磺胺-磺胺二甲基嘧啶污染对小白菜生长和生理指标的影响及相关性分析

doi:10.11934/j.issn.1673-4831.2018.06.011

摘要/Abstract

摘要：

采用种子萌发和水培实验研究磺胺（SA）和磺胺二甲基嘧啶（SMT）单一及复合污染对小白菜（Brassica campestris）生长及生理的影响，并对SA、SMT和小白菜生长生理指标进行相关分析。结果表明，低浓度磺胺类抗生素（SAs）污染对小白菜种子萌发的影响较小；单一及复合污染条件下小白菜的生长及品质均受到影响，随着SAs污染浓度的增加，小白菜的根伸长、芽伸长、鲜重和干重抑制作用均增加，小白菜中叶绿素、可溶性蛋白质、过氧化物酶（POD）活性及氧化氢酶（CAT）活性也呈总体下降趋势；单一污染下丙二醛（MDA）含量随SA浓度增加而增加，而MDA含量随SMT浓度增加呈先增加后降低趋势，复合污染下MDA含量呈下降趋势。Pearson相关性分析发现，SAs单一及复合污染条件下SAs分别与小白菜的可溶性蛋白质、叶绿素、CAT活性和POD活性的相关性较好。在SAs污染下，小白菜的生理生长指标及品质均受不同程度的影响，小白菜体内SAs含量随着SAs污染浓度增加而增加，且对SMT的富集大于SA。SAs可以通过富集进入小白菜体内，存在潜在的食品安全风险。

关键词: 小白菜, 磺胺, 生长生理, 相关性分析

Abstract:

The effects of single and compound pollution of sulfonamides (SA) and sulfamethazine (SMT) on the growth and physiology of Brassica campestris were studied by seed germination and hydroponic experiments. The correlations between Brassica campestris growth & physiological indexes with SA, SMT were analyzed. The results showed that the effect of low concentration of SAs on the germination of Brassica campestris seeds was not significant. Under single and combined pollution conditions, compared with the control group, the growth and quality of Brassica campestris were affected. The inhibitory effects on root elongation, shoot elongation, fresh weight and dry weight of Brassica campestris increased with the increase of SAs concentration, the content of chlorophyll and soluble protein in Brassica campestris showed a general downward trend. Under single pollution, the content of malondialdehyde (MDA) increased with the increase of SA concentration. With the increase of SMT concentration, the content of MDA increased at first and then decreased, while the MDA content decreased with compound pollution. The content of SAs in Brassica campestris increased with the increase of SAs pollution concentration, and the enrichment of SMT was greater than that of SA. Pearson correlation analysis showed that SAs had a good correlation with soluble protein, chlorophyll, CAT activity and POD activity of Brassica campestris under single and combined pollution conditions,respectively. Under the SAs pollution, the physiological growth indexes and quality of Brassica campestris were affected by different degrees. The SAs content in Brassica campestris increased with the increase of SAs pollution concentration, and the enrichment of SMT was greater than that of SA. SAs can be enriched into Brassica campestris leading to potential food safety risks.

Key words: Brassica campestris, sulfonamides, growth &, physiology, correlation analysis

中图分类号:

X592

司雄元, 熊科胜, 徐慧敏, 檀华蓉, 司友斌. 磺胺-磺胺二甲基嘧啶污染对小白菜生长和生理指标的影响及相关性分析[J]. 生态与农村环境学报, 2018, 34(6): 554-562.

SI Xiong-yuan, XIONG Ke-sheng, XU Hui-min, TAN Hua-rong, SI You-bin. Effects of Sulfonamide-Sulfamethazine on Growth and Physiological Indexes of Brassica campestris and Its Correlation Analysis[J]. Journal of Ecology and Rural Environment, 2018, 34(6): 554-562.

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