玉米接种丛枝菌根真菌后对土壤铅污染的耐受效应

doi:10.19741/j.issn.1673-4831.2022.0314

Abstract

Abstract: The agronomic characters and physio-chemical indexes of plants under heavy metal pollution will be affected in varying degrees. Arbuscular mycorrhizal fungi (AMF) play an important role in plant response to abiotic stress due to their unique properties. In order to understand the tolerance effect of maize to Pb after symbiosis with arbuscular mycorrhizal fungi (AMF), the environment led by Pb pollution in the maize farmland was simulated using exogenous Pb(NO₃)₂ aging soil for 15 days. The effects of AMF inoculation (Funneliformis mosseae, Fm; Claroideoglomus etunicatum, Ce; Glomus versiforme, Gv) and no AMF inoculation (NM) on the growth of maize at different Pb concentrations (0, 170, 570, 970 mg·kg^-1) were investigated in pot experiment, and the changes of agronomic traits, antioxidant capacity and Pb content of maize inoculated with AMF were analyzed. The results show that the colonization rate of mycorrhizal under Pb contamination was 15.0%-36.0% higher than that without Pb contamination. The height and chlorophyll content of colonized maize were increased by 11.8%-26.8% and 3.9%-7.5%, respectively, and the fresh weight increased by 50%. The translocation factor decreased, meanwhile the Pb content in stem and leaf of maize decreased significantly, but for root it increased significantly (P＜0.05). Resistance analysis indicate that the formation of mycorrhiza inhibited the activity of SOD in maize leaves, but significantly increased the activity of SOD in maize roots, while the activity of POD in the leaves and roots increased. When the concentration of Pb was high, the activity of CAT in roots increased significantly (P＜0.05). The formation of mycorrhiza resulted in the decrease of proline content in maize roots and the increase of soluble protein content in maize leaves and roots (P＜0.05). Under the condition of Pb pollution, AMF inoculation increased the antioxidant capacity of maize, increased the content of soluble protein in maize leaves and roots, and improved the water retention capacity, so as to enhance the resistance of maize to Pb pollution and ensure the normal growth of maize in Pb-contaminated soil. This study provides a theoretical basis for the remediation of heavy metal contaminated soil in farmland and the safe production of crops.

Key words: maize, lead pollution, arbuscular mycorrhizal fungi, antioxidant enzyme, tolerance

CLC Number:

S513

YANG Yi-sen, SUN Chen-yu, MA Jun-qing, QIN Sheng-feng, ZHOU Xun-bo, HUANG Jing-hua. Effect of Arbuscular Mycorrhizal Fungi (AMF) on Maize Tolerance to Lead (Pb) Contaminated Soil[J]. Journal of Ecology and Rural Environment, 2023, 39(10): 1316-1322.

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Effect of Arbuscular Mycorrhizal Fungi (AMF) on Maize Tolerance to Lead (Pb) Contaminated Soil

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