4-壬基酚对斑马鱼(Danio rerio)胚胎/仔鱼的毒性效应

doi:10.11934/j.issn.1673-4831.2017.08.009

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (8): 737-742.doi: 10.11934/j.issn.1673-4831.2017.08.009

4-壬基酚对斑马鱼(Danio rerio)胚胎/仔鱼的毒性效应

张慧^1,2, 姜锦林¹, 张宇峰², 单正军¹, 卜元卿¹, 田丰¹

1. 环境保护部南京环境科学研究所/国家环境保护农药环境评价与污染控制重点实验室, 江苏南京 210042;
2. 南京工业大学环境学院, 江苏南京 210009

收稿日期:2016-08-18 出版日期:2017-08-25 发布日期:2017-09-25
通讯作者: 姜锦林,E-mail:jjl@nies.org;张宇峰,E-mail:zhangyuf99@126.com E-mail:jjl@nies.org;zhangyuf99@126.com
作者简介:张慧(1990-),女,江苏淮安人,硕士生,研究方向为环境生态学。E-mail:836742441@qq.com
基金资助:
环保公益性行业科研专项（201309026）

Toxic Effects of 4-Nonyl Phenol on Zebrafish (Danio rerio) Embryo/Larva

ZHANG Hui^1,2, JIANG Jin-lin¹, ZHANG Yu-feng², SHAN Zheng-jun¹, BU Yuan-qing¹, TIAN Feng¹

1. Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China;
2. College of Environment, Nanjing Tech University, Nanjing 210009, China

Received:2016-08-18 Online:2017-08-25 Published:2017-09-25

摘要/Abstract

摘要：

4-壬基酚（4-NP）作为一种稳定的环境内分泌干扰物，会对生物体的生长发育造成影响。以斑马鱼（Danio rerio）胚胎为供试体，研究4-壬基酚对斑马鱼胚胎生长发育的毒性效应。斑马鱼胚胎被暴露在一系列浓度的4-壬基酚溶液中（2.0、5.0、10.0、25.0、50.0和125.0 μg·L^-1），暴露120 h后空白组初孵仔鱼因饥饿出现死亡即停止实验。记录暴露120 h内斑马鱼胚胎的死亡数、孵化数，畸形数、仔鱼体长及30 s心跳数。结果表明，4-壬基酚对斑马鱼胚胎48、72和96 h的半致死浓度（LC₅₀）分别为131.4、47.2和26.8 μg·L^-1；125.0 μg·L^-1的4-壬基酚对斑马鱼胚胎的高致死效应使其孵化率显著降低；与空白对照组相比，4-壬基酚的暴露可显著提高斑马鱼胚胎尾部畸形率和仔鱼的心包囊肿率，其中斑马鱼胚胎尾部畸形率随处理浓度的增加而增加，呈正剂量-效应关系；经4-壬基酚暴露处理的斑马鱼仔鱼的体长及30 s心跳数较空白对照组均显著下降。2.0 μg·L^-1 4-壬基酚对斑马鱼胚胎及仔鱼即具有致畸和致死效应，对其胚胎的发育有不利影响。

关键词: 4-壬基酚, 斑马鱼, 胚胎发育, 毒性效应

Abstract:

4-Nonylphenol (4-NP), a stable environmental endocrine disruptor, exerts negative effects on the growth and development of organisms. Danio rerio embryos were exposed to a series of concentrations of 4-NP at 2.0, 5.0, 10.0, 25.0, 50.0 and 125.0 μg·L^-1 for 120 h. During the exposure, the mortality, hatching rates and abnormalities of embryos, as well as the body length and heart rates in 30 s were recorded everyday till the larva in the control group began to die as a result of hunger. The results show that the LC₅₀ of Danio rerio embryos exposed to 4-NP after 48, 72 and 96 h were 131.4, 47.2 and 26.8 μg·L^-1, respectively. The hatching rate of Danio rerio embryos significantly declined due to the lethal effect of 125.0 μg·L^-1 of 4-NP. In addition, exposure to 4-NP significantly increased the tail malformation and pericardial cyst rates of Danio rerio embryos. A positive dose-response relationship was observed in the tail malformation rates. Compared to the control group, the Danio rerio larva in all 4-NP treatment groups had shorter body length and less 30 s heart rates. These results suggested that exposure to 2.0 μg·L^-1 concentration of 4-NP can exert teratogenic and lethal effects on the Danio rerio embryos.

Key words: 4-nonylphenol, Danio rerio, embryonic development, toxic effect

中图分类号:

X592

张慧, 姜锦林, 张宇峰, 单正军, 卜元卿, 田丰. 4-壬基酚对斑马鱼(Danio rerio)胚胎/仔鱼的毒性效应[J]. 生态与农村环境学报, 2017, 33(8): 737-742.

ZHANG Hui, JIANG Jin-lin, ZHANG Yu-feng, SHAN Zheng-jun, BU Yuan-qing, TIAN Feng. Toxic Effects of 4-Nonyl Phenol on Zebrafish (Danio rerio) Embryo/Larva[J]. Journal of Ecology and Rural Environment, 2017, 33(8): 737-742.

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4-壬基酚对斑马鱼(Danio rerio)胚胎/仔鱼的毒性效应

Toxic Effects of 4-Nonyl Phenol on Zebrafish (Danio rerio) Embryo/Larva

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