Journal of Ecology and Rural Environment ›› 2001, Vol. 17 ›› Issue (2): 30-34.doi:

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Effect of Cd2+ on Antioxidase System and Ultrastructure of Ottelia alismoides (L.)Pers. Leaves

XU  Qin-Song, SHI  Guo-Xin, DU  Kai-He   

  1. College of Life Science,Nanjing Normal University
  • Received:2000-09-20 Online:2001-05-10 Published:2011-11-22

Abstract: Investigation was made of effect of simulated Cd 2+ pollution on the antioxidase system and ultrastructure of the cells of Ottelia alismoides(L.)Pers.leaves,mainly chloroplasts,mitochondria and cell nucleus. It was found that with increase in the stress,superoxide dismutase(SOD)activity and soluble protein content decreased drastically,while malonyl(MDA)increased;and that the activity of peroxidase(POD)and catalase(CAT)peaked at 0 1mg/L of the pollutant and declined afterwards. Electronic microscopic observation showed that at the early stage of the toxication,chloroplasts and mitochondria were swelling up;and thylakoids vacuolized apparently. Higher concentration of Cd 2+ aggravated the damage,making chloroplast membranes disappear and chloroplasts disintegrate,mitochondrion mesenchyme dissolve and mitochondria be on the verge of disorganization,and nucleolus chromatin agglutinate and merge into cytoplasm. Cd 2+ pollution not only destroys the plant’s antioxidase system,but also causes irreversible damage to its ultrastructure,thus leading the cells to death. 

Key words: antioxidase system, Cd2+, Ottelia alismoides(L.)Pers., stress, ultrastructure

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