云南不同高原富营养化湖泊噬藻体g20基因遗传多样性研究

doi:10.19741/j.issn.1673-4831.2020.0978

生态与农村环境学报 ›› 2021, Vol. 37 ›› Issue (6): 786-793.doi: 10.19741/j.issn.1673-4831.2020.0978

云南不同高原富营养化湖泊噬藻体g20基因遗传多样性研究

蔡一鸣, 刘玉珊, 王志龙, 李春筱, 刘丽

昆明理工大学生命科学与技术学院, 云南昆明 650500

收稿日期:2020-12-05 出版日期:2021-06-25 发布日期:2021-06-24
通讯作者: 刘丽 E-mail:liuli2272@163.com
作者简介:蔡一鸣(1995-),男,山东滨州人,主要从事环境生物学方面的研究。E-mail:547288269@qq.com
基金资助:
国家自然科学基金（31860028，31360016）

Phylogenetic Diversity of Cyanophage g20 Gene in Different Eutrophic Plateau Lakes in Yunnan Province

CAI Yi-ming, LIU Yu-shan, WANG Zhi-long, LI Chun-xiao, LIU Li

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China

Received:2020-12-05 Online:2021-06-25 Published:2021-06-24

摘要/Abstract

摘要： 为探索云南不同高原湖泊噬藻体g20基因的多样性，以期更深入地了解噬藻体遗传多样性以及发现噬藻体新的基因型，以云南高原不同富营养化淡水湖泊（滇池、星云湖和抚仙湖）为研究对象，采用PCR-克隆技术，以g20基因为标记基因，对2018年秋冬季3个湖泊水体中噬藻体多样性进行研究，构建系统进化树，分析3个湖泊中噬藻体g20基因的遗传多样性。从3个湖泊水样中获得43条噬藻体g20基因环境序列，系统进化分析表明，云南高原湖泊噬藻体g20基因分布与湖泊、海洋水体中噬藻体g20基因亲缘关系较近，但远离稻田水体g20基因的分布；各湖泊秋冬季g20基因遗传多样性无明显差异。云南高原湖泊水体中噬藻体g20基因遗传多样性较为丰富，存在新的噬藻体类群。

关键词: 噬藻体, 遗传多样性, g20基因, 高原湖泊

Abstract: In order to investigate the genetic diversity and distribution of g20 gene of cyanophages among different eutrophic plateau lakes in Yunnan Province, water samples were collected from Dianchi Lake, Xingyun Lake and Fuxian Lake. The phylogenetic tree was constructed to analyze the phylogenetic diversity of cyanophage g20 genes in the autumn and winter of 2018. A total of 43 g20 genes of cyanophage were cloned and sequenced. Phylogenetic analysis show that the distribution of cyanophage g20 genes in water samples of these lakes was closely related to those in other lakes and oceans, but far from those in paddy water. Between autumn and winter, the genetic diversity of g20 genes among the lakes was not significantly different. The genetic diversity of the cyanophage g20 genes of the three plateau lakes in Yunnan province is relatively rich, and novel groups of g20 genes are existing.

Key words: cyanophage, genetic diversity, g20 gene, plateau lake

中图分类号:

X52
[Q89]

蔡一鸣, 刘玉珊, 王志龙, 李春筱, 刘丽. 云南不同高原富营养化湖泊噬藻体g20基因遗传多样性研究[J]. 生态与农村环境学报, 2021, 37(6): 786-793.

CAI Yi-ming, LIU Yu-shan, WANG Zhi-long, LI Chun-xiao, LIU Li. Phylogenetic Diversity of Cyanophage g20 Gene in Different Eutrophic Plateau Lakes in Yunnan Province[J]. Journal of Ecology and Rural Environment, 2021, 37(6): 786-793.

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云南不同高原富营养化湖泊噬藻体g20基因遗传多样性研究

Phylogenetic Diversity of Cyanophage g20 Gene in Different Eutrophic Plateau Lakes in Yunnan Province

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