生态与农村环境学报 ›› 2004, Vol. 20 ›› Issue (4): 48-50.doi:

• 论文 • 上一篇    下一篇

甲基对硫磷长期污染对土壤微生物的生态效应

张瑞福, 崔中利, 何健, 黄婷婷, 李顺鹏   

  1. 南京农业大学生命科学学院微生物学系
  • 收稿日期:2004-02-11 出版日期:2004-10-25 发布日期:2011-10-26
  • 通讯作者: 李顺鹏 南京农业大学生命科学学院微生物学系
  • 作者简介:张瑞福(1974-),男,山东泰安人,博士,主要从事土壤和环境微生物研究。
  • 基金资助:

    "十五"国家科技攻关计划项目(2002BA516A01)

Ecological effects of long-term methylparathion contamination on soil microflora

ZHANG  Rui-Fu, CUI  Zhong-Li, HE  Jian, HUANG  Ting-Ting, LI  Shun-Peng   

  1. Department of Microbiology,College of Life Science,Nanjing Agricultvironment,Ministry of Agriculture
  • Received:2004-02-11 Online:2004-10-25 Published:2011-10-26
  • Contact: LI Shun-Peng Department of Microbiology,College of Life Science,Nanjing Agricultvironment,Ministry of Agriculture

摘要: 研究了有机磷农药甲基对硫磷长期污染对土壤微生物的影响,结果表明:污染土壤中细菌、放线菌、真菌数量变化不大,自生固氮菌和反硝化细菌数量减少,氨化细菌、亚硝化细菌、硝化细菌数量有所增加;与对照土壤相比,污染土壤呼吸作用下降29.93%,而氨化作用和硝化作用则增强。1g对照土壤(以干土计)中微生物总DNA提取量为(13.01±1.91)μg,污染土壤中则为(12.23±1.36)μg。

关键词: 甲基对硫磷, 长期污染, 土壤微生物, 生态效应

Abstract: Long-term effects of methylparathion contamination on soil microflora were investigated.Little difference in total counts of bacteria,actinomycetes and fungi was observed between the contaminated and the non-contaminated soil.Compared with the control,the contaminated soil was slightly lower in total count of azotobacters and denitrifying bacteria and significantly higher in those of ammonifying and ammonia-oxidizing and nitrifying bacteria.The respiratory intensity of the contaminated soil decreased by 29.93% while ammonification and nitrification increased significantly over that of control soil.The total DNA extracted from the contaminated soil and the control was (12.23±1.36)μg·g-1 and (13.01±(1.91))μg·g-1 dry soil,respectively.

Key words: methylparathion, long-term contamination, soil microorganism, ecological effect

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