TBBPA和TBP对黄颡鱼28 d最低可观察效应浓度（MORC）和无可观察效应浓度（URC）；利用酶联免疫吸附（ELISA）试剂盒分析测定黄颡鱼内脏促肾上腺皮质激素（ACTH）和卵黄蛋白原（VTG）含量，并进行差异显著性分析。结果表明，受试物的浓度和黄颡鱼体重增长速率呈明显的剂量-效应关系，TBBPA对黄颡鱼体重增长率的MORC和URC分别为0.32和0.16 mg·L^-1，TBP对黄颡鱼体重增长率的MORC和URC分别为0.64和0.32 mg·L^-1，TBBPA较TBP具有更高的毒性。随着TBBPA浓度的升高，黄颡鱼内脏中ACTH和VTG含量与对照相比总体呈上升趋势。TBP对黄颡鱼内脏中VTG无显著影响，但在其质量浓度为0.64 mg·L^-1时可显著降低ACTH含量。推测TBBPA和TBP是潜在的环境内分泌干扰物。

关键词: 四溴双酚A, 2,4,6-三溴苯酚, 促肾上腺皮质激素, 卵黄蛋白原, 黄颡鱼, 内分泌干扰

Abstract:

Based on an acute toxicity test on fish, an experiment was conducted on toxicity of tetrabromobisphenol A (TBBPA) and its degradation product of 2,4,6-tribromophenol (TBP) to growing young Pelteobagrus fulvidraco, especially their endocrine-disrupting effects. During the experiment, growth rate of the juveniles was measured and the minimum observable response concentration (MORC) and unobservable response concentration (URC) of TBBPA and TBP in the juveniles exposed to the chemicals for 28 days. Contents of vitellogenin (VTG) and adrenocorticotropic hormone (ACTH) content in viscera of the fish were also measured using the enzyme-linked immunosorbent assay (ELISA) kits for significant difference analysis. Results show that concentrations of TBBPA and TBP and growth rate of the fish in weight displayed an obvious does-effect relationship with MORC and URC of TBBPA being 0.32 and 0.16 mg·L^-1 and MORC and URC of TBP being 0.64 and 0.32 mg·L^-1, respectively, which indicates that TBBPA was more toxic than TBP. With rising TBBPA concentration, VTG and ACTH in the fish significantly increased in content, but VTG did not show any significant response to change in concentration of TBP and ACTH declined by a large margin in content when TBP reached 0.64 mg·L^-1 in concentration. It is, therefore, presumed that TBBPA and TBP are potential environmental endocrine disruptors.

Key words: tetrabromobisphenol A (TBBPA), 2,4,6-tribromophenol (TBP), adrenocorticotropic hormone (ACTH), vitellogenin (VTG), Pelteobagrus fulvidraco, endocrine disrupting

中图分类号:

X592

张圣新, 刘济宁, 王蕾, 杨先海, 石利利, 刘丹. 四溴双酚A和2,4,6-三溴苯酚对黄颡鱼的内分泌干扰毒性效应[J]. 生态与农村环境学报, 2016, 32(6): 1012-1017.

ZHANG Sheng-xin, LIU Ji-ning, WANG Lei, YANG Xian-hai, SHI Li-li, LIU Dan. Endocrine Disrupting Effects of TBBPA and TBP on Pelteobagrus fulvidraco[J]. Journal of Ecology and Rural Environment, 2016, 32(6): 1012-1017.

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四溴双酚A和2,4,6-三溴苯酚对黄颡鱼的内分泌干扰毒性效应

Endocrine Disrupting Effects of TBBPA and TBP on Pelteobagrus fulvidraco

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