表面活性剂强化油菜-微生物联合修复滴滴涕污染农田土壤研究

doi:10.11934/j.issn.1673-4831.2017.08.012

Abstract

Abstract:

In order to improve the remedial effects of DDTs contaminated soils in facility agriculture,a field experiment was conducted to enhance different concentrations of SDBS-TW80 and RL for bioremediation of DDTs contaminated soils by combining with cole and Bacillus methylotrophicus. The results show that the degradation rate of DDTs in soils by cole, degrading and cole-microorganism bacteria after one month were 12.0%, 38.2% and 43.1%, respectively, which was significantly higher than that in control treatment. SDBS-TW80 and RL could enhance the degradation rate of DDTs contaminated farmland soils by Bacillus methylotrophicus and cole. The highest degration rates of DDTs in farmland soils were 56.5% and 65.7%, respectively, when the concentration of SDBS-TW80 and RL was applied at 40 and 5 mg·kg^-1, respectively. RL showed higher degradation activity of DDTs than SDBS-TW80. Moreover, highest degradation rate of p,p'-DDE (the most toxic component) was about 69.5%, when the concentration of RL was applied at 5 mg·kg^-1. The results confirmed the workability of surfactants augmented remediation of DDT-contaminated soil by cole and Bacillus methylotrophicus. Considering the remediation efficiency and toxicity, RL was the best choice in practical application.

Key words: DDTs, surfactant, Bacillus methylotrophicus, cole, contaminated farmland soil

CLC Number:

LÜ Liang-he, ZHANG Hong-ling, CHEN Zong-cong, SUN Jia-jun, SUN Li-na, WANG Xiao-xu. Surfactants Strengthen Cole-Microorganism for the Remediation of DDTs Contaminated Farmland Soils[J]. Journal of Ecology and Rural Environment, 2017, 33(8): 755-761.

References

[1] 吴鹏.机械化学法处理高浓度滴滴涕污染土壤的初步研究[D].天津:天津大学,2014.[WU Peng.Preliminary Study on Mechanochemical Treatment of Soil Heavily Contaminated With DDT[D].Tianjin:Tianjin University,2014.]
[2] HU W Y,LU Y L,WANG T Y,et al.Spatial Variability and Temporal Trends of HCH and DDT in Soils Around Beijing Guanting Reservoir,China[J].Environmental Geochemistry and Health,2010,32(5):441-449.
[3] SUDHARSHAN S,NAIDU R,MALLAVARAPU M,et al.DDT Remediation in Contaminated Soils:A Review of Recent Studies[J].Biodegradation,2012,23(6):851-863.
[4] 陈丽蓉,严志辉,陈小罗,等.农药DDT在土壤中迁移转化研究[J].科技创新导报,2011,32:123,125.[CHEN Li-rong,YAN Zhi-hui,CHEN Xiao-luo,et al.Pesticide DDT Migration Transformation in the Soil[J].Science and Technology Innovation Herald,2011,32:123,125.]
[5] LIMA T M S,PROCÓPIO L C,BRANDÄO F D,et al.Biodegradability of Bacterial Surfactants[J].Biodegradation,2011,22(3):585-592.
[6] 刘文斌.DDTs好氧降解菌株的筛选及其降解特性研究[D].大连:大连理工大学,2009.[LIU Wen-bin.Isolation,Identification and Characterization of a DDTs-Degrading Bacterium[D].Dalian:Dalian University of Technology,2009.]
[7] GURSKA J,WANG W X,KAREN E G,et al.Three Year Field Test of a Plant Growth Promoting Rhizobacteria Enhanced Phytoremediation System at a Land Farm for Treatment of Hydrocarbon Waste[J].Environmental Science & Technology,2009,43(12):4472-4479.
[8] 陈伟伟.表面活性剂对污染场地土壤中DDTs的增溶洗脱作用研究[D].南京:南京农业大学,2009.[CHEN Wei-wei.Solubilization and Washing of DDTs From Contaminated Site Soil With Surfactant[D].Nanjing:Nanjing Agricultural University,2009.]
[9] 李登宇.表面活性剂强化微生物修复DDT污染土壤研究[D].沈阳:沈阳大学,2016.[LI Deng-yu.Study on Enhancing Microbe Remediation of DDT Contaminated Soil by Surfactants[D].Shengyang:Shenyang University,2016.]
[10] 肖鹏飞,秦必达,李玉文.非离子表面活性剂强化白腐菌及其粗酶液对有机氯农药的降解[J].应用与环境生物学报,2015,21(1):22-28.[XIAO Peng-fei,XIN Bi-da,LI Yu-wen.Effects of Nonionic Surfactants on Degradation of Organochlorine Pesticides by White Rot Fungus and Its Enzyme[J].Chinese Journal of Applied Environmental Biology,2015,21(1):22-28.]
[11] FANG H,DONG B,YAN H,et al.Characterization of a Bacterial Strain Capable of Degrading DDT Congeners and Its Use in Bioremediation of Contaminated Soil[J].Journal of Hazardous Materials,2010,184(1/2/3):281-289.
[12] 张岩,张景发,陈艳梅,等.加速溶剂萃取-气相色谱法测定土壤中有机氯农药和多氯联苯[J].岩矿测试,2010,29(5):491-496.[ZHANG Yan,ZHANG Jing-fa,CHEN Yan-mei,et al.Determination of Organochlorine Pesticides and Polychlorinated Biphenyls in Soils by Gas Chromatography With Accelerated Solvent Extraction[J].Rock and Mineral Analysis,2010,29(5):491-496.]
[13] GB/T 14550-2003,土壤中六六六和滴滴涕测定的气相色谱法[S].[GB/T 14550-2003,Method of Gas Chromatographic for Determination of BHC and DDT in Soil[S].]
[14] 安凤春,莫汉宏,郑明辉,等.DDT及其主要降解产物污染土壤的植物修复[J].环境化学,2003,22(1):19-25.[AN Feng-chun,MO Han-hong,ZHENG Ming-hui,et al.Phytoremediation of DDT and Its Main Degradation Product-Contaminated Soil Using Grass[J].Environmental Chemistry,2003,22(1):19-25.]
[15] 朱白澍,梁涛.我国设施农业土壤障碍现状[J].磷肥与复合肥,2010,25(3):73-75.[ZHU Bai-shu,LIANG Tao.Status of Soil Obstacle of Facility Agriculture in China[J].Phosphate & Compound Fertilizer,2010,25(3):73-75.]
[16] GLICK B,WHATLEY S.The Effect of o,p-DDD in the Chicken[J].Experientia,1966,22(3):179-180.
[17] 肖鹏飞,李玉文,KONDO R.Tween 60和SDS强化白腐真菌修复DDT污染土壤[J].中国环境科学,2015,35(12):3737-3743.[XIAO Peng-fei,LI Yu-wen,KONDO R.Enhancement of Tween 60 and SDS for Bioremediation of DDT-Contaminated Soil by White Rot Fungus[J].China Environmental Science,2015,35(12):3737-3743.]
[18] LIANG Qi,LEI Mei,CHEN Tong-bin,et al.Application of Sewage Sludge and Intermittent Aeration Strategy to the Bioremediation of DDT-and HCH-Contaminated Soil[J].Journal of Environmental Sciences,2014,26(8):1673-1680.
[19] 赵炳梓,张佳宝,周凌云,等.黄淮海地区典型农业土壤中六六六(HCH)和滴滴涕(DDT)的残留量研究Ⅰ:表层残留量及其异构体组成[J].土壤学报,2005,42(5):59-66.[ZHAO Bing-zi,ZHANG Jia-bao,ZHOU Ling-yun,et al.Residues of HCH and DDT in Typical Agricultural Soils of Huang-Huai-Hai Plain,CHINAⅠ:Residues in Surface Soils and Thier Isomeric Composition[J].Acta Pedologica Sinica,2005,42(5):59-66.]
[20] RūTA I G,JUOZAS K.Effects of Rhamnolipid Biosurfactant JBR425 and Synthetic Surfactant Surfynol465 on the Peroxidase-Catalyzed Oxidation of 2-Naphthol[J].Journal of Environmental Sciences,2013,25(7):1431-1440.
[21] 沈薇.生物修复环境污染的微生物筛选及其性能研究[D].南京:南京理工大学,2006.[SHEN Wei.Bioremediation of Microbial Screening of Environmental Pollution and Its Performance Study[D].Nanjing:Nanjing University of Science and Technology,2006.]
[22] 李果,毛华军,巩宗强,等.几种表面活性剂对柴油及多环芳烃的增溶作用[J].环境科学研究,2011,24(7):775-780.[LI Guo,MAO Hua-jun,GONG Zong-qiang,et al.Solubilization of Diesel and Polycyclic Aromatic Hydrocarbons by Certain Kinds of Surfactants[J].Research of Environmental Sciences,2011,24(7):775-780.]
[23] 龚丽芬,蔡建秀,谢晓兰.Y-TiO₂光催化降解高效氟氯氰菊酯和p,p'-DDE[J].宁德师范学院学报(自然科学版),2013,25(4):353-355.[GONG Li-fen,CAI Jian-xiu,XIE Xiao-lan.Photocatalytic Degradation of the Cyfluthrin and p,p'-DDE by Y-TiO₂[J].Journal of Ningde Normal University (Natural Science),2013,25(4):353-355.]

Surfactants Strengthen Cole-Microorganism for the Remediation of DDTs Contaminated Farmland Soils

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