还原态硫介导沉积物-水系统硝酸盐还原过程研究

doi:10.19741/j.issn.1673-4831.2021.0172

Abstract

Abstract: There is a coupling of sulfur cycle with denitrification (DN) and dissimilatory nitrate reduction to ammonium (DNRA) in the sediment-water system. The effects of reduced sulfur on DN and DNRA were revealed by sequentially adding 0 (Group N), 50 (Group L) and 75 mg·L^-1 (Group H) reduced sulfur to the constructed sediment-water microcosm systems, and then the concentration changes of various forms of nitrogen in overlying water, pore water and sediment as well as microbial community structure were analyzed. Results show that 38.02% and 33.27% of reduced sulfur were converted into SO₄^2-, respectively, when the dosage of reduced sulfur was 50 and 75 mg·L^-1. Sulfur oxidation promoted the occurrence of DN, and caused the total nitrogen of Group L and Group H decrease by 11% and 8%, respectively, compared with Group N, but had no significant effect on the process of DNRA. In addition, the sum of relative abundances of genera Thiobacillus and Dechloromonas functioned with sulfur autotrophic denitrification increased with concentration of reduced sulfur, and occupied 5.69%, 6.52%, 8.70% in Group N, L and H, respectively. In contrast, the relative abundance of heterotrophic nitrate reducing bacteria (HNRB) was only 0.98%, 0.91%, and 1.05% in Group N, L and H, respectively, and the DNRA bacteria (with the relative abundance no more than 1%) were unaffected by reduced sulfur. Such results further confirmed that the nitrate reduction process in the system was dominated by sulfur autotrophic denitrification.

Key words: reduced sulfur, denitrification, dissimilatory nitrate reduction to ammonium, nitrate reduction

CLC Number:

X524
X172

CHEN Yu-hua, LU Rui, RONG Peng, WANG Pei, WU Zhen-bin, XIAO En-rong. Study on Reduction of Nitrate in Sediment-water System Mediated by Reduced Sulfur[J]. Journal of Ecology and Rural Environment, 2022, 38(3): 350-357.

References

[1] KUYPERS M M M,MARCHANT H K,KARTAL B.The Microbial Nitrogen-cycling Network[J].Nature Reviews Microbiology,2018,16(5):263-276.
[2] ZHU J,HE Y,ZHU Y S,et al.Biogeochemical Sulfur Cycling Coupling with Dissimilatory Nitrate Reduction Processes in Freshwater Sediments[J].Environmental Reviews,2018,26(2):121-132.
[3] 李文超,王文浩,何岩,等.黑臭河道沉积物中硫铁行为与氮磷循环的耦合机制[J].华东师范大学学报(自然科学版),2015(2):1-8,29.[LI Wen-chao,WANG Wen-hao,HE Yan,et al.Coupling of Sulfur and Iron with Nitrogen and Phosphorus in Sediments of Malodorous River:A Review[J].Journal of East China Normal University (Natural Science),2015(2):1-8,29.]
[4] MURPHY A E,BULSECO A N,ACKERMAN R,et al.Sulphide Addition Favours Respiratory Ammonification (DNRA) over Complete Denitrification and Alters the Active Microbial Community in Salt Marsh Sediments[J].Environmental Microbiology,2020,22(6):2124-2139.
[5] PANG Y M,WANG J L,LI S J,et al.Long-term Sulfide Input Enhances Chemoautotrophic Denitrification rather than DNRA in Freshwater Lake Sediments[J].Environmental Pollution,2021,270:116201.
[6] AELION C M,WARTTINGER U.Low Sulfide Concentrations Affect Nitrate Transformations in Freshwater and Saline Coastal Retention Pond Sediments[J].Soil Biology and Biochemistry,2009,41(4):735-741.
[7] YANG X N,HUANG S,WU Q H,et al.Nitrate Reduction Coupled with Microbial Oxidation of Sulfide in River Sediment[J].Journal of Soils and Sediments,2012,12(9):1435-1444.
[8] LU W W,RIYA S,ZHOU S,et al.In Situ Dissimilatory Nitrate Reduction to Ammonium in a Paddy Soil Fertilized with Liquid Cattle Waste[J].Pedosphere,2012,22(3):314-321.
[9] BRUNET R C,GARCIA-GIL L J.Sulfide-induced Dissimilatory Nitrate Reduction to Ammonia in Anaerobic Freshwater Sediments[J].FEMS Microbiology Ecology,1996,21(2):131-138.
[10] LU W W,ZHANG H L,SHI W M.Dissimilatory Nitrate Reduction to Ammonium in an Anaerobic Agricultural Soil as Affected by Glucose and Free Sulfide[J].European Journal of Soil Biology,2013,58:98-104.
[11] 辛明秀,赵颖,周军,等.反硝化细菌在污水脱氮中的作用[J].微生物学通报,2007,34(4):773-776.[XIN Ming-xiu,ZHAO Ying,ZHOU Jun,et al.The Application of Denitrifying Bacteria in Denitrification of Wastewater[J].Microbiology,2007,34(4):773-776.]
[12] 左宇.硫自养生物反硝化过程N₂O排放特征与机制研究[D].北京:北京化工大学,2018.[ZUO Yu.Characteristics and Mechanism of N₂O Emission from Sulfur Autotrophic Denitrification[D].Beijing:Beijing University of Chemical Technology,2018.]
[13] DI CAPUA F,AHORANTA S H,PAPIRIO S,et al.Impacts of Sulfur Source and Temperature on Sulfur-driven Denitrification by Pure and Mixed Cultures of Thiobacillus[J].Process Biochemistry,2016,51(10):1576-1584.
[14] XU D,XIAO E R,XU P,et al.Performance and Microbial Communities of Completely Autotrophic Denitrification in a Bioelectrochemically-assisted Constructed Wetland System for Nitrate Removal[J].Bioresource Technology,2017,228:39-46.
[15] TAN X,YANG Y L,LI X,et al.Intensified Nitrogen Removal by Heterotrophic Nitrification Aerobic Denitrification Bacteria in Two Pilot-scale Tidal Flow Constructed Wetlands:Influence of Influent C/N Ratios and Tidal Strategies[J].Bioresource Technology,2020,302:122803.
[16] 张亚平,万宇,聂青,等.湖泊水体中氮的生物地球化学过程及其生态学意义[J].南京大学学报(自然科学),2016,52(1):5-15.[ZHANG Ya-ping,WAN Yu,NIE Qing,et al.Biogeochemical Processes of Nitrogen in Lake Systems and Their Ecological Importances[J].Journal of Nanjing University (Natural Sciences),2016,52(1):5-15.]
[17] XU P,XIAO E R,XU D,et al.Internal Nitrogen Removal from Sediments by the Hybrid System of Microbial Fuel Cells and Submerged Aquatic Plants[J].PLoS One,2017,12(2):e0172757.
[18] 姚莉.湖泊氮循环研究进展[J].广东化工,2020,47(10):112-113,100.[YAO Li.Research Progress of Nitrogen Cycle in Lakes[J].Guangdong Chemical Industry,2020,47(10):112-113,100.]
[19] 焦立新.浅水湖泊表层沉积物氮形态特征及在生物地球化学循环中的功能[D].呼和浩特:内蒙古农业大学,2007.[JIAO Li-xin.Nitrogen Forms Characteristic in the Sediments from the Shallow Lakes and Functions in Biogeochemical Cycling[D].Hohhot:Inner Mongolia Agricultural University,2007.]
[20] 曾巾,杨柳燕,肖琳,等.湖泊氮素生物地球化学循环及微生物的作用[J].湖泊科学,2007,19(4):382-389.[ZENG Jin,YANG Liu-yan,XIAO Lin,et al.Biogeochemical Cycling of Nitrogen in Lakes and the Role of Microorganisms in Conversion of Nitrogen Compounds[J].Journal of Lake Sciences,2007,19(4):382-389.]
[21] BURGIN A J,HAMILTON S K,JONES S E,et al.Denitrification by Sulfur-oxidizing Bacteria in a Eutrophic Lake[J].Aquatic Microbial Ecology,2012,66(3):283-293.
[22] 李生来.硫化物对河道底泥反硝化脱氮效能的影响[J].中国农村水利水电,2019(10):42-46.[LI Sheng-lai.The Effect of Sulfide on Denitrification and Denitrification Efficiency of River Sediments[J].China Rural Water and Hydropower,2019(10):42-46.]
[23] 李南锟,杜帅,刘莉,等.葡萄糖对硫自养反硝化性能及微生物群落的影响[J].环境科学与技术,2019,42(12):8-13.[LI Nan-kun,DU Shuai,LIU Li,et al.Effects of Glucose on Nitrogen Removal Performance and Microbial Community in Sulfur Autotrophic Denitrification System[J].Environmental Science & Technology,2019,42(12):8-13.]
[24] 寄博华,李玮,常军军,等.滇池湖滨湿地不同挺水植物区沉积物细菌群落结构特征[J].生态与农村环境学报,2020,36(3):390-398.[JI Bo-hua,LI Wei,CHANG Jun-jun,et al.Characteristics of Bacterial Community Structure in Sediments with Different Emergent Plants in Dianchi Lakeside Wetland[J].Journal of Ecology and Rural Environment,2020,36(3):390-398.]
[25] SONG J,LI Q,DZAKPASU M,et al.Integrating Stereo-elastic Packing into Ecological Floating Bed for Enhanced Denitrification in Landscape Water[J].Bioresource Technology,2020,299:122601.
[26] ZHOU L J,OU P X,ZHAO B K,et al.Assimilatory and Dissimilatory Sulfate Reduction in the Bacterial Diversity of Biofoulant from a Full-scale Biofilm-membrane Bioreactor for Textile Wastewater Treatment[J].Science of the Total Environment,2021,772:145464.
[27] BURGIN A J,HAMILTON S K.NO₃^--driven SO₄^2- Production in Freshwater Ecosystems:Implications for N and S Cycling[J].Ecosystems,2008,11(6):908-922.
[28] 李杨.不同电子供体的硫自养反硝化脱氮效能比较与脱氮机制研究[D].上海:上海交通大学,2017.[LI Yang.A Comparative Study on Sulfur-based Autotrophic Denitrification and Its Mechanisms[D].Shanghai:Shanghai Jiaotong University,2017.]
[29] SHAO M F,ZHANG T,FANG H H P.Sulfur-driven Autotrophic Denitrification:Diversity,Biochemistry,and Engineering Applications[J].Applied Microbiology and Biotechnology,2010,88(5):1027-1042.
[30] 王元涛,王晓玲,刘时光,等.人工湿地沟壕系统中硝酸盐异化还原成铵细菌群落特征研究[J].环境科学学报,2020,40(6):2175-2185.[WANG Yuan-tao,WANG Xiao-ling,LIU Shi-guang,et al.Community Characteristics of Dissimilatory Nitrate Reduction to Ammonium Bacteria in Ditch System of Constructed Wetland[J].Acta Scientiae Circumstantiae,2020,40(6):2175-2185.]

Study on Reduction of Nitrate in Sediment-water System Mediated by Reduced Sulfur

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments

[1]	SHI Zhen-qi, SHE Dong-li, CHEN Xin-yi, XIA Yong-qiu. Effects of Salinity on Soil Ammonia Volatilization and Denitrification Rates [J]. Journal of Ecology and Rural Environment, 2021, 37(11): 1458-1464.
[2]	ZHOU Guang-ji, ZHANG Lan-he, YE Zhen-qi, XU Yan, WEI Lai, FANG Guo-cheng. Effect of Air-Water Ratio on Reclaimed Water Treatment in a Biological Aerated Filter [J]. Journal of Ecology and Rural Environment, 2019, 35(2): 264-271.
[3]	WU Jin-bo, ZHANG Wu-ping, WANG Guo-fang, BU Yu-shan, JIA Ruo-nan, ZHANG Xi, ZHANG Xiao-hong. Life Cycle Assessment of the Maize Production Under Different Water Conditions [J]. Journal of Ecology and Rural Environment, 2019, 35(11): 1396-1403.
[4]	YU Lin, WANG Dong-Qi, DENG Huan-Guang, LI Yang-Jie, CHANG Si-Qi, YE Xiao, CHEN Zhen-Lou. Denitrification Rates in Sediments of Suzhou River and Rivers in Chongming Island of Shanghai and Their Affecting Factors [J]. Journal of Ecology and Rural Environment, 2015, 31(1): 100-105.
[5]	ZHANG Li, ZHANG Zhen-Hua, GAO Yan, YAN Shao-Hua. Effect of Aquatic Plants on Emission of Gases From Eutrophic Water [J]. Journal of Ecology and Rural Environment, 2014, 30(6): 736-743.
[6]	LI Hui-Zhen, WU Sheng-Fa, HUA Gui-Fen, CHEN Jin-Yuan. Denitriding Characteristic of A Heterotrophic Nitrifying Strain of Bacteria and Its Preliminary Application [J]. Journal of Ecology and Rural Environment, 2014, 30(3): 352-357.
[7]	ZHENG Mei-Ling, Lü Xi-Wu , WANG Yong-Qian. Effect of Internal Recycle Ratio on Denitrification and Deodorization Eeffects of the Bio-Ecological Technology [J]. Journal of Ecology and Rural Environment, 2013, 29(5): 630-634.
[8]	YANG Jing-Chao, XIA Xun-Feng, XI Bei-Dou, QIU Hong-Jiang, WANG Lei, SU Yi, XIA Tian-Jiao. Effect of Aeration/Non-Aeration Ratio on Anearobically Digested Pig Farm Wastewater Treated in Two-stage Sequencing Batch Biofilm Reactor [J]. Journal of Ecology and Rural Environment, 2013, 29(2): 248-252.
[9]	HE Wei, WANG Wei, WANG Jie, SONG Ming-Liang, ZHONG Wen-Hui. Isolation,Identification and Its Mixed Application of A Strain of Aerobic Denitrifying Bacteria [J]. Journal of Ecology and Rural Environment, 2009, 25(2): 88-93.
[10]	ZENG Jin, YANG Liu-Yan, XIAO Lin, DU Hong-Wei. Comparative Study on Transformation Potential of Dissolved Inorganic Nitrogen in Different Parts of Lake Taihu [J]. Journal of Ecology and Rural Environment, 2008, 24(1): 63-67.
[11]	HAN Bing, WANG Xiao-Ke, OU Yang-Zhi-Yun . Saturation levels and carbon sequestration potentials of soil carbon pools in farmland ecosystems of China [J]. Journal of Ecology and Rural Environment, 2005, 21(4): 6-11.
[12]	FU Li-Jian, GUO Dan-Zhao, SHI Chun-Long, HUANG Wei-Yi. Effect of carbon source and C/N ratio on heterotrophic denitrification of pure culture [J]. Journal of Ecology and Rural Environment, 2005, 21(2): 42-45.
[13]	PU He-Chun, LIU Guang-Shen, YUAN Zhi-Yun, HONG Ye-Tang. Relationship of denitrification coupled to nitrification in soil-water system [J]. Journal of Ecology and Rural Environment, 1995, 11(3): 19-22.