Journal of Ecology and Rural Environment ›› 2009, Vol. 25 ›› Issue (3): 69-72.doi: CNKI:SUN:NCST.0.2009-03-014

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Isolation and Identification of a Strain of Cypermethrin-Degrading Bacteria LQ-3 and Its Degradation Characteristics

ZHANG  Li-Ping, XU  Lian, WU  Ying, LIN  Dong-Qing, LIU  Xin, SHEN  Biao   

  1. College of Life Science,Nanjing Agricultural University
  • Received:2008-12-02 Online:2009-07-25 Published:2011-04-08
  • Contact: SHEN Biao College of Life Science,Nanjing Agricultural University E-mail:shenbiao@njau.edu.cn

Abstract: A strain of cypermethrin-degrading bacteria referred to as LQ-3 was isolated from sludge collected from a wastewater outlet of a pesticide plant.Based on morphological,physiological and biochemical characteristics and 16S rDNA sequences analysis(GenBank Accession No.FJ222585),LQ-3 was identified as Starkeya sp..LQ-3 degraded cypermethrin through co-metabolism.When fed with nutrient substances,such as yeast extract,peptone and glucose,LQ-3 could degrade 72.1% of cypermethrin in the medium(20 mg·L-1) in 5 d.The optimal pH and temperature for the degradation by LQ-3 was 7-8 and 30 ℃,respectively.Strain LQ-3 was also able to degrade other pyrethroids such as cyhalothrin,fenpropathrin,bifenthrin and deltamethrin besides cypermethrin.The enzyme distribution experiment showed that the enzyme responsible for the cypermethrin degradation was extracellular.

Key words: cypermethrin, biodegradation, Starkeya sp.LQ-3, degradation character, enzyme distribution

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