青霉素对土壤微生物群落结构的影响

doi:10.11934/j.issn.1673-4831.2016.02.021

生态与农村环境学报 ›› 2016, Vol. 32 ›› Issue (2): 309-314.doi: 10.11934/j.issn.1673-4831.2016.02.021

青霉素对土壤微生物群落结构的影响

刘燕¹, 张振华², 鲍王波², 余冉², 王长永¹

1. 环境保护部南京环境科学研究所, 江苏南京 210042;
2. 东南大学能源与环境学院, 江苏南京 210096

收稿日期:2015-06-26 出版日期:2016-03-25 发布日期:2016-04-01
通讯作者: 余冉,E-mail:yuran@seu.edu.cn;王长永,E-mail:wcy@nies.org E-mail:yuran@seu.edu.cn;wcy@nies.org
作者简介:刘燕(1977-),女,江苏南京人,副研究员,硕士,主要研究方向为生物安全与生物多样性保护。E-mail:liuyan@nies.org
基金资助:
环保公益性行业科研专项(201209024);国家自然科学基金青年基金(41401363);江苏省自然科学基金青年基金(BK20130102);公益性科研院所基本科研业务费项目

Effects of Penicillin on Soil Microbial Community Structure

LIU Yan¹, ZHANG Zhen-hua², BAO Wang-bo², YU Ran², WANG Chang-yong¹

1. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China;
2. Department of Energy and Environment, Southeast University, Nanjing 210096, China

Received:2015-06-26 Online:2016-03-25 Published:2016-04-01

摘要/Abstract

摘要：

利用Biolog-ECO技术分析不同青霉素浓度处理下土壤微生物的群落代谢活性及结构多样性,利用变性梯度凝胶电泳(denaturing gradient gel electrophoresis, DGGE)技术分析典型微生物群落结构变化情况,探讨青霉素对土壤微生物群落的生态毒理效应。主成分分析(PCA)结果表明,高浓度青霉素(400和800 mg·kg^-1)处理能快速诱导土壤中以酚酸类化合物为碳源的微生物菌群富集。DGGE结果显示,在高浓度青霉素(800 mg·kg^-1)胁迫初期,土壤中微生物多样性指数(Shannon-Wiener index)显著降低(P<0.05);7 d后,主要微生物群落结构随土壤中青霉素降解又逐渐恢复。

关键词: 青霉素, 微生物多样性, BiologEcoplates^TM, PCR-DGGE

Abstract:

Effect of penicillin, varying in concentration, on metabolic activity and structural diversity of soil microbial community was analyzed with the Biolog-ECO technology, and changes in the soil microbial community structure were with the DGGE (denaturing gradient gel electrophoresis) technology, so as to explore eco-toxicological effect of penicillin on soil microbial community. PCA based on the BiologEcoplates^TM shows that penicillin high in concentration (400-800 mg·kg^-1) may induce rapid enrichment of microbial groups that use phenolic compounds as carbon source in the soil microbial community. PCR-DGGE analysis reveals that in the soil treated with a high dose(800 mg·kg^-1) of penicillin, Shannon-Wiener diversity index was significantly lowered in the first days and gradual recovered 7 days later with the penicillin in the soil degrading.

Key words: penicillin, microbial diversity, BiologEcoplates^TM, PCR-DGGE

中图分类号:

刘燕, 张振华, 鲍王波, 余冉, 王长永. 青霉素对土壤微生物群落结构的影响[J]. 生态与农村环境学报, 2016, 32(2): 309-314.

LIU Yan, ZHANG Zhen-hua, BAO Wang-bo, YU Ran, WANG Chang-yong. Effects of Penicillin on Soil Microbial Community Structure[J]. Journal of Ecology and Rural Environment, 2016, 32(2): 309-314.

参考文献

[1] 李再兴,田宝阔,左剑恶.抗生素菌渣处理处置技术进展[J].环境工程,2014(30):72-75.
[2] THIELE-BRUHN S,BECK I.Effects of Sulfonamide and Tetracycline Antibiotics on Soil Microbial Activity and Microbial Biomass[J].Chemosphere,2005,59(4):457-465.
[3] ZIELEZNY Y,GROENEWEG J,VEREECKEN H,et al.Impact of Sulfadiazine and Chlorotetracycline on Soil Bacterial Community Structure and Respiratory Activity[J].Soil Biology & Biochemisty,2006,38(8):2372-2380.
[4] BOLEAS S,ALONSO C,PRO J,et al.Toxicity of the Antimicrobial Oxytetracycline to Soil Organisms in a Multi-Species-Soil System (MS Center Dot 3) and Influence of Manure Co-Addition[J].Journal of Hazardous Materials,2005,122(3):233-241.
[5] 刁晓平,孙英健,孙振钧,等.安普霉素对不同土壤中微生物活动的影响[J].生态环境,2004,13(4):565-568.
[6] 章明奎,刘兆云.猪粪和土霉素对不同肥力土壤微生物数量及活性的影响[J].应用生态学报,2009,20(11):2790-2798.
[7] 谢显传,张少华,王冬生,等.阿维菌素对蔬菜地土壤微生物及土壤酶的生态毒理效应[J].土壤学报,2007,44(4):740-743.
[8] MUYZER G,DEWAAL E C,UITTERLINDEN A G.Profiling of Complex Microbial Populations by Denaturing Gradient Gel Electrophoresis Analysis of Polymerase Chain Reaction-Amplified Genes Coding for 16S rRNA[J].Applied Environmental Microbiology,1993,59(3):695-700.
[9] 魏松林.抗生素污染土壤细菌生态学及其耐药性研究[D].扬州:扬州大学,2008.
[10] WESTERGAARD K,MÜLLER A K,CHRISTENSEN S,et al.Effects of Tylosin as a Disturbance on the Soil Microbial Community[J].Soil Biology and Biochemistry,2001,33(15):2061-2071.
[11] 郑华,欧阳志云,方治国,等.BIOLOG在土壤微生物群落功能多样性研究中的应用[J].土壤学报,2004,41(3):456-461.
[12] 刘文娜.兽用抗生素胁迫下农田土壤微生物效应研究[D].北京:中国科学院研究生院,2012.
[13] 唐非凡.金霉素、磺胺嘧啶在土壤中的降解特征及其对土壤微生物的影响[D].杭州:浙江大学,2012.
[14] 张振华,马珊珊,刘燕等.青霉素钠在土壤中的吸附和淋溶特性研究[J].生态与农村环境学报,2014,30(6):749-753.
[15] 贾夏,董岁明,周春娟.微生物生态研究中Biolog Eco微平板培养时间对分析结果的影响[J].应用基础与工程科学学报,2013(1):10-19.
[16] WATANABE T,ASAKAWA S,NAKAMURA A,et al.DGGE Method for Analyzing 16S rDNA of Methanogenic Archaeal Community in Paddy Field Soil[J].FEMS Microbiology Letters,2004,232(2):153-163.
[17] YU Z,MORRISON M.Comparisons of Different Hypervariable Regions of Genes for Use in Fingerprinting of Microbial Communities by PCR-Denaturing Gradient Gel Electrophoresis[J].Applied and Environmental Microbiology,2004,70(8):4800-4806.
[18] PIELOU E C.An Introduction to Mathematical Ecology[M].New York,USA:Wiley Interscience,John Wiley & Sons,1969:105-107.
[19] HIRSCH R,TERNES T,HABERER K,et al.Occurrence of Antibiotics in the Aquatic Environment[J].Science of the Total Environment,1999,225(1/2):109-118.
[20] SACHER F,LANG F T,BRAUCH H J,et al.Pharmaceuticals in Groundwaters-Analytical Methods and Results of a Monitoring Program in Baden-Wurttemberg,Germany[J].Journal of Chromatography A,2001,938(1/2):199-210.
[21] BUSH K,JACOBY G A,MEDEIROS A A.A Functional Classification Scheme for Beta-Lactometersandits Correlation With Molecular-Structure[J].Antimicrobial Agents and Chemotherapy,1995,39(6):1211-1233.
[22] ZHANG Q,DICK W A.Growth of Soil Bacteria,on Penicillin and Neomycin,Not Previously Exposed to These Antibiotics[J].Science of the Total Environment,2014,493:445-453.
[23] COLQUHOUN D R,HARTMANN E M,HALDEN R U.Proteomic Profiling of the Dioxin-Degrading Bacterium Sphungomonas wittichii RW1[J].Journal of Biomedicine and Biotechnology,2012(3):408690.
[24] NITHYA C,PANDIAN S K.Isolation of Heterotrophic Bacteria From Palk Bay Sediments Showing Heavy Metal Tolerance and Antibiotic Production[J].Microbiological Research,2010,165(7):578-593.
[25] JURADO V.Agromycesitalicus sp. nov.,Agromyces humatus sp. nov.and Agromyces lapidis sp. nov. Isolated From Roman Catacombs[J].International Journal of Systematic and Evolutionary Microbiology,2005,55(2):871-875.

青霉素对土壤微生物群落结构的影响

Effects of Penicillin on Soil Microbial Community Structure

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