有机配体对超富集植物转运及解毒镍、锌的影响

doi:10.19741/j.issn.1673-4831.2022.0374

生态与农村环境学报 ›› 2023, Vol. 39 ›› Issue (10): 1239-1246.doi: 10.19741/j.issn.1673-4831.2022.0374

有机配体对超富集植物转运及解毒镍、锌的影响

莫冰岚¹, 李蕾², 胡尊河¹, 邓腾灏博², 曹越³, 汤叶涛³, 仇荣亮^1,3,4,5

1. 华南农业大学资源环境学院, 广东广州 510642;
2. 广东省农业科学院农业质量标准与监测技术研究所, 广东广州 510640;
3. 中山大学环境科学与工程学院, 广东广州 510006;
4. 岭南现代农业科学与技术广东省实验室, 广东广州 510642;
5. 广东省农业农村污染治理与环境安全重点实验室, 广东广州 510642

收稿日期:2022-04-25 出版日期:2023-10-25 发布日期:2023-10-23
通讯作者: 邓腾灏博,E-mail:dengtenghb@gdaas.cn E-mail:dengtenghb@gdaas.cn
作者简介:莫冰岚(1998-),女,广西贵港人,主要从事污染环境修复技术研究。E-mail:mobinglan@stu.scau.edu.cn
基金资助:
国家自然科学基金重点国际(地区)合作研究项目(41920104003)

The Effect of Organic Ligands on the Transport and Detoxification Processes of Nickel and Zinc in Hyperaccumulators

MO Bing-lan¹, LI Lei², HU Zun-he¹, DENG Teng-hao-bo², CAO Yue³, TANG Ye-tao³, QIU Rong-liang^1,3,4,5

1. College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China;
2. Institute of Quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of Sciences, Guangzhou 510640, China;
3. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China;
4. Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China;
5. Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, Guangzhou 510642, China

Received:2022-04-25 Online:2023-10-25 Published:2023-10-23

摘要/Abstract

摘要： 超富集植物是一类能大量吸收重金属并将其转运至地上部的特殊植物。这类植物体内通常含有高量的有机配体,如烟草胺、组氨酸等,可与其形成稳定的金属-配体复合物。现阶段针对超富集植物体内Ni、Zn的有机配体的研究较为充分。该研究综述了在根部运输及解毒、木质部转运和叶片解毒过程中有机配体与重金属的相互作用关系、超富集植物合成有机配体的分子机制以及有机配体和金属配合物的检测方法。综合来看,氨基酸类配体可能促进Ni、Zn在根部共质体中的迁移并缓解过量Ni、Zn的细胞毒性;在木质部运输过程中Ni、Zn则主要以水合离子形式赋存,与配体结合的比例相对较低;叶部赋存的Ni、Zn则主要通过与有机酸结合的形式进行解毒。由于现有的研究大部分针对有限的几种超富集植物及有限的几种有机配体,未来还需结合代谢组学及转录组学探索更多植物品种有机配体特征及其解毒的分子机制,从而为厘清植物超富集重金属的相关机制及其在植物提取方面的应用提供理论依据。

关键词: 配体, 锌, 镍, 超富集植物

Abstract: Hyperaccumulators are a group of specialized plants that absorb large amounts of heavy metals and store most of them in their aboveground parts. These plants are rich in organic ligands, such as nicotianamine and histidine, which can combine with metals to form metal-ligand complexes. At present, the studies on nickel and zinc organic ligands in plants are relatively sufficient. Therefore, this paper focuses on the interaction between organic ligands and heavy metals during root transport and detoxification, xylem transport and leaf detoxification processes, the molecular mechanism of organic ligand synthesis in hyperaccumulators, as well as methods for detection of organic ligands and metal complexes. To sum up, organic ligands may promote the migration of Ni and Zn in the symplasts of hyperaccumulators and alleviate the cytotoxicity of excess Ni and Zn. In the process of xylem transport, nickel and zinc mainly occur by hydrated ions, but the role of organic ligands is relatively weak in this process. Nickel and zinc mainly combine with organic acid for leaf detoxification. Since most of the findings come from a limited number of hyperaccumulators and organic ligands, it is necessary to combine metabonomics and transcriptomics to study the characteristics and molecular mechanisms about detoxification of organic ligands in more species of hyperaccumulators in the future, so as to provide theoretical basis for clarifying the relevant mechanisms of selective hyperaccumulation of heavy metals in plants and for the application of hyperaccumulators in phytoextraction.

Key words: ligands, zinc, nickel, hyperaccumulator

中图分类号:

莫冰岚, 李蕾, 胡尊河, 邓腾灏博, 曹越, 汤叶涛, 仇荣亮. 有机配体对超富集植物转运及解毒镍、锌的影响[J]. 生态与农村环境学报, 2023, 39(10): 1239-1246.

MO Bing-lan, LI Lei, HU Zun-he, DENG Teng-hao-bo, CAO Yue, TANG Ye-tao, QIU Rong-liang. The Effect of Organic Ligands on the Transport and Detoxification Processes of Nickel and Zinc in Hyperaccumulators[J]. Journal of Ecology and Rural Environment, 2023, 39(10): 1239-1246.

参考文献 67

[1]	BROOKS R R,LEE J,REEVES R D,et al.Detection of Nickeliferous Rocks by Analysis of Herbarium Specimens of Indicator Plants[J].Journal of Geochemical Exploration,1977,7:49-57.
[2]	BROOKS R R,MORRISON R S,REEVES R D,et al.Hyperaccumulation of Nickel by Alyssum linnaeus (Cruciferae)[J].Proceedings of the Royal Society of London:Series B,Biological Sciences,1979,203(1153):387-403.
[3]	BAKER A J M.Metal Tolerance[J].New Phytologist,2008,106:93-111.
[4]	CHANEY R.Plant Uptake of Inorganic Waste Constituents[J]. Land Treatment of Hazardous Wastes,1983,5:50-76.
[5]	VAN DER ENT A,BAKER A J M,REEVES R D,et al.Hyperaccumulators of Metal and Metalloid Trace Elements:Facts and Fiction[J].Plant and Soil,2013,362(1):319-334.
[6]	REEVES R D,VAN DER ENT A,BAKER A J M.Global Distribution and Ecology of Hyperaccumulator Plants[M]//VAN DER ENT A,ECHEVARRIA G,BAKER A,et al.Agromining:Farming for Metals.Cham,USA:Springer,2018:75-92.
[7]	The University of Queensland,Australia.Global Hyperaccumulator Database[EB/OL].[2022-04-12].http://hyperaccumulators.smi.uq.edu.au/collection/.
[8]	何玉君,孙梦荷,沈亚婷,等.超富集植物与重金属相互作用机制及应用研究进展[J].岩矿测试,2020,39(5):639-657.[HE Yu-jun,SUN Meng-he,SHEN Ya-ting,et al.Research Progress on the Interaction Mechanism between Hyperaccumulator and Heavy Metals and Its Application[J].Rock and Mineral Analysis,2020,39(5):639-657.]
[9]	KRÄMER U.Metal Hyperaccumulation in Plants[J].Annual Review of Plant Biology,2010,61:517-534.
[10]	孙琴,倪吾钟,杨肖娥.超积累植物体内的小分子螯合物质及其生理作用[J].广东微量元素科学,2001,8(5):1-8.[SUN Qin,NI Wu-zhong,YANG Xiao-e.Low Molecular Weight Chelating Compounds and Their Physiological Function in Hyperaccumulator[J].Trace Elements Science,2001,8(5):1-8.]
[11]	INGLE R A.Histidine Biosynthesis[J].The Arabidopsis Book,2011,9:e0141.
[12]	SALT D E,KATO N,KRÄMER U,et al.The Role of Root Exudates in Nickel Hyperaccumulation and Tolerance in Accumulator and Nonaccumulator Species of Thlaspi[M]// TERRY N,BAÑUELOS G S.Phytoremediation of Contaminated Soil and Water.Boca Raton,USA:CRC Press,2020:189-200.
[13]	SALT D E,PRINCE R C,BAKER A J M,et al.Zinc Ligands in the Metal Hyperaccumulator Thlaspi caerulescens as Determined Using X-Ray Absorption Spectroscopy[J].Environmental Science & Technology,1999,33(5):713-717.
[14]	SEREGIN I V,KOZHEVNIKOVA A D.Low-molecular-weight Ligands in Plants:Role in Metal Homeostasis and Hyperaccumulation[J].Photosynthesis Research,2020,150(1/2/3):51-96.
[15]	SCHOLZ G,BECKER R,PICH A,et al.Nicotianamine-a Common Constituent of Strategies Ⅰ and Ⅱ of Iron Acquisition by Plants:A Review[J].Journal of Plant Nutrition,1992,15(10):1647-1665.
[16]	PICH A,SCHOLZ G,STEPHAN U W.Iron-Dependent Changes of Heavy Metals,Nicotianamine,and Citrate in Different Plant Organs and in the Xylem Exudate of Two Tomato Genotypes.Nicotianamine as Possible Copper Translocator[J].Plant and Soil,1994,165(2):189-196.
[17]	TRAMPCZYNSKA A,KÜPPER H,MEYER-KLAUCKE W,et al.Nicotianamine Forms Complexes with Zn(Ⅱ)in Vivo[J].Metallomics,2010,2(1):57-66.
[18]	BLINDAUER C A,SCHMID R.Cytosolic Metal Handling in Plants:Determinants for Zinc Specificity in Metal Transporters and Metallothioneins[J].Metallomics,2010,2(8):510-529.
[19]	龙新宪,杨肖娥,叶正钱.超积累植物的金属配位体及其在植物修复中的应用[J].植物生理学通讯,2003,39(1):71-77.[LONG Xin-xian,YANG Xiao-e,YE Zheng-qian.Metal Chelators in Hyperaccumulator and Their Application in Phytoremedi-Ation[J].Plant Physiology Communications,2003,39(1):71-77.]
[20]	朱艳霞,魏幼璋,叶正钱,等.有机酸在超积累植物重金属解毒机制中的作用[J].西北农林科技大学学报(自然科学版),2006,34(7):121-126.[ZHU Yan-xia,WEI You-zhang,YE Zheng-qian,et al.Function of Organic Acids in Heavy Metal Tolerance Mechanism in Hyperaccumulator[J].Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition),2006,34(7):121-126.]
[21]	CLEMENS S,DEINLEIN U,AHMADI H,et al.Nicotianamine Is a Major Player in Plant Zn Homeostasis[J].BioMetals,2013,26(4):623-632.
[22]	HAYDON M J,COBBETT C S.Transporters of Ligands for Essential Metal Ions in Plants[J].New Phytologist,2007,174(3):499-506.
[23]	HARRIS W R,SAMMONS R D,GRABIAK R C.A Speciation Model of Essential Trace Metal Ions in Phloem[J].Journal of Inorganic Biochemistry,2012,116:140-150.
[24]	KERKEB L,KRÄMER U.The Role of Free Histidine in Xylem Loading of Nickel in Alyssum lesbiacum and Brassica juncea[J].Plant Physiology,2003,131(2):716-724.
[25]	RICHAU K H,KOZHEVNIKOVA A D,SEREGIN I V,et al.Chelation by Histidine Inhibits the Vacuolar Sequestration of Nickel in Roots of the Hyperaccumulator Thlaspi caerulescens[J].New Phytologist,2009,183(1):106-116.
[26]	KOZHEVNIKOVA A D,SEREGIN I V,VERWEI J R,et al.Histidine Promotes the Loading of Nickel and Zinc,but not of Cadmium,into the Xylem in Noccaea caerulescens[J].Plant Signaling & Behavior,2014,9(9):e29580.
[27]	KOZHEVNIKOVA A D,SEREGIN I V,ERLIKH N T,et al.Histidine-Mediated Xylem Loading of Zinc is a Species-Wide Character in Noccaea caerulescens[J].The New Phytologist,2014,203(2):508-519.
[28]	CLEMENS S.Metal Ligands in Micronutrient Acquisition and Homeostasis[J].Plant,Cell & Environment,2019,42(10):2902-2912.
[29]	DEINLEIN U,WEBER M,SCHMID T H,et al.Elevated Nicotianamine Levels in Arabidopsis halleri Roots Play a Key Role in Zinc Hyperaccumulation[J].The Plant Cell,2012,24(2):708-723.
[30]	CHEN S N,ZHANG M,FENG Y,et al.Nicotianamine Synthase Gene 1 from the Hyperaccumulator Sedum alfredii Hance Is Associated with Cd/Zn Tolerance and Accumulation in Plants[J].Plant and Soil,2019,443(1/2):413-427.
[31]	PIANELLI K,MARI S,MARQUōS L,et al.Nicotianamine Over-Accumulation Confers Resistance to Nickel in Arabidopsis thaliana[J].Transgenic Research,2005,14(5):739-748.
[32]	MONTARGōS-PELLETIER E,CHARDOT V,ECHEVARRIA G,et al.Identification of Nickel Chelators in Three Hyperaccumulating Plants:An X-Ray Spectroscopic Study[J].Phytochemistry,2008,69(8):1695-1709.
[33]	BOOMINATHAN R,DORAN P M.Organic Acid Complexation,Heavy Metal Distribution and the Effect of ATPase Inhibition in Hairy Roots of Hyperaccumulator Plant Species[J].Journal of Biotechnology,2003,101(2):131-146.
[34]	SARRET G,SAUMITOU-LAPRADE P,BERT V,et al.Forms of Zinc Accumulated in the Hyperaccumulator Arabidopsis halleri[J].Plant Physiology,2002,130(4):1815-1826.
[35]	SHEN Z G,ZHAO F J,MCGRATH S P.Uptake and Transport of Zinc in the Hyperaccumulator Thlaspi caerulescens and the Non-Hyperaccumulator Thlaspi ochroleucum[J].Plant,Cell and Environment,1997,20(7):898-906.
[36]	HANIKENNE M,TALKE I N,HAYDON M J,et al.Evolution of Metal Hyperaccumulation Required Cis-Regulatory Changes and Triplication of HMA4[J].Nature,2008,453(7193):391-395.
[37]	GENDRE D,CZERNIC P,CONÉJÉRO G,et al.TcYSL3,a Member of the YSL Gene Family from the Hyper-accumulator Thlaspi caerulescens,Encodes a Nicotianamine-Ni/Fe Transporter[J].The Plant Journal,2006,49(1):1-15.
[38]	王晓娟,王文斌,杨龙,等.重金属镉(Cd)在植物体内的转运途径及其调控机制[J].生态学报,2015,35(23):7921-7929.[WANG Xiao-juan,WANG Wen-bin,YANG Long,et al.Transport Pathways of Cadmium(Cd) and Its Regulatory Mechanisms in Plant[J].Acta Ecologica Sinica,2015,35(23):7921-7929.]
[39]	林庆宇,李建平,闫研.超积累植物富集机制研究方法进展[J].分析化学,2008,36(3):405-412.[LIN Qing-yu,LI Jian-ping,YAN Yan.Progress on the Methodological Research for Enrichment Mechanism of Hyperaccumulators on Metal Ions[J].Chinese Journal of Analytical Chemistry,2008,36(3):405-412.]
[40]	ALVES S,NABAIS C,DE LURDES S G M,et al.Nickel Speciation in the Xylem Sap of the Hyperaccumulator Alyssum serpyllifolium ssp. lusitanicum Growing on Serpentine Soils of Northeast Portugal[J].Journal of Plant Physiology,2011,168(15):1715-1722.
[41]	CENTOFANTI T,SAYERS Z,CABELLO-CONEJO M I,et al.Xylem Exudate Composition and Root-to-Shoot Nickel Translocation in Alyssum Species[J].Plant and Soil,2013,373(1):59-75.
[42]	LU L L,TIAN S,ZHANG J,et al.Efficient Xylem Transport and Phloem Remobilization of Zn in the Hyperaccumulator Plant Species Sedum alfredii[J].New Phytologist,2013,198(3):721-731.
[43]	DENG T H B,VAN DER ENT A,TANG Y T,et al.Nickel Hyperaccumulation Mechanisms:A Review on the Current State of Knowledge[J].Plant and Soil,2018,423(1):1-11.
[44]	BROADHURST C L,CHANEY R L,ANGLE J S,et al.Nickel Localization and Response to Increasing Ni Soil Levels in Leaves of the Ni Hyperaccumulator Alyssum murale[J].Plant and Soil,2004,265(1):225-242.
[45]	LEE J L,REEVES R D,BROOKS R R,et al.Isolation and Identification of a Citrato-complex of Nickel from Nickel-accumulating Plants[J].Phytochemistry,1977,16(10):1503-1505.
[46]	BROOKS R R,SHAW S,ASENSIMARFIL A.The Chemical Form and Physiological Function of Nickel in Some Iberian Alyssum Species[J].Physiologia Plantarum,1981,51(2):167-170.
[47]	VÁZQUEZ M D,POSCHENRIEDER C,BARCELÓ J,et al.Compartmentation of Zinc in Roots and Leaves of the Zinc Hyperaccumulator Thlaspi caerulescens J & C Presl[J].Botanica Acta,1994,107(4):243-250.
[48]	YANG X E,LI T Q,YANG J C,et al.Zinc Compartmentation in Root,Transport into Xylem,and Absorption into Leaf Cells in the Hyperaccumulating Species of Sedum alfredii Hance[J].Planta,2006,224(1):185-195.
[49]	KÜPPER H,LOMBI E,ZHAO F J,et al.Cellular Compartmentation of Cadmium and Zinc in Relation to other Elements in the Hyperaccumulator Arabidopsis halleri[J].Planta,2000,212(1):75-84.
[50]	TOLRA R,POSCHENRIEDER C,BARCELO J.Zinc Hyperaccumulation in Thlaspi caerulescens:Ⅱ.Influence on Organic Acids[J].Journal of Plant Nutrition,1996,19(12):1541-1550.
[51]	LU L L,LIAO X C,LABAVITCH J,et al.Speciation and Localization of Zn in the Hyperaccumulator Sedum alfredii by Extended X-Ray Absorption Fine Structure and Micro-X-Ray Fluorescence[J].Plant Physiology and Biochemistry,2014,84:224-232.
[52]	TALKE I N,HANIKENNE M,KRÄMER U.Zinc-Dependent Global Transcriptional Control,Transcriptional Deregulation,and Higher Gene Copy Number for Genes in Metal Homeostasis of the Hyperaccumulator Arabidopsis halleri[J].Plant Physiology,2006,142(1):148-167.
[53]	MO X R,ZHU Q Y,LI X,et al.The Hpa1 Mutant of Arabidopsis Reveals a Crucial Role of Histidine homeostasis in Root Meristem Maintenance[J].Plant Physiology,2006,141(4):1425-1435.
[54]	MARI S,GENDRE D,PIANELLI K,et al.Root-to-shoot Long-distance Circulation of Nicotianamine and Nicotianamine-Nickel Chelates in the Metal Hyperaccumulator Thlaspi caerulescens[J].Journal of Experimental Botany,2006,57(15):4111-4122.
[55]	CHEN S N,ALI SAHITO Z,ZHANG M,et al.Identification and Characterization of Four Nicotianamine Synthase Genes in Sedum alfredii Hance[J].Journal of Biobased Materials and Bioenergy,2018,12(6):551-559.
[56]	INGLE R A,MUGFORD S T,REES J D,et al.Constitutively High Expression of the Histidine Biosynthetic Pathway Contributes to Nickel Tolerance in Hyperaccumulator Plants[J].The Plant Cell,2005,17(7):2089-2106.
[57]	PERSANS M W,YAN X,PATNOE J M,et al.Molecular Dissection of the Role of Histidine in Nickel Hyperaccumulation in Thlaspi goesingense (Hálácsy)[J].Plant Physiology,1999,121(4):1117-1126.
[58]	ZHAO L,SUN Y L,CUI S X,et al.Cd-Induced Changes in Leaf Proteome of the Hyperaccumulator Plant Phytolacca americana[J].Chemosphere,2011,85(1):56-66.
[59]	SCHNEIDER T,PERSSON D P,HUSTEDS,et al.A Proteomics Approach to Investigate the Process of Zn Hyperaccumulation in Noccaea caerulescens(J & C.Presl) F.K.Meyer[J].The Plant Journal,2013,73(1):131-142.
[60]	WANG J C,CHEN X F,CHU S H,et al.Comparative Cytology Combined with Transcriptomic and Metabolomic Analyses of Solanum nigrum L.in Response to Cd Toxicity[J].Journal of Hazardous Materials,2022,423:127168.
[61]	VAN DER ENT A,CALLAHAN D L,NOLLER B N,et al.Nickel Biopathways in Tropical Nickel Hyperaccumulating Trees from Sabah (Malaysia)[J].Scientific Reports,2017,7:41861.
[62]	TSEDNEE M,MAK Y W,CHEN Y R,et al.A Sensitive LC-ESI-Q-TOF-MS Method Reveals Novel Phytosiderophores and Phytosiderophore-Iron Complexes in Barley[J].New Phytologist,2012,195(4):951-961.
[63]	彭红云,杨肖娥.金属有机配体分析方法及金属组学研究[J].分析化学,2006,34(8):1190-1196.[PENG Hong-yun,YANG Xiao-e.Analytical Techniques for Metal-organic Ligand and Metallomics Study[J].Chinese Journal of Analytical Chemistry,2006,34(8):1190-1196.]
[64]	MCNEAR D H,CHANEY R L,SPARKS D L.The Hyperaccumulator Alyssum murale Uses Complexation with Nitrogen and Oxygen Donor Ligands for Ni Transport and Storage[J].Phytochemistry,2010,71(2/3):188-200.
[65]	MERDY P,GUILLON E,DUMONCEAU J,et al.Spectroscopic Study of Copper(Ⅱ)-Wheat Straw Cell Wall Residue Surface Complexes[J].Environmental Science & Technology,2002,36(8):1728-1733.
[66]	STRACZEK A,SARRET G,MANCEAU A,et al.Zinc Distribution and Speciation in Roots of Various Genotypes of Tobacco Exposed to Zn[J].Environmental and Experimental Botany,2008,63(1/2/3):80-90.
[67]	UENO D,MA J F,IWASHITA T,et al.Identification of the Form of Cd in the Leaves of a Superior Cd-Accumulating Ecotype of Thlaspi caerulescens Using 113Cd-NMR[J].Planta,2005,221(6):928-936.

有机配体对超富集植物转运及解毒镍、锌的影响

The Effect of Organic Ligands on the Transport and Detoxification Processes of Nickel and Zinc in Hyperaccumulators

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