Abstract:

To explore for optimal fertilization methods for wine bamboo groves in the semi-arid region of Southwest China, bamboo bleeding sap was collected from wounds of wine bamboo poles in groves applied with bamboo biochar-based bio-manure (ZT) or organic manure for analysis of annual collection rate (C_Sap) and nutrition composition of the bleeding sap as basic data for preparing a functional beverage out of the sap and for designing ZT application methods in the wine bamboo groves. Results show that in the groves applied with ZT for the whole year, bleeding sap collection could last from May (beginning of the rainy season) till November (beginning of the dry season), with the prime season covering the four months between July and October and the sap yielding peak(2.66 L)occurring in September, and traces of sap flow could still be observed in January, which indicates that the effect of ZT on sap yielding was significant. Besides, the data on soil water content and soil temperature in the soil layer 10-15 cm in depth in the dry season also indicate that the application of ZT may help conserve soil moisture and temperature. In the groves applied with ZT and organic manure (CK), C_Sap was found in significant correlations with environmental parameters, such as air temperature (T_A), air humidity (M_A), wind speed (S_W), soil water content at 0-5 cm depth（W_S5）and at >10-15 cm depth (W_S15). Treatment ZT was higher than CK in K, Ca, Fe, Mn and Cu content and in collection rate, content of protein, reducing sugar and amino acids and pH of the bleeding sap. Analysis of the bleeding sap from wine bamboo shows it contains 18 amino acids, among which GLU, ALA, and PRO are comparatively high in content. Therefore, the sap is rich in nutrient and hence a good raw material for development of a natural beverage. Application of ZT may prolong the sap collection period, increase sap yield and the contents of multi mineral elements, conventional nutrients and amino acids in the sap. So ZT, i. e. bamboo bio-char-based biomanure, may enjoy a bright future in application as a biomanure.

Key words: sympodial bamboo, bamboo biochar-based biomanure, mineral elements, amino acid, environmental factor explanation