Journal of Ecology and Rural Environment ›› 2003, Vol. 19 ›› Issue (3): 26-30.doi:
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CHEN Guo-Xiang, ZHANG Rong-Xi, HE Bing, WEI Jin-Cheng
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Abstract: Effects of light stress on function and structure characteristics of photosynthetic membranes (thylakoid membranes and PS Ⅱ particles) in leaves of wheat( Triticum aestivum L.cv.Yangmai No.5)seedling were studied by measuring changes in O 2 evolving activity,fluorescence emission spectra and membrane protein polypeptide components. Results showed that light stress (2 500 μmol·m -2 ·s -1 ) induced decline of electron transport activities of thylakoid membranes and PS Ⅱ particles and decrease in fluorescence emission intensities. When PS Ⅱ particles in wheat leaves were illuminated with higher light intensity (2 500 μmol· m -2 ·s -1 ) at 25 ℃, the extrinsic protein (OEC 33 000,23 000,17 000 u) and the chlorophyll binding protein CP43 of PS Ⅱ complex were degraded. It seems that OEC 33 000 u polypeptide plays a role in regulating stabilization of the PS Ⅱ reaction center structure under light stress.
Key words: Triticum aestivum L cv Yangmai No.5, PS Ⅱ particle, light stress, O 2 evolving activity, fluorescence emission intensity, polypeptide component
CLC Number:
S512.1
CHEN Guo-Xiang, ZHANG Rong-Xi, HE Bing, WEI Jin-Cheng. Effect of light stress on structure and function of PS Ⅱ in wheat(Triticum aestivum L.cv.Yangmai No.5)seedling leaves[J]. Journal of Ecology and Rural Environment, 2003, 19(3): 26-30.
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