Journal of Ecology and Rural Environment ›› 2009, Vol. 25 ›› Issue (1): 73-78.doi: CNKI:SUN:NCST.0.2009-01-014
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CAO Juan, XU Zhi-Hui, LI Ling-Zhi, SHEN Biao
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Abstract: A strain of biosurfactant-producing petroleum-degrader-BHSN was isolated from petroleum-contaminated soil.Strain BHSN was identified as Acinetobacter sp.according to its morphological,physiological,and biochemical features and 16S rDNA sequences.Strain BHSN was capable of degrading C13-C32 n-alkanes in crude oil.It grew and produced biosurfactant the most in medium with temperature ranging from 25 to 30 ℃,and pH 5.0~8.5.In LB medium,addition of 10 g·L-1 glucose promoted growth of BHSN,but not hindered production of biosurfactant.Addition of 10 g·L-1 sucrose,lactose,maltose or xylose stimulated both growth and biosurfactant synthesis of BHSN.The biosurfactant produced by Strain BHSN was primarily identified as lipopeptide based on TLC and FT-IR analyses.The biosurfactants it produced in fermented medium decreased the surface tension of the medium from 68.3 to 28.6 mN·m-1.
Key words: petroleum-degrader, Acinetobacter sp., alkane biodegradation, biosurfactant, fermenting condition
CLC Number:
X172
CAO Juan, XU Zhi-Hui, LI Ling-Zhi, SHEN Biao. Biosurfactant-Producing Petroleum-Degrader-Acinetobacter BHSN[J]. Journal of Ecology and Rural Environment, 2009, 25(1): 73-78.
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URL: http://www.ere.ac.cn/EN/CNKI:SUN:NCST.0.2009-01-014
http://www.ere.ac.cn/EN/Y2009/V25/I1/73