Abstract: Samples of different kinds of animal and poultry manures and of soils from typical vegetable fields and orchards of Hangzhou area that had been applied with these manures, were collected separately for analysis of concentrations of heavy metals and pollution of 14 selected antibiotics to study the effects of long-term application of the manures on distribution of heavy metals and antibiotics in soil profiles of these fields.Results show that of the three kinds of livestock and poultry manures, pig manure was the most liable to soil pollution, for it contained 197.0 mg•kg^-1 of Cu, 947.0 mg•kg^-1 of Zn and 1.35 mg•kg^-1 of Cd. Among the lands under different patterns of land use, greenhouse vegetable lands were the most prominent in having heavy metals accumulated in the surface soil, especially Zn and Cd, being 203 and 0.48 mg•kg^-1, respectively, in the 0-20cm soil layer. The 14 selected antibiotics, including tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), doxycycline (DXC), sulfadiazine (SD), sulfamethoxazole (SMZ), sulfamethazine (SMX), norfloxacin (NFC), ofloxacin (OFC), enythromycin-H₂O (ETM-H₂O), roxithromycin (RTM), chloramphenicol (CPC), thiamphenical (TPC) and florfenicol (FFC), varied significantly in concentration and distribution in soil profiles with land use pattern. They decreased rapidly in concentration with soil depth, but some of them were still detectable in the 80 -100 cm soil layer. Compared to orchards, the vegetable lands had more antibiotics accumulated in the surface soil layer as residue, which was 39.5 μg•kg^-1 in concentration and composed mainly of TCs and fluoroquinolones antibiotics, reaching 34.3 and 4.75μg•kg^-1, respectively. Obviously, long-term application of livestock manure in farmland might result in the combined pollution of heavy metals and antibiotics, and is apparently a potential ecological risk.

Key words: livestock and poultry manure, vegetable garden, orchard, soil profile, heavy metals, antibiotics