基于微生物化学计量学研究有机肥促进稻田土壤磷转化的作用

doi:10.19741/j.issn.1673-4831.2022.0430

Abstract

Abstract: Organic fertilizers significantly increases soil phosphorus (P) pools in paddy soils. Microbial stoichiometry can play an important role in the process of reflecting soil P transformation. Based on 100% replacement of chemical fertilizers with organic fertilizers at different nitrogen application rates, including 0 (N₀), 75 (N₇₅), 150 (N₁₅₀), 225 (N₂₂₅) and 300 kg·hm^-2 (N₃₀₀), this study analyzed the effect of organic fertilizers on soil P pools in paddy fields and the effect of microbial stoichiometry on soil P transformation in different rice-growing stages. The results show that in the third year, compared with no fertilizer treatment, all organic fertilizer treatments increased the concentration of soil Olsen-P by 12.9%-153% and TP by 5.80%-40.6%), respectively, at rice seedling stage and harvest stage. Further analysis of the soil P components changes showed that the available P(Resin-P, NaHCO₃-Pi, NaHCO₃-Po), secondary-P(NaOH-Pi, NaOH-Po), and organic P (Po)(Labile Organic P, Moderately Labile Organic P, Stable Organic P) increased by 37.3%-142%, 7.50%-41.3% and 3.41%-32.2%, respectively. With the further increase of organic fertilizer application (N₂₂₅ and N₃₀₀), soil labile P (including Olsen-P and available P) did not increase. Through Redundancy Analysis and Pearson Correlation Analysis, it was found that soil MBC and MBN were significantly negatively correlated with soil Olsen-P, available P, secondary-P and labile organic P (LPo), indicating that the increase of microbial biomass decreased the soil labile P concentration. However, MBP was significantly positively correlated with soil Olsen-P and soil inorganic P (Pi) components at harvest stage, because rice roots changed the rhizosphere environment, which in turn affected soil microbial biomass and soil Pi. During the growth period of rice seedling season, the microbial stoichiometric ratio (MBC/MBP and MBN/MBP) was significantly negatively correlated with soil LPo, while in the rice harvest stage, the microbial stoichiometric ratio (MBC/MBP and MBN/MBP) was significantly negatively correlated with soil Olsen-P and Pi. It showed that microbial stoichiometry significantly affected soil labile P and Po, and promoted the conversion of soil Po to labile P. This study aims to provide a theoretical basis for guiding the application of organic fertilizers to promote P in paddy soil and increase its efficiency.

Key words: phosphorus, organic fertilizer, paddy soil, labile P, microbial stoichiometry

CLC Number:

S158

ZHANG Ling-yu, CHEN Guang-lei, ZHAO Hong-meng, WU Li-wu, CHEN Hao, YU Yun-fei, YE Wen-ling, WANG Yu. Effects of Organic Fertilization on Soil Phosphorus Transformation in Paddy Field by Using Microbial Stoichiometry[J]. Journal of Ecology and Rural Environment, 2023, 39(12): 1610-1618.

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Effects of Organic Fertilization on Soil Phosphorus Transformation in Paddy Field by Using Microbial Stoichiometry

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