浮床栽培鱼腥草对吉富罗非鱼血清免疫因子的影响

doi:10.11934/j.issn.1673-4831.2017.10.012

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (10): 950-954.doi: 10.11934/j.issn.1673-4831.2017.10.012

浮床栽培鱼腥草对吉富罗非鱼血清免疫因子的影响

郑尧^1,2, 胡庚东¹, 裘丽萍¹, 赵志祥², 宋超¹, 范立民¹, 孟顺龙¹, 徐跑^1,2, 陈家长^1,2,3

1. 中国水产科学研究院淡水渔业研究中心/农业部长江下游渔业生态环境监测中心/农业部水产品质量安全环境因子风险评估实验室(无锡), 江苏无锡 214081;
2. 南京农业大学无锡渔业学院, 江苏无锡 214081;
3. 农业部水产品质量安全控制重点实验室, 北京 100141

收稿日期:2016-09-06 出版日期:2017-10-25 发布日期:2017-11-24
通讯作者: 徐跑,E-mail:xup@ffrc.cn;陈家长,E-mail:chenjz@ffrc.cn E-mail:xup@ffrc.cn;chenjz@ffrc.cn
作者简介:郑尧(1986-),男,安徽安庆人,助理研究员,博士,研究方向为渔业生态环境保护。E-mail:zhengy@ffrc.cn
基金资助:
国家科技支撑计划（2015BAD13B03）；现代农业产业技术体系专项（CARS-46）；中央级科研院所基本科研业务费（2015JBFR03）

Effects of Cultivation of Houttuynia cordata on Floating Beds on Serumal Immune Factors of GIFT Tilapia

ZHENG Yao^1,2, HU Geng-dong¹, QIU Li-ping¹, ZHAO Zhi-xiang², SONG Chao¹, FAN Li-min¹, MENG Shun-long¹, XU Pao^1,2, CHEN Jia-zhang^1,2,3

1. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Fishery Eco-Evironment Monitoring Center of Lower Reaches of Yangtze River, Ministry of Agriculture/Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Environmental Factors(Wuxi), Ministry of Agriculture, Wuxi 214081, China;
2. Wuxi Fishery College of Nanjing Agricultural University, Wuxi 214081, China;
3. Key Laboratory of Quality and Safety Control for Aquatic Products, Ministry of Agriculture, Beijing 100141, China

Received:2016-09-06 Online:2017-10-25 Published:2017-11-24

摘要/Abstract

摘要：

为探究构建鱼腥草-罗非鱼共生体系能否对罗非鱼（Oreochromis niloticus）产生免疫增强效应，研究池塘中浮床栽培鱼腥草（Houttuynia cordata）（种植面积分别为0、5%、10%和15%）对不同免疫反应阶段吉富罗非鱼血清免疫因子的影响，测定免疫识别阶段免疫球蛋白（IgM）和表皮生长因子（EGF），炎症反应阶段相关因子干扰素-γ（IFN-γ）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）和白细胞介素-8（IL-8），以及效应阶段金属硫蛋白（MT）和转铁蛋白（TRF）等指标。结果表明，10%鱼腥草处理组吉富罗非鱼血清EGF、IL-8、IL-10、TNF-α和TRF含量显著高于其他处理组和对照组，5%鱼腥草处理组吉富罗非鱼血清IgM、IFN-γ含量显著高于其他处理组和对照组，且没有造成血清MT含量的降低。吉富罗非鱼养殖池塘种植鱼腥草（5%）能显著提高其不同免疫反应阶段血清免疫因子的活性。

关键词: 生物浮床, 鱼腥草, 免疫因子, 吉富罗非鱼

Abstract:

To explore whether the symbiont of Houttuynia cordata (0, 5%, 10% and 15% in plant area) and GIFT tilapia may enhance immunological effects of serumal immune factors of the fish at different phases via pond cultivation of the plants on floating beds, immunoglobulin M (IgM), epidermal growth factor (EGF); iterferon-γ (IFN-γ), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8); metallothionein (MT) and transferrin (TRF) of the fish was measured. Results show that the fish in the 10% group were significantly higher than those in any other groups in serumal EGF, IL-8, IL-10, TNF-α and TRF, and that the fish in the 5% group were significantly higher than those in any other groups in IgM and IFN-γ without showing any adverse effect on MT in the fish. Apparently, planting Houttuynia cordata up to 5% in area in the fish pond of GIFT tilapia can significantly enhance activities of serumal immune factors at different phases of the immune reaction.

Key words: biological floating bed, Houttuynia cordata, immune factor, Oreochromis niloticus

中图分类号:

X954

郑尧, 胡庚东, 裘丽萍, 赵志祥, 宋超, 范立民, 孟顺龙, 徐跑, 陈家长. 浮床栽培鱼腥草对吉富罗非鱼血清免疫因子的影响[J]. 生态与农村环境学报, 2017, 33(10): 950-954.

ZHENG Yao, HU Geng-dong, QIU Li-ping, ZHAO Zhi-xiang, SONG Chao, FAN Li-min, MENG Shun-long, XU Pao, CHEN Jia-zhang. Effects of Cultivation of Houttuynia cordata on Floating Beds on Serumal Immune Factors of GIFT Tilapia[J]. Journal of Ecology and Rural Environment, 2017, 33(10): 950-954.

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浮床栽培鱼腥草对吉富罗非鱼血清免疫因子的影响

Effects of Cultivation of Houttuynia cordata on Floating Beds on Serumal Immune Factors of GIFT Tilapia

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