生物炭对土壤微生物的影响研究进展

doi:10.11934/j.issn.1673-4831.2016.01.010

摘要/Abstract

摘要：

生物炭是有机材料在厌氧条件下热解而成的产物。近年来,生物炭因在碳固定、土壤改良和作物产量提高等方面具有较大的应用潜力而引起国内外学者的广泛关注。作为一类新型的土壤改良剂,它能提高土壤有机碳含量及阳离子交换量(CEC),改善土壤保肥持水性能,有益于土壤微生物活动,同时还可吸附抑制对土壤微生物生长有毒的化感物质,为土壤微生物提供有利的栖息场所。但生物炭的效应与生物炭的特性、用量、土壤类型及肥力有关。笔者从生物炭对土壤微生物的影响及其作用机制出发,概述了不同生物质材料及热解温度对生物炭理化性质的影响及生物炭对土壤微生物丰度、群落结构和活性影响的研究进展。未来应重点从生物炭的特性、生物炭与微生物交互作用及生物炭的环境修复等方面深入研究,客观评价生物炭对土壤微生物的作用。

关键词: 生物炭, 土壤, 微生物丰度, 微生物群落结构, 微生物活性

Abstract:

Biochar is a product of thermal degradation of organic materials in the absence of air(pyrolysis), and is distinguished from charcoal by its use as a soil amendment. In recent years, biochar has been attracting more and more attention worldwide because of its great potential for carbon sequestration, soil amelioration and higher crop yield. As a new type of soil amendment, biochar can increase soil organic carbon content, cation exchange capacity and soil water-holding capacity, which are beneficial to microbial activity. Moreover, biochar can also adsorb toxic allelopathic substances in soil and provide a favorable habitat for microorganism. But the effects depend on biochar properties, application rates, soil types and soil fertility. Effects of type of parent material and pyrolysis temperature on physicochemical properties of biochar and effects of the biochars on abundance, community structure and activity of the soil microbes in the soil were generalized. It is recommended that future studies should go further in depth on biochar properties, interaction between biochar and soil microbes and effects of biochar remedying the environment so as to evaluate objectively the effects of biochar amendment on soil microbes.

Key words: biochar, soil, microbial abundance, microbial community structure, microbial activity

中图分类号:

S154.3

饶霜, 卢阳, 黄飞, 蔡一霞, 蔡昆争. 生物炭对土壤微生物的影响研究进展[J]. 生态与农村环境学报, 2016, 32(1): 53-59.

RAO Shuang, LU Yang, HUANG Fei, CAI Yi-xia, CAI Kun-zheng. A Review of Researches on Effects of Biochars on Soil Microorganisms[J]. Journal of Ecology and Rural Environment, 2016, 32(1): 53-59.

参考文献

[1] MAJOR J,RONDON M,MOLINA D,et al.Maize Yield and Nutrition During 4 Years After Biochar Application to a Colombian Savanna Oxisol[J].Plant and Soil,2010,333(1/2):117-128.
[2] ELAD Y,CYTRYN E,HAREL Y M,et al.The Biochar Effect:Plant Resistance to Biotic Stresses[J].Phytopathologia Mediterranea,2011,50(3):335-349.
[3] GERMANO M G,CANNAVAN F D S,MENDES L W,et al.Functional Diversity of Bacterial Genes Associated With Aromatic Hydrocarbon Degradation in Anthropogenic Dark Earth of Amazonia[J].Pesquisa Agropecuaria Brasileira,2012,47(5):654-664.
[4] OLESZCZUK P,JOSKO I,FUTA B,et al.Effect of Pesticides on Microorganisms,Enzymatic Activity and Plant in Biochar-Amended Soil[J].Geoderma,2014,214/215(2):10-18.
[5] PIETIKAINEN J,KⅡKKILA O,FRITZE H.Charcoal as a Habitat for Microbes and Its Effect on the Microbial Community of the Underlying Humus[J].Oikos,2000,89(2):231-242.
[6] KIM J,SPAROVEK G,LONGO R M,et al.Bacterial Diversity of Terra Preta and Pristine Forests From the Western Amazon[J].Soil Biology and Biochemistry,2007,39(2):684-690.
[7] GOMEZ J D,DENEF K,STEWART C E,et al.Biochar Addition Rate Influences Soil Microbial Abundance and Activity in Temperate Soils[J].European Journal of Soil Science,2014,65(1):28-39.
[8] STEINER C,GLASER B,TEIXEIRA W G,et al.Nitrogen Retention and Plant Uptake on a Highly Weathered Central Amazonian Ferralsol Amended With Compost and Charcoal[J].Journal of Plant Nutrition and Soil Science,2008,171(6):893-899.
[9] RILLIG M C,MUMMEY D L.Mycorrhizas and Soil Structure[J].New Phytologist,2006,171(1):41-53.
[10] 佟雪娇,李九玉,姜军,等.添加农作物秸秆炭对红壤吸附Cu(Ⅱ)的影响[J].生态与农村环境学报,2011,27(5):37-41.
[11] 甘文君,何跃,张孝飞,等.秸秆生物炭修复电镀厂污染土壤的效果和作用机理初探[J].生态与农村环境学报,2012,28(3):305-309.
[12] SUN K,GAO B,RO K S,et al.Assessment of Herbicide Sorption by Biochars and Organic Matter Associated With Soil and Sediment[J].Environmental Pollution,2012,163(4):167-173.
[13] GOMEZ-EYLES J L,SIZMUR T,COLLINS C D,et al.Effects of Biochar and the Earthworm Eisenia fetida on the Bioavailability of Polycyclic Aromatic Hydrocarbons and Potentially Toxic Elements[J].Environmental Pollution,2011,159(2):616-622.
[14] LEHMANN J,RILLIG M C,THIES J,et al.Biochar Effects on Soil Biota:A Review[J].Soil Biology and Biochemistry,2011,43(9):1812-1836.
[15] KEILUWEIT M,NICO P S,JOHNSON M G,et al.Dynamic Molecular Structure of Plant Biomass-Derived Black Carbon(Biochar)[J].Environmental Science and Technology,2010,44(4):1247-1253.
[16] HALE S E,LEHMANN J,RUTHERFORD D,et al.Quantifying the Total and Bioavailable Polycyclic Aromatic Hydrocarbons and Dioxins in Biochars[J].Environmental Science and Technology,2012,46(5):2830-2838.
[17] 仓龙,朱向东,汪玉,等.生物质炭中的污染物含量及其田间施用的环境风险预测[J].农业工程学报,2012,28(15):163-167.
[18] KEILUWEIT M,KLEBER M,SPARROW M A,et al.Solvent-Extractable Polycyclic Aromatic Hydrocarbons in Biochar:Influence of Pyrolysis Temperature and Feedstock[J].Environmental Science and Technology,2012,46(17):9333-9341.
[19] AHMAD M,LEE S S,DOU X,et al.Effects of Pyrolysis Temperature on Soybean Stover and Peanut Shell-Derived Biochar Properties and TCE Adsorption in Water[J].Bioresource Technology,2012,118(8):536-544.
[20] GASKIN J W,STEINER C,HARRIS K,et al.Effect of Low-Temperature Pyrolysis Conditions on Biochar for Agricultural Use[J].Transactions of the Asabe,2008,51(6):2061-2069.
[21] HMID A,MONDELLI D,FIORE S,et al.Production and Characterization of Biochar From Three-Phase Olive Mill Waste Through Slow Pyrolysis[J].Biomass and Bioenergy,2014,71:330-339.
[22] YAO F X,ARBESTAIN M C,VIRGEL S,et al.Simulated Geochemical Weathering of a Mineral Ash-Rich Biochar in a Modified Soxhlet Reactor[J].Chemosphere,2010,80(7):724-732.
[23] CHAN K Y,VAN ZWIETEN L,MESZAROS I,et al.Using Poultry Litter Biochars as Soil Amendments[J].Soil Research,2008,46(5):437-444.
[24] JOSEPH S D,CAMPS-ARBESTAIN M,LIN Y,et al.An Investigation Into the Reactions of Biochar in Soil[J].Soil Research,2010,48(7):501-515.
[25] MAKOTO K,TAMAI Y,KIM Y S,et al.Buried Charcoal Layer and Ectomycorrhizae Cooperatively Promote the Growth of Larix gmelinii Seedlings[J].Plant and Soil,2010,327(1/2):143-152.
[26] SOLAIMAN Z M,BLACKWELL P,ABBOTT L K,et al.Direct and Residual Effect of Biochar Application on Mycorrhizal Root Colonisation,Growth and Nutrition of Wheat[J].Soil Research,2010,48(7):546-554.
[27] WARNOCK D D,MUMMEY D L,MCBRIDE B,et al.Influences of Non-Herbaceous Biochar on Arbuscular Mycorrhizal Fungal Abundances in Roots and Soils:Results From Growth-Chamber and Field Experiments[J].Applied Soil Ecology,2010,46(3):450-456.
[28] WARNOCK D D,LEHMANN J,KUYPER T W,et al.Mycorrhizal Responses to Biochar in Soil:Concepts and Mechanisms[J].Plant and Soil,2007,300(1/2):9-20.
[29] CHEN J,LIU X,LI L,et al.Consistent Increase in Abundance and Diversity but Variable Change in Community Composition of Bacteria in Topsoil of Rice Paddy Under Short Term Biochar Treatment Across Three Sites From South China[J].Applied Soil Ecology,2015,91:68-79.
[30] ANDERSON C R,CONDRON L M,CLOUGH T J,et al.Biochar Induced Soil Microbial Community Change:Implications for Biogeochemical Cycling of Carbon,Nitrogen and Phosphorus[J].Pedobiologia,2011,54(5/6):309-320.
[31] KHODADAD C L M,ZIMMERMAN A R,GREEN S J,et al.Taxa-Specific Changes in Soil Microbial Community Composition Induced by Pyrogenic Carbon Amendments[J].Soil Biology and Biochemistry,2011,43(2):385-392.
[32] O'NEILL B,GROSSMAN J,TSAI M T,et al.Bacterial Community Composition in Brazilian Anthrosols and Adjacent Soils Characterized Using Culturing and Molecular Identification[J].Microbial Ecology,2009,58(1):23-35.
[33] TAKETANI R G,TSAI S M.The Influence of Different Land Uses on the Structure of Archaeal Communities in Amazonian Anthrosols Based on 16S rRNA and amoA Genes[J].Microbial Ecology,2010,59(4):734-743.
[34] DOMENE X,MATTANA S,HANLEY K,et al.Medium-Term Effects of Corn Biochar Addition on Soil Biota Activities and Functions in a Temperate Soil Cropped to Corn[J].Soil Biology and Biochemistry,2014,72:152-162.
[35] LUO Y,DURENKAMP M,DE NOBILI M,et al.Microbial Biomass Growth,Following Incorporation of Biochars Produced at 350℃ or 700℃,in a Silty-Clay Loam Soil of High and Low pH[J].Soil Biology and Biochemistry,2013,57:513-523.
[36] THIESSEN S,GLEIXNER G,WUTZLER T,et al.Both Priming and Temperature Sensitivity of Soil Organic Matter Decomposition Depend on Microbial Biomass:An Incubation Study[J].Soil Biology and Biochemistry,2013,57:739-748.
[37] FARRELL M,KUHN T K,MACDONALD L M,et al.Microbial Utilisation of Biochar-Derived Carbon[J].Science of the Total Environment,2013,465(6):288-297.
[38] AMELOOT N,SLEUTEL S,CASE S D C,et al.C Mineralization and Microbial Activity in Four Biochar Field Experiments Several Years After Incorporation[J].Soil Biology and Biochemistry,2014,78:195-203.
[39] AMELOOT N,DE NEVE S,JEGAJEEVAGAN K,et al.Short-Term CO₂ and N₂O Emissions and Microbial Properties of Biochar Amended Sandy Loam Soils[J].Soil Biology and Biochemistry,2013,57:401-410.
[40] SMITH J L,COLLINS H P,BAILEY V L.The Effect of Young Biochar on Soil Respiration[J].Soil Biology and Biochemistry,2010,42(12):2345-2347.
[41] JONES D L,ROUSK J,EDWARDS-JONES G,et al.Biochar-Mediated Changes in Soil Quality and Plant Growth in a Three Year Field Trial[J].Soil Biology and Biochemistry,2012,45:113-124.
[42] QUILLIAM R S,MARSDEN K A,GERTLER C,et al.Nutrient Dynamics,Microbial Growth and Weed Emergence in Biochar Amended Soil Are Influenced by Time Since Application and Reapplication Rate[J].Agriculture Ecosystems and Environment,2012,158(1):192-199.
[43] SPOKAS K A,NOVAK J M,STEWART C E,et al.Qualitative Analysis of Volatile Organic Compounds on Biochar[J].Chemosphere,2011,85(5):869-882.
[44] 匡崇婷,江春玉,李忠佩,等.添加生物质炭对红壤水稻土有机碳矿化和微生物生物量的影响[J].土壤,2012,44(4):570-575.
[45] SANTOS F,TORN M S,BIRD J A.Biological Degradation of Pyrogenic Organic Matter in Temperate Forest Soils[J].Soil Biology and Biochemistry,2012,51:115-124.
[46] ROUSK J,BROOKES P C,BAATH E.Contrasting Soil pH Effects on Fungal and Bacterial Growth Suggest Functional Redundancy in Carbon Mineralization[J].Applied and Environmental Microbiology,2009,75(6):1589-1596.
[47] 袁金华,徐仁扣.稻壳制备的生物质炭对红壤和黄棕壤酸度的改良效果[J].生态与农村环境学报,2010,26(5):472-476.
[48] LEHMANN J.Bio-Energy in the Black[J].Frontiers in Ecology and the Environment,2007,5(7):381-387.
[49] CHINTALA R,SCHUMACHER T E,KUMAR S,et al.Molecular Characterization of Biochars and Their Influence on Microbiological Properties of Soil[J].Journal of Hazardous Materials,2014,279:244-256.
[50] STEWART C E,ZHENG J,BOTTE J,et al.Co-Generated Fast Pyrolysis Biochar Mitigates Green-House Gas Emissions and Increases Carbon Sequestration in Temperate Soils[J].Global Change Biology Bioenergy,2013,5(Suppl.2):153-164.
[51] GUL S,WHALEN J K,THOMAS B W,et al.Physico-Chemical Properties and Microbial Responses in Biochar-Amended Soils:Mechanisms and Future Directions[J].Agriculture,Ecosystems and Environment,2015,206:46-59.
[52] CHENG C,LEHMANN J,THIES J E,et al.Oxidation of Black Carbon by Biotic and Abiotic Processes[J].Organic Geochemistry,2006,37(11):1477-1488.
[53] ANYIKA C,ABDUL M Z,IBRAHIM Z,et al.The Impact of Biochars on Sorption and Biodegradation of Polycyclic Aromatic Hydrocarbons in Soils:A Review[J].Environmental Science and Pollution Research,2015,22(5):3314-3341.
[54] KASOZI G N,ZIMMERMAN A R,NKEDI-KIZZA P,et al.Catechol and Humic Acid Sorption Onto a Range of Laboratory-Produced Black Carbons(Biochars)[J].Environmental Science and Technology,2010,44(16):6189-6195.
[55] 袁敏,姜军,赵安珍,等.4种温度条件下制备的稻草炭对环丙氨嗪的吸附特征[J].生态与农村环境学报,2012,28(6):712-717.
[56] MATSUHASHI M,PANKRUSHINA A N,ENDOH K,et al.Studies on Carbon Material Requirements for Bacterial Proliferation and Spore Germination under Stress Conditions:A New Mechanism Involving Transmission of Physical Signals[J].Journal of Bacteriology,1995,177(3):688-693.
[57] AKIYAMA K,MATSUZAKI K,HAYASHI H.Plant Sesquiterpenes Induce Hyphal Branching in Arbuscular Mycorrhizal Fungi[J].Nature,2005,435(7043):824-827.
[58] POLLOCK M R.The Growth of H.Pertussis on Media Without Blood[J].British Journal of Experimental Pathology,1947,28(4):295-307.
[59] HOCKADAY W C,GRANNAS A M,KIM S,et al.The Transformation and Mobility of Charcoal in a Fire-Impacted Watershed[J].Geochimica et Cosmochimica Acta,2007,71(14):3432-3445.
[60] SAMONIN V V,ELIKOVA E E.A Study of the Adsorption of Bacterial Cells on Porous Materials[J].Microbiology,2004,73(6):696-701.
[61] CHENG C,LEHMANN J,ENGELHARD M H.Natural Oxidation of Black Carbon in Soils:Changes in Molecular Form and Surface Charge Along a Climosequence[J].Geochimica et Cosmochimica Acta,2008,72(6):1598-1610.
[62] RIVERA-UTRILLA J,BAUTISTA-TOLEDO I,FERRO-GARCIA M A,et al.Activated Carbon Surface Modifications by Adsorption of Bacteria and Their Effect on Aqueous Lead Adsorption[J].Journal of Chemical Technology and Biotechnology,2001,76(12):1209-1215.
[63] QUILLIAM R S,GLANVILLE H C,WADE S C,et al.Life in the "Charosphere":Does Biochar in Agricultural Soil Provide a Significant Habitat for Microorganisms?[J].Soil Biology and Biochemistry,2013,65:287-293.