长期不同施肥模式对农田黑土微生物群落构建的影响

doi:10.19741/j.issn.1673-4831.2020.0807

Abstract

Abstract: Intensive agricultural management is influential to soil microbiome and its functions. Based on a 36-year fertilization experiment at the Ministry of Agriculture Key Field Observation Station of Harbin Black Soil Ecological Environment, effects of four fertilization managements (CK:no fertilizer; NPK:mineral fertilizer; OM:organic fertilizer; MNPK:mineral NPK plus organic fertilizer) on soil fertility and microbial respiration were quantitively studied, and microbial community assembly mechanisms were analyzed using the high-throughput sequencing and community assembly models. Results show that long-term fertilization improved soil fertility, such as soil organic carbon, total nitrogen, available phosphorus and microbial activity. Soil microbial respiration was increased in the order of CK-1), soil microbial respiration in MNPK (calculated in carbon, 228.7 μg·g^-1) was increased by 22.4%. Linear discriminant analysis effect size (LEfSe) indicates that NPK and MNPK treatments had similar keystones including Dyella marensis, Bacillus megaterium and Herbaspirillum sp., while OM was predominated by Bacillus acidiceler and Bacillus megaterium. As compared with CK, long-term fertilization stimulated the growth of ammonia oxidizing bacteria (AOB) with the Nitrosospira-like AOB increased by 3.61 times in NPK treatment. The dominant phylotypes of ammonia oxidizing archaea (AOA) and nitrite oxidizing bacteria (NOB) were Nitrososphaera and Nitrospira, respectively. The co-occurrence network analysis revealed that soil microbial community in MNPK had the most closely links, with the proportion of positive links reached 99.6% versus 78.4% in NPK and 97.4% in OM. The analysis of β NTI indicates that long-term fertilization strengthened the deterministic processes of microbial community assembly, with the strongest determinism occurred in MNPK. Structural equation models (SEMs) further suggest strongly positive correlations between soil fertility improvement and microbial respiration (explaining > 90% of variation) among fertilization managements. This study demonstrates that long-term fertilization significantly stimulated the growth of functional microbiomes, altered community structure and improved soil fertility. Compared with NPK and OM, MNPK treatment was more efficient in enhancing soil fertility and stability of microbial community. As a whole, these results provide scientific basis for optimizing the efficient fertilization managements in black soils.

Key words: black soil, long-term fertilization, high-throughput sequencing, community assembly, keystone phylotypes

CLC Number:

S154

GAO Wei, WANG Lian-feng, JIA Zhong-jun. Changes in Community Assembly of Microbiomes in Black Soil under Distinct Scenarios of Long-term Field Fertilization[J]. Journal of Ecology and Rural Environment, 2021, 37(11): 1437-1448.

References

[1] 汪景宽, 李双异, 张旭东, 等.20年来东北典型黑土地区土壤肥力质量变化[J]. 中国生态农业学报, 2007, 15(1):19-24.[WANG Jing-kuan, LI Shuang-yi, ZHANG Xu-dong, et al. Spatial and Temporal Variability of Soil Quality in Typical Black Soil Area in Northeast China in 20 Years[J]. Chinese Journal of Eco-agriculture, 2007, 15(1):19-24.]
[2] OU Y, ROUSSEAU A N, WANG L X, et al. Spatio-temporal Patterns of Soil Organic Carbon and pH in Relation to Environmental Factors:A Case Study of the Black Soil Region of Northeastern China[J]. Agriculture, Ecosystems & Environment, 2017, 245:22-31.
[3] 韩晓增, 邹文秀.我国东北黑土地保护与肥力提升的成效与建议[J]. 中国科学院院刊, 2018, 33(2):206-212.[HAN Xiaozeng, ZOU Wen-xiu. Effects and Suggestions of Black Soil Protection and Soil Fertility Increase in Northeast China[J]. Bulletin of Chinese Academy of Sciences, 2018, 33(2):206-212.]
[4] BAI Z G, CASPARI T, GONZALEZ M R, et al.Effects of Agricultural Management Practices on Soil Quality:A Review of Long-term Experiments for Europe and China[J]. Agriculture, Ecosystems & Environment, 2018, 265:1-7.
[5] MÄDER P, FLIESSBACH A, DUBOIS D, et al. Soil Fertility and Biodiversity in Organic Farming[J]. Science, 2002, 296(5573):1694-1697.
[6] LUO G W, LI L, FRIMAN V P, et al. Organic Amendments Increase Crop Yields by Improving Microbe-mediated Soil Functioning of Agroecosystems:A Meta-analysis[J]. Soil Biology and Biochemistry, 2018, 124:105-115.
[7] BANDYOPADHYAY K K, MISRA A K, GHOSH P K, et al.Effect of Integrated Use of Farmyard Manure and Chemical Fertilizers on Soil Physical Properties and Productivity of Soybean[J]. Soil and Tillage Research, 2010, 110(1):115-125.
[8] HERMANS S M, BUCKLEY H L, CASE B S, et al.Using Soil Bacterial Communities to Predict Physico-chemical Variables and Soil Quality[J]. Microbiome, 2020, 8(1):79.
[9] HUBBELL S P. TreeDispersion, Abundance, and Diversity in a Tropical Dry Forest[J]. Science, 1979, 203(4387):1299-1309.
[10] DINI-ANDREOTE F, STEGEN J C, VAN ELSAS J D, et al.Disentangling Mechanisms that Mediate the Balance between Stochastic and Deterministic Processes in Microbial Succession[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(11):E1326-E1332.
[11] VELLEND M.Conceptual Synthesis in Community Ecology[J]. The Quarterly Review of Biology, 2010, 85(2):183-206.
[12] CHASE J M, MYERS J A.Disentangling the Importance of Ecological Niches from Stochastic Processes across Scales[J]. Philosophical Transactions of the Royal Society of London Series B:Biological Sciences, 2011, 366(1576):2351-2363.
[13] STEGEN J C, LIN X, FREDRICKSON J K, et al. Estimating and Mapping Ecological Processes Influencing Microbial Community Assembly[J]. Frontiers in Microbiology, 2015, 6:370.
[14] SHI Y, LI Y, XIANG X, et al. Spatial Scale Affects the Relative Role of Stochasticity versus Determinism in Soil Bacterial Communities in Wheat Fields across the North China Plain[J]. Microbiome, 2018, 6(1):27.
[15] 鲍士旦.土壤农化分析[M]. 2版.北京:中国农业出版社, 2010:25-114.[BAO Shi-dan.Soil and Agricultural Chemistry Analysis[M]. 2nd ed.Beijing:Chinese Agriculture Press, 2010:25-114.]
[16] GAO W, XU J S, ZHAO J, et al.Prokaryotic Community Assembly after 40 Years of Soda Solonetz Restoration by Natural Grassland and Reclaimed Farmland[J]. European Journal of Soil Biology, 2020, 100:103213.
[17] STUBNER S. Enumeration of 16S rDNA of Desulfotomaculum Lineage 1 in Rice Field Soil by Real-time PCR with SybrGreenTM Detection[J]. Journal of Microbiological Methods, 2002, 50(2):155-164.
[18] POLY F, MONROZIER L J, BALLY R.Improvement in the RFLPprocedure for Studying the Diversity of nifH Genes in Communities of Nitrogen Fixers in Soil[J]. Research in Microbiology, 2001, 152(1):95-103.
[19] MASUDA T, INOMURA K, TAKAHATA N, et al. Heterogeneous Nitrogen Fixation Rates Confer Energetic Advantage and Expanded Ecological Niche of Unicellular Diazotroph Populations[J]. Communications Biology, 2020, 3:172.
[20] ZHOU J, GUAN D W, ZHOU B K, et al.Influence of 34-years of Fertilization on Bacterial Communities in an Intensively Cultivated Black Soil in Northeast China[J]. Soil Biology and Biochemistry, 2015, 90:42-51.
[21] SAINI V K, BHANDARI S C, TARAFDAR J C. Comparison of Crop Yield, Soil Microbial C, N and P, N-fixation, Nodulation and Mycorrhizal Infection in Inoculated and Non-inoculated Sorghum and Chickpea Crops[J]. Field Crops Research, 2004, 89(1):39-47.
[22] WEMHEUER F, KAISER K, KARLOVSKY P, et al.Bacterial Endophyte Communities of Three Agricultural Important Grass Species Differ in Their Response towards Management Regimes[J]. Scientific Reports, 2017, 7:40914.
[23] FIERER N, BRADFORD M A, JACKSON R B. Toward an Ecological Classification of Soil Bacteria[J]. Ecology, 2007, 88(6):1354-1364.
[24] JIA Z J, CONRAD R.Bacteria Rather than Archaea Dominate Microbial Ammonia Oxidation in an Agricultural Soil[J]. Environmental Microbiology, 2009, 11(7):1658-1671.
[25] NICOL G W, LEININGER S, SCHLEPER C, et al.The Influence of Soil pH on the Diversity, Abundance and Transcriptional Activity of Ammonia Oxidizing Archaea and Bacteria[J]. Environmental Microbiology, 2008, 10(11):2966-2978.
[26] SUN D L, JIANG X, WU Q L, et al.Intragenomic Heterogeneity of 16S rRNA Genes Causes Overestimation of Prokaryotic Diversity[J]. Applied and Environmental Microbiology, 2013, 79(19):5962-5969.
[27] FENG Y Z, CHEN R R, STEGEN J C, et al.Two Key Features Influencing Community Assembly Processes at Regional Scale:Initial State and Degree of Change in Environmental Conditions[J]. Molecular Ecology, 2018, 27(24):5238-5251.
[28] LING N, ZHU C, XUE C, et al.Insight into How Organic Amendments Can Shape the Soil Microbiome in Long-term Field Experiments as Revealed by Network Analysis[J]. Soil Biology and Biochemistry, 2016, 99:137-149.

Changes in Community Assembly of Microbiomes in Black Soil under Distinct Scenarios of Long-term Field Fertilization

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FENG Lin, LEI Guo-ping. The Influence of Rural Settlements on Soil Erosion in the Typical Black Soil Region of Northeast China [J]. Journal of Ecology and Rural Environment, 2021, 37(8): 1011-1021.
[2]	LI Xue, LIU Shu-feng, YAN Yu-meng, ZHANG Run-jie, WANG Tian-jie, FU Bao-rong. Effect of Bacillus Complex on Water Quality and Bacterial Community Structure of Carassius aumtus Culture Water [J]. Journal of Ecology and Rural Environment, 2020, 36(4): 522-530.
[3]	HONG Xin, HAN Cheng, KONG Fan, ZHOU Feng-wu, WU Shao-song, ZHONG Wen-hui, LIU Biao. Effects of Insect-resistant and Herbicide-tolerant Transgenic Maize on Rhizospheric Bacterial and Fungal Communities [J]. Journal of Ecology and Rural Environment, 2020, 36(3): 358-366.
[4]	XU Xia, LIU Fei, XUE Yin-gang, TU Bo-wen, ZHOU Lu-lu, JIANG Xiao-dong, SHI Xin-lan, XUE Ke. Bacterial Community Structure and Its Influence Factors of the Groundwater in an Industry Park [J]. Journal of Ecology and Rural Environment, 2019, 35(2): 255-263.
[5]	XUE Yin-gang, JIANG Xiao-dong, SUN Meng, LIU Fei, TENG Jia-quan, GENG Jin-ju, XIE Wen-li, ZHANG Hao. Structure and Diversity Profiles of Planktonic and Sediment Bacteria Communities in the Zhushan Bay of Lake Taihu in Winter Based on High-Throughput Sequencing [J]. Journal of Ecology and Rural Environment, 2017, 33(11): 992-1000.
[6]	CAO Hong-lei, YAN Ting-mei, QIAO Jun, ZHU Ning-yuan. Comprative Study on Effect of Dicyandiamide Inhibiting Nitrifcation in Fluvo-Aquic Soil and Lime Concretion Black Soil [J]. Journal of Ecology and Rural Environment, 2016, 32(1): 110-114.
[7]	WANG Zhi-gang,WANG Zhi-gang,WANG Zhi-gang,HU Yun-long,XU Wei-hui,MO Ji-xian,LI Shan-shan,ZHANG Zhi,YANG Zhi-hong. Impacts of Dimethyl Phthalate Contamination on Abundance and Diversity of Microbes in Black Soil. [J]. Journal of Ecology and Rural Environment, 2015, 31(5): 779-783.
[8]	LI Wei, QIAO Yu-Qiang, CHEN Huan, DU Shi-Zhou, ZHAO Zhu, CAO Cheng-Fu. Soil Organic Matter Composition and Carbon Pool Management Index in Shajiang Black Soil as Affected by Straw Incorporation Coupled With Fertilization [J]. Journal of Ecology and Rural Environment, 2014, 30(4): 475-480.
[9]	JIANG Heng, ZOU Wen-Xiu, HAN Xiao-Zeng, YANG Chun-Bao, HAO Xiang-Xiang, LI Meng. Effects of Land Use and Fertilization on Physical Properties of Black Soil [J]. Journal of Ecology and Rural Environment, 2013, 29(5): 599-604.
[10]	ZOU Wen-Xiu, HAN Xiao-Zeng, WANG Shou-Yu, JIANG Heng, YANG Chun-Bao. Water consumption by Maize Under Different Fertilization Managements in Black Soil Zone of Northeast China [J]. Journal of Ecology and Rural Environment, 2012, 28(6): 681-686.
[11]	HUANG Jing, ZHANG Yang-Zhu, LIU Hong-Bin, WANG Bo-Ren. CO₂ and N₂O Emissions From Red Soil During Wheat and Corn Growing Seasons Under Different Ptterns of Long-Term Fertilization [J]. Journal of Ecology and Rural Environment, 2011, 27(4): 7-13.
[12]	ZHANG Wei, HE Hong-Bo, JIE Hong-Tu, BAI Zhen, ZHANG Xu-Dong. Effect of Glucose on Mineralization of Organic Nitrogen in Black Soil,Northeast of China [J]. Journal of Ecology and Rural Environment, 2009, 25(4): 52-54.
[13]	ZOU Wen-Xiu, HAN Xiao-Zeng, LI Liang-Hao, WANG Shou-Yu, WANG Feng-Xian. Effect of Water Treatment on Water Regime in Farmland Black Soil [J]. Journal of Ecology and Rural Environment, 2009, 25(3): 35-38.
[14]	ZHANG Di, HAN Xiao-Zeng, LI Hai-Bo, SONG Chun, HOU Xue-Ying. Impacts of Long-Term Vegetation and Fertilization Management on Black Soil Labile Organic Carbon and Carbon Management Index [J]. Journal of Ecology and Rural Environment, 2008, 24(4): 1-5.
[15]	JIN Liang, LI Lian-Qing, PAN Gen-Xing, WU Xin-Min, LIAO Qi-Lin. Distribution of Heavy Metals in the Soil-Rice System and Food Exposure Risk Assessment of North Jiangsu,China [J]. Journal of Ecology and Rural Environment, 2007, 23(1): 33-39.