有机肥与化肥配施对黄瓜产量及土壤微生物多样性的影响

doi:10.11934/j.issn.1673-4831.2016.05.013

Abstract

Abstract:

Combined application of chemical fertilizer with organic manure has obvious advantages over application of chemical fertilizer or organic manure alone in terms of crop yield, but little has been reported about effect of the combination on soil microbial diversity. A greenhouse experiment was conducted to investigate effects of the combined application of chemical fertilizer with organic manure on cucumber yield and soil microbial diversity. The experiment was designed to have 5 treatments, that is, Treatment CK (no fertilization), Treatment CF[Chemical fertilizer w(N):w(P₂O₅):w(K₂O)=15:15:15], Treatment WOF (NPK+water hyacinth), Treatment POF (NPK+pig manure) and Treatment FOF (NPK+fermented bedding). All the treatments, except for Treatment CK, were the same in N input and the combination followed 1:1 ratio. Results show that the treatments of combined application were higher than or equal to Treatment CF in cucumber yield. Treatment FOF in particular was 26.77% higher, and followed by Treatment WOF. The combined fertilization was found to be able to maintain or raise cucumber yield and improve quality of the fruit as well. The cucumbers from the treatments of combined fertilization were about 30% higher than those from Treatment CF in Vitamin C content. The combined fertilization also improved nitrogen use efficiency, but the effect varied sharply from treatment to treatment. PCR-DGGE shows that soil bacteria community structure in the five treatments could be classified into three groups. Treatments CK and CF were quite similar in soil bacterial community structure and Treatments WOF and POF were similar too, while Treatment FOF was unique. The treatments of combined fertilization, regardless of source of organic manure, all increased the Shannon-Wiener index and abundance of soil bacteria by as far as over 5% as compared with Treatment CF, which indicates that application of extraneous organic matter could alter structure of the soil microbial community and improve soil microbial diversity, but the application of chemical fertilizer (CF) has little effect on the soil microbial community structure.

Key words: organic manure, chemical fertilizer, combined fertilization, cucumber, soil microbial diversity

CLC Number:

X53
S141.2

LUO Jia, LIU Li-zhu, WANG Tong, YAN Shao-hua, LU Xin, FAN Ru-qin, ZHANG Zhen-hua. Effect of Combined Application of Chemical Fertilizer With Organic Manure on Cucumber Yield and Soil Microbial Diversity[J]. Journal of Ecology and Rural Environment, 2016, 32(5): 774-779.

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Effect of Combined Application of Chemical Fertilizer With Organic Manure on Cucumber Yield and Soil Microbial Diversity

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