武汉机器荡子湖蓝藻水华优势藻种和土嗅味藻源鉴定

doi:10.11934/j.issn.1673-4831.2017.06.008

Abstract

Abstract:

Research indicates cyanobacteria blooms not only endanger water quality,but also produce neurotoxins and odorous matter. In order to scientifically predict water bloom, and then provide fundamental knowledge on the prevention and control of water bloom and odor, preponderant algae species of cyanobacteria bloom and geosmin odor source multiplied in early spring were identified by means of PCR amplification and gene sequencing technology. The effects of different temperature and light intensity on preponderant algae biomass and geosimin odor yield were also measured respectively under laboratory conditions. The preponderant algae were cultured at three temperatures (16, 26 and 36℃) and three light intensities[16, 26, 36 μmol·m^-2·s^-1]. The preponderant algae of cyanobacteria bloom was identified as Aphanizomenon gracile, and the strong smell of the eutrophicated lake might come from the geosmin of Aphanizomenon gracile. Laboratory simulation study indicate that the content of Aphanizomenon gracile chlorophyll-a and the yield of geosmin odor became higher at low temperature and high light intensity, indicating low temperature and strong light environment were favorable growth conditions. It was found that the effect of temperature was more significant than light intensity on Aphanizomenon gracile, and the highest yield of the geosmin odor was 6 555 ng·mg^-1 under favorable conditions.

Key words: cyanobacteria bloom, gene sequencing, PCR amplification, geosmin odor

CLC Number:

X171

WAN Hong, XU Wan-wan. Identification on Preponderant Algae of Cyanobacteria Bloom and Geosmin Odor Source of Wuhan Jiqidangzi Lake[J]. Journal of Ecology and Rural Environment, 2017, 33(6): 539-545.

References

[1] JØRGENSEN S E.State-of-the-Art of Ecological Modelling With Emphasis on Development of Structural Dynamic Models[J].Ecological Modelling,1999,120(2/3):75-96.
[2] 国家环境保护总局.2007中国环境状况公报[R].北京:国家环境保护总局,2008:6.[State Environmental Protection Administration.The State of Environment in China in 2007[R].Beijing:State Environmental Protection Administration,2008:6.]
[3] 金根东.我国湖泊富营养化研究现状[J].现代农业科技,2008(16):334-336.[JIN Gen-dong.Research Status of Lake Eutrophication in China[J].Modern Agricultural Science and Technology,2008(16):334-336.]
[4] 黄睿,沈烽,罗娟,等.蓝藻水华消亡对湖泊表层沉积物中氨氧化细菌丰度和群落结构的影响[J].生态与农村环境学报,2015,31(3):334-339.[HUANG Rui,SHEN Feng,LUO Juan,et al.Effects of Withering of Cyanobacteria Bloom on Abundance and Community Composition of Ammonia-Oxidizing Bacteria in Surface Lake Sediments[J].Journal of Ecology and Rural Environment,2015,31(3):334-339.]
[5] 钱奎梅,刘霞,段明,等.鄱阳湖蓝藻分布及其影响因素分析[J].中国环境科学,2016,36(1):261-267.[QIAN Kui-mei,LIU Xia,DUAN Ming,et al.Distribution and Its Influencing Factors of Bloom-Forming Cyanobacteria in Poyang Lake[J].China Environmental Science,2016,36(1):261-267.]
[6] 黄炜,商兆堂.蓝藻水华轻度的显著相关环境因素识别模型[J].生态与农村环境学报,2013,29(6):804-810.[HUANG Wei,SHANG Zhao-tang.Model for Identifying Environmental Factors Significantly Associated With Cyanobacterial Bloom Intensity[J].Journal of Ecology and Rural Environment,2013,29(6):804-810.]
[7] 范裕祥,金社军,周培,等.巢湖蓝藻水华分布特征和气象条件分析[J].安徽农业科学,2015,43(4):19l-193,198.[FAN Yu-xiang,JIN She-jun,ZHOU Pei,et al.Analysis on the Distributions and Meteorological Conditions of Cyanobacteria Bloom in Chaohu Lake[J].Journal of Anhui Agricultural Sciences,2015,43(4):19l-193,198.]
[8] NEILAN B A,PEARSON L A,MUENCHHOFF J,et al.Environmental Conditions That Influence Toxin Biosynthesis in Cyanobacteria[J].Environmental Microbiology,2012,15(5):1239-1253.
[9] PAERL H W,HALL N S,CALANDRINO E S.Controlling Harmful Cyanobacterial Blooms in a World Experiencing Anthropogenic and Climatic-Induced Change[J].Science of the Total Environment,2011,409(10):1739-1745.
[10] BALLOT A,FASTNER J,WIEDNER C.Paralytic Shellfish Poisoning Toxin-Producing Cyanobacterium Aphanizomenon gracilein Northeast Germany[J].Applied and Environmental Microbiology,2010,76(4):1173-1180.
[11] 邓绪伟,陶敏,张路,等.洞庭湖水体异味物质及其与藻类和水质的关系[J].环境科学研究,2013,26(1):16-21.[DENG Xu-wei,TAO Min,ZHANG Lu,et al.Relationships Between Odors and Algae and Water Quality in Dongting Lake[J].Research of Environmental Sciences,2013,26(1):16-21.]
[12] PETER A,KÖSTER O,SCHILDKNECHT A,et al.Occurrence of Dissolved and Particle-Bound Taste and Odor Compounds in Swiss Lake Waters[J].Water Research,2009,43(8):2191-2200.
[13] SAADOUN I M K,SCHRADER K K,BLEVINS W,et al.Environmental and Nutritional Factors Affecting Geosmin Synthesis by Anabaena sp.[J].Water Research,2001,35(5):1209-1218.
[14] GERBER N N,LECHEVALIER H A.Geosmin,an Earthy-Smelling Substance Isolated From Actinomycetes[J].Applied Microbiology,1965,13(6):935-938.
[15] LAWTON L A,ROBERTSON P K J,ROBERTSON R F,et al.The Destruction of 2-Methylisobomeol and Geosmin Using Titanium Dioxide Photocatalysis[J].Applied Catalysis B:Environmental,2003,44(1):9-13.
[16] SCHULZ S,FUHLENDORFF J,REICHENBACH H.Identification and Synthesis of Volatiles Released by the Myxobacterium Chondromyces crocatus[J].Tetrahedron,2004,60(17):3863-3872.
[17] 施军琼,吴忠兴,马剑敏,等.水华束丝藻对磷的生理响应研究[J].水生生物学报,2011,35(5):857-861.[SHI Jun-qiong,WU Zhong-xing,MA Jian-min,et al.Physiological Response to Phosphorus in Aphanizomenon flos-aquae[J].Acta Hydrobiological Sinica,2011,35(5):857-861.]
[18] 马健荣,邓建明,秦伯强,等.湖泊蓝藻水华发生机理研究进展[J].生态学报,2011,33(10):3020-3030.[MA Jian-rong,DENG Jian-ming,QIN Bo-qiang,et al.Progress and Prospects on Cyanobacteria Bloom-Forming Mechanism in Lakes[J].Acta Ecologica Sinica,2011,33(10):3020-3030.]
[19] 朱英,陶刚,刘作易,等.琼脂糖凝胶电泳操作中值得注意的几个问题[J].贵州农业科学,2004,32(6):27-28.[ZHU Ying,TAO Gang,LIU Zuo-yi,et al.Several Problems Worthy of Being Noticed During Agarose Gel Electrophoresis[J].Guizhou Agricultural Sciences,2004,32(6):27-28.]
[20] 彭加平,田锦.基因操作技术[M].北京:化学工出版社,2013:44-47.[PENG Jia-ping,TIAN Jin.Gene Manipulation Technology[M].Beijing:Chemical Industry Press,2013:44-47.]
[21] 钟卫鸿.基因工程技术实验指导[M].北京:化学工出版社,2007:45-56.[ZHONG Wei-hong.Experimental Guidance of Genetic Engineering Technology[M].Beijing:Chemical Industry Press,2007:45-56.]
[22] WATSON S B,BROWNLEE B,SATCHWILL T,et al.Quantitative Analysis of Trace Levels of Geosmin and Mib in Source and Drinking Water Using Headspace SPME[J].Water Research,2000,34(10):2818-2828.
[23] LI L,WAN N,GAN N Q,et al.Annual Dynamics and Origins of the Odorous Compounds in the Pilot Experimental Area of Lake Dianchi,China[J].Water Science and Technology,2007,55(5):43-50.
[24] 马杰,方赤光,李青,等.气相色谱-质谱联用法测定生活饮用水中的土臭素和2-甲基异莰醇[J].中国卫生检验杂志,2016,26(1):23-25.[MA Jie,FANG Chi-guang,LI Qing,et al.Determination of Geosmin and 2-Methylisoborneol in Drinking Water by Gas Chromatography-Mass Spectrometry[J].Chinese Journal of Health Laboratory Technology,2016,26(1):23-25.]
[25] TSUJIMURA S,ISHIKAWA K,TSUKADA H.Effect of Temperature on Growth of the Cyanobacterium Aphanizomenon flosaquaein Lake Biwa and Lake Yogo[J].Phycological Research,2001,49(4):275-280.
[26] CARMICHAEL W W,DRAPEAU C,ANDERSON D M.Harvesting of Aphanizomenon flos-aquae Ralfs Ex Born. and & Flah. var. Flos-aquae(Cyanobacteria) From Klamath Lake for Human Dietary Use[J].Journal of Applied Phycology,2000,12(6):585-595.
[27] NAES H,AARNES H,UTKILEN H,et al.Effect of Photon Fluence Rate and Specific Growth Rate on Geosmin Production of the Cyanobacterium Oscillatoria brevis (Kütz.) Go[J].Applied and Environmental Microbiology,1985,49(6):1538-1540.
[28] BLEVINS W T,SCHRADER K K,SAADOUN I.Comparative Physiology of Geosmin Production by Streptomyces halstedii and Anabaena sp.[J].Water Science and Technology,1995,31(11):127-133.

Identification on Preponderant Algae of Cyanobacteria Bloom and Geosmin Odor Source of Wuhan Jiqidangzi Lake

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