Abstract: In order to screen out functional strains of bacteria that can be applied directly to promote composting of fresh swine manure,eight strais of bacteria that may possibly promote fermentation of fresh swine manure were isolated from swine manure compost,and indentified with the 16S rDNA molecular biological method as Bacillus subtilis,Enterobacter sp.,Bacillus licheniformis,Brodetella sp.,Nocardiopsis sp.,and Microbacterium sp..Effects of the strains promoting composting of fresh swine manure was investigated separately.Results show that Bacillus subtilis and Enterobacter sp. could raise temperature of the pile of manure under composting and extend duration of high temperature,thus accelerating decomposition of organic matter,reducing pH of the pile at the intial fermentation stage,reducing of C/N ratio and water content in the compost,and increasing relative content of total nitrogen.As a result,composting of the manure was accelerated.It is,therefore,held that Bacillus subtilis and Enterobacter sp. are the two optimal strains for use in composting fresh swine manure.When Bacillus licheniformis was used,the temperature of the pile should be properly lowered at the initial stage of the composting so as to improve its effect on composting.Nocardiopsis sp. could be used as supplementary zymogen in composting.As Bordetella sp. could be uesd to raise temperature of the pile under fermentation,but did not show any significant effects on other indices,they are not recommended as zymogens for use in composting fresh swine manure.

Key words: composting, 16S rDNA, identification, swine manure, bacteria