施氏假单胞菌PFS-4的筛选、优化固定及其对二氯喹啉酸的降解性能

doi:10.11934/j.issn.1673-4831.2016.06.024

生态与农村环境学报 ›› 2016, Vol. 32 ›› Issue (6): 1018-1023.doi: 10.11934/j.issn.1673-4831.2016.06.024

施氏假单胞菌PFS-4的筛选、优化固定及其对二氯喹啉酸的降解性能

张可¹, 吴艺琪², 陈伟¹, 格桑², 陈佳¹, 罗鸿兵¹

1. 四川农业大学土木工程学院, 四川都江堰 611830;
2. 哈尔滨工业大学市政环境工程学院, 黑龙江哈尔滨 150090

出版日期:2016-11-25 发布日期:2016-11-30
通讯作者: 罗鸿兵,E-mail:luohongbing66@163.com E-mail:luohongbing66@163.com
作者简介:张可(1982-),女,四川成都人,讲师,硕士,主要从事环境微生物及市政污水处理研究。E-mail:zhangke@sicau.edu.cn
基金资助:
国家自然科学基金（51278318）；四川省科技支撑计划（2013SZ0103，2014NZ0044）

Isolation, Immobilization and Characterization of Quinclorac-Degrading Strain Pseudomonas stutzeri PFS-4

ZHANG Ke¹, WU Yi-qi², CHEN Wei¹, GE Sang², CHEN Jia¹, LUO Hong-bing¹

1. College of Civil Engineering, Sichuan Agricultural University, Dujiangyan 611830, China;
2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China

Online:2016-11-25 Published:2016-11-30

摘要/Abstract

摘要：

从长期施用二氯喹啉酸的土壤中分离到1株能以二氯喹啉酸为碳源生长的菌株，命名为PFS-4。经16S rRNA基因序列和生理生化特性分析，将菌株PFS-4鉴定为施氏假单胞菌（Pseudomonas stutzeri）。以麦秆吸附-海藻酸钠包埋方式对菌株进行复合固定，采用正交实验对固定条件进行优化，研究温度、pH值、碳源对固定化菌剂降解二氯喹啉酸的影响；考察固定化菌剂对污水中二氯喹啉酸的去除效果，并对比分析游离菌及固定化菌剂去除能力的差异。结果表明，固定化菌剂制备的最佳条件为：海藻酸钠质量分数为5%，CaCl₂为4%，菌胶比1：2，交联时间5 h。在温度为30℃、pH值为7的条件下，经5 d培养，固定化菌剂对500 mg·L^-1二氯喹啉酸降解率为92.3%。在处理污水中二氯喹啉酸时，游离菌的降解能力受到极显著抑制（P<0.01），而对固定化菌剂降解率影响相对较小，去除率保持在64%以上。麦秆吸附-海藻酸钠包埋固定化菌剂对不良环境具有较好缓冲性，可用于微生物降解菌的开发利用。

关键词: 二氯喹啉酸, 麦秆-海藻酸钠复合载体, 固定化, 微生物降解

Abstract:

Using quinclorac as carbon source, one strain, named PFS-4, was isolated from long-term contaminated soil. Strain PFS-4 was identified as Pseudomonas stutzeri based on physiological and biochemical characteristics and 16S rRNA sequence analysis. PFS-4 immobilization condition was optimized by orthogonal experiment, and then the effects of different temperature, initial pH, carbon and nitrogen sources on the degradation of quinclorac by the immobilized bacteria were examined. The performance of free bacteria and immobilized bacteria in actual wastewater were also detected. The results show that the optimal conditions for immobilization were as follows:alginate concentration 5%, calcium chloride 4%, bacteria cement ratio 1:2, crosslinking time 5 h. The immobilized bacteria could effectively degrad 92.3% of quinclorac (500 mg·L^-1) after 5 d inoculation at the condition of 30℃ and pH 7.0. The results also revealed that the degradation rate decreased when treating actual wastewater. The degradation rate of free bacteria was significantly inhibited (P<0.01)when treating wastewater, on the contrary,immobilized bacteria were less affected and the removal rate remained above 64%. Therefore, wheat-straw adsorption-sodium alginate immobilized bacteria can be applied in quinclorac biodegradation treatment because of its good buffering to adverse circumstances.

Key words: quinclorac, wheat straw-SA carrier, immobilization, biodegradation

中图分类号:

X592

张可, 吴艺琪, 陈伟, 格桑, 陈佳, 罗鸿兵. 施氏假单胞菌PFS-4的筛选、优化固定及其对二氯喹啉酸的降解性能[J]. 生态与农村环境学报, 2016, 32(6): 1018-1023.

ZHANG Ke, WU Yi-qi, CHEN Wei, GE Sang, CHEN Jia, LUO Hong-bing. Isolation, Immobilization and Characterization of Quinclorac-Degrading Strain Pseudomonas stutzeri PFS-4[J]. Journal of Ecology and Rural Environment, 2016, 32(6): 1018-1023.

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施氏假单胞菌PFS-4的筛选、优化固定及其对二氯喹啉酸的降解性能

Isolation, Immobilization and Characterization of Quinclorac-Degrading Strain Pseudomonas stutzeri PFS-4

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