基于MicroRespTM不同施肥方式对土壤微生物群落的影响

doi:10.11934/j.issn.1673-4831.2018.07.007

摘要/Abstract

摘要：

土壤微生物群落代谢变化被认为是反映土壤质量和健康状况的敏感指标。通过5 a定位试验，采用MicroResp^TM方法分析不同施肥方式对农田土壤微生物群落功能多样性及其代谢结构的影响。结果表明，适量施用有机肥和沼液肥有利于提高土壤微生物代谢活性，并对土壤微生物群落代谢结构产生明显影响，但长期高量施用沼液肥不利于维持土壤微生物代谢活性和群落多样性。相关性分析结果表明，土壤pH值、全磷含量和Cu含量与土壤微生物代谢活性及其群落结构关系最密切，其中土壤Cu含量为主要影响因子。能明显提高土壤养分含量及导致土壤重金属Cu、Zn累积是有机肥较其他施肥方式对土壤微生物群落代谢功能产生显著影响的关键因素。

关键词: 施肥, 微生物群落功能多样性, 微生物群落代谢结构, MicroResp^TM

Abstract:

The response of soil microbial community is regarded as a sensitive indicator of soil quality changes. A 5-year located experiment was conducted to study the effects of different fertilization regimes on the soil microbial community structure and functional diversity, by using the MicroResp^TM method. The results show that the application of organic fertilizer and biogas slurry at a reasonable rate could significantly promote the metabolic activity of soil microbial community and significantly affect the soil microbial community structure. Long-term application of organic fertilizer and biogas slurry at a high amount such as biogas slurry (HBS) treatment and organic fertilizer (HOM) treatment exhibited negative effects on the soil microbial community structure and functional diversity. Correlation analysis results further indicate that the metabolic activity of soil microbial community was highly correlated with the soil pH, the total phosphorus content and the Cu content, while the Cu content was the main influencing factor. Overall, organic fertilizer application could significantly improve soil nutrient content and lead to an accumulation of heavy metals in soil, thus contributing to the obvious changes in metabolic function of soil microbial community.

Key words: fertilization regimes, microbial community functional diversity, microbial community metabolic structure, MicroResp^TM

中图分类号:

S154.3

王飞, 秦方锦, 王先挺, 翁颖, 林辉. 基于MicroResp^TM不同施肥方式对土壤微生物群落的影响[J]. 生态与农村环境学报, 2018, 34(7): 622-629.

WANG Fei, QIN Fang-jin, WANG Xian-ting, WENG Ying, LIN Hui. Study on Effects of Different Fertilization Regimes on Soil Microbial Community Based on MicroResp^TM Method[J]. Journal of Ecology and Rural Environment, 2018, 34(7): 622-629.

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