营养盐与四环素对苦草生理生化特性的联合影响

doi:10.11934/j.issn.1673-4831.2017.11.010

生态与农村环境学报 ›› 2017, Vol. 33 ›› Issue (11): 1029-1034.doi: 10.11934/j.issn.1673-4831.2017.11.010

营养盐与四环素对苦草生理生化特性的联合影响

丁程成, 崔益斌, 杭小帅, 凌梦丹, 李维新

环境保护部南京环境科学研究所, 江苏南京 210042

收稿日期:2017-02-10 出版日期:2017-11-25 发布日期:2017-11-24
通讯作者: 崔益斌,E-mail:cyb@nies.org E-mail:cyb@nies.org
作者简介:丁程成(1980-),女,安徽蒙城人,副研究员,博士,主要从事水污染生物修复研究。E-mail:dcc@nies.org
基金资助:
国家自然科学基金（31572623）；中央级公益性科研院所基本科研业务专项（20160212）

Joint Effects of Nutrient Salts and Tetracycline Hydrochloride on Physio-Biochemistry of Vallisneria natans

DING Cheng-cheng, CUI Yi-bin, HANG Xiao-shuai, LING Meng-dan, LI Wei-xin

Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China

Received:2017-02-10 Online:2017-11-25 Published:2017-11-24

摘要/Abstract

摘要：

通过室内模拟，研究不同氮磷营养水平（中营养、富营养和超富营养）和不同质量浓度（0、0.1、0.2、0.5 mg·L^-1）四环素复合水体中，沉水植物苦草（Vallisneria natans）的可溶性蛋白、过氧化物酶（POD）活性、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量以及叶绿素（Chl）含量的变化响应。结果表明，四环素和氮磷联合作用对可溶性蛋白、SOD、Chl-a和Chl-b的影响显著，超富营养组和0.5 mg·L^-1四环素联合作用对苦草可溶性蛋白含量影响最显著，其含量为同一营养组中CK的47.4%；超富营养组和0.2 mg·L^-1四环素的联合作用对SOD活性有显著增强作用，其SOD活性为同一营养组中CK的1.45倍；中营养组和0.5 mg·L^-1四环素联合作用对苦草Chl-a、Chl-b含量的影响最显著，其含量分别是同一营养组中CK的75.7%和64.6%。

关键词: 营养盐, 四环素, 联合作用, 生理生化

Abstract:

An in-laboratory experiment was carried out to simulate Vallisneria natans growing in wastewaters different in eutrophication degree (moderate, high and super-high) and in tetracycline hydrochloride (TH) contamination degree (0, 0.1, 0.2 and 0.5 mg·L^-1). Leaves of the plants were sampled 30 days after the initiation of the experiment for analysis of activities of peroxidase (POD) and superoxide dismutase (SOD) and contents of malondialdehyde (MDA), soluble proteins and chlorophyll for responses of the plants to changes in the environment. Results show that TH, N and P worked together affecting soluble protein, SOD, Chl-a and Chl-b in the plant significantly. In the super-eutrophication group, addition of 0.5 mg·L^-1 TH affected soluble protein the most significantly, which dropped by 47.4% in content vs that in CK of the group (0 mg·L^-1 TH). In the super-eutrophication group, addition of 0.2 mg·L^-1 TH enhanced SOD activity significantly or by 1.45 times vs that CK. In the group of moderate eutrophication, addition of 0.5 mg·L^-1 TH affected the concentrations of Chl-a and Chl-b the most significantly, which was increased by 75.7% and 64.6%, respectively, vs that in CK of the group.

Key words: nutrient salt, tetracycline hydrochloride, joint effect, physio biochemistry

中图分类号:

X171

丁程成, 崔益斌, 杭小帅, 凌梦丹, 李维新. 营养盐与四环素对苦草生理生化特性的联合影响[J]. 生态与农村环境学报, 2017, 33(11): 1029-1034.

DING Cheng-cheng, CUI Yi-bin, HANG Xiao-shuai, LING Meng-dan, LI Wei-xin. Joint Effects of Nutrient Salts and Tetracycline Hydrochloride on Physio-Biochemistry of Vallisneria natans[J]. Journal of Ecology and Rural Environment, 2017, 33(11): 1029-1034.

参考文献

[1] 高立红,史亚利,厉文辉,等.抗生素环境行为及其环境效应研究进展[J].环境化学,2013,32(9):1619-1633.[GAO Li-hong,SHI Ya-li,LI Wen-hui,et al.Environmental Behavior and Impacts of Antibiotics[J].Environmental Chemisrty,2013,32(9):1619-1633.]
[2] 刘建康.东湖生态学研究(二)[M].北京:科学出版社,1995:286-302.[LIU Jian-kang.Ecological Study of Donghu LakeⅡ[M].Beijing:Science Press,1995:286-302.]
[3] 焦少俊,窦艳艳,陈良燕,等.四环素对苦草(Vallisneria natans)生长及细胞超微结构的影响[J].环境化学,2008,27(3):335-338.[JIAO Shao-jun,DOU Yan-yan,CHEN Liang-yan,et al.Effect of Aqueous Tetracycline on Vallisneria natans in Growth and Ultrastructure[J].Environmental Chemistry,2008,27(3):335-338.]
[4] MOORE K A,WETZEL R L.Seasonal Variations in Elgrass (Zostera marina L.) Responses to Nutrient Enrichment and Reduced Light Availability in Experimental Ecosystems[J].Journal of Experimental Marine Biology and Ecology,2000,244(1):1-28.
[5] 叶春,邹国燕,付子轼,等.总氮浓度对3种沉水植物生长的影响[J].环境科学学报,2007,27(5):739-746.[YE Chun,ZOU Guo-yan,FU Zi-shi,et al.Responses of Three Submersed Macrophytes to Total Nitrogen Supply[J].Acta Scientiae Circumstantiae,2007,27(5):739-746.]
[6] BOWLER C,VAN C M,INZÉ D.Superoxide Dismutase and Stress Tolerance[J].Annual Review of Plant Molecular Biology,1992,43:83-116.
[7] 王萍,张兴旭,赵晓静,等.重金属胁迫对醉马草生长及生理生化指标的影响[J].草业科学,2013,31(6):1080-1086.[WANG Ping,ZHANG Xing-xu,ZHAO Xiao-jing,et al.Effects of Three Metal Ions on Growth and Physio-Biochemical Response of Achnatherum inebrians[J].Pratacultural Science,2013,31(6):1080-1086.]
[8] 许木启,黄玉瑶.受损水域生态系统恢复与重建研究[J].生态学报,1998,18(5):547-558.[XU Mu-qi,HUANG Yu-yao.Restoration and Reestablishment of the Damaged Ecosystem of Inland Waters[J].Acta Ecologica Sinica,1998,18(5):547-558.]
[9] 宋睿,姜锦林,耿金菊,等.不同浓度铵态氮对苦草的生理影响[J].中国环境科学,2011,31(3):448-453.[SONG Rui,JIANG Jin-lin,GENG Jin-ju,et al.Physiological Effect of Vallisneria natans L.Under Different Concentrations of Ammonia[J].China Environmental Science,2011,31(3):448-453.]
[10] 许宽,刘波,王国祥,等.苦草(Vallisneria spiralis)对城市缓流河道黑臭底泥理化性质的影响[J].环境科学,2013,34(7):2642-2649.[XU Kuan,LIU Bo,WANG Guo-xiang,et al.Influence of Vallisneria spiralis on the Physicochemical Properties of Black-Odor Sediment in Urban Sluggish River[J].Environmental Science,2013,34(7):2642-2649.]
[11] 阿丽腾·吐尔孙哈力,巴特尔·巴克,何方,等.沙尘胁迫对库尔勒香梨叶片可溶性蛋白和叶绿素含量的影响[J].山东农业科学,2016,48(9):69-72.[ALTEN·TUERSUNHALI,BATUR·BAKE,HE Fang,et al.Effects of Dust Stress on Leaf Soluble Protein and Chlorophyll Content of Korla Fragrant Pear[J].Shangdong Agricultural Sciences,2016,48(9):69-72.]
[12] LARCHER W.Physiological Plant Ecology[M].4th ed.New York,USA:Springer,2003:86-92.
[13] 胡珍珍,崔益斌,李梅,等.水体氮磷营养盐水平与氯霉素浓度复合水体对苦草的生理生化影响[J].环境科学,2015,36(9):3248-3254.[HU Zhen-zhen,CUI Yi-bin,LI Mei,et al.Physiological Effect of Vallisneria natans Under Different Concentrations of Nitrogen,Phosphorus and Chloramphenicol[J].Environmental Science,2015,36(9):3248-3254.]
[14] 吴志刚,沈洪艳,高吉喜,等.青土霉素废水出水对锦鲤肝脏抗氧化酶活性和丙二醛含量的影响[J].江西农业大学学报,2013,35(3):587-592.[WU Zhi-gang,SHEN Hong-yan,GAO Ji-xi,et al.Effects of Wastewater Effluent of Penicillin and Oxytetracycline on SOD,CAT Activies and MDA Content in Liver Tissue of Cyprinus carpio[J].Acta Agriculture Universitatis Jiangxiensis,2013,35(3):587-592.]
[15] 鲁敏,裴翡翡,宁静,等.4种湿地植物受污水胁迫生理生化特性影响的相关性研究[J].山东建筑大学学报,2011,26(5):416-419.[LU Min,PEI Fei-fei,NING Jing,et al.Correlation Study on Effects of Wastewater Duress on Physiological and Biochemical Characteristics of 4 Wetland Plants[J].Journal of Shangdong Jianzhu University,2011,26(5):416-419.]
[16] 蔡炜,宋玉芝.水体营养盐质量浓度对苦草光合荧光特性的影响[J].环境科学研究,2009,22(8):907-913.[CAI Wei,SONG Yu-zhi.Effect of Water Nutrient Concentration on Photosynthetic Fluorescence Characteristics of Vallisneria natans[J].Research of Environmental Sciences,2009,22(8):907-913.]
[17] 卢广超,许建新,薛立,等.干旱胁迫下4种常用植物幼苗的光合和荧光特性综合评价[J].生态学报,2013,33(24):7872-7881.[LU Guang-chao,XU Jian-xin,XUE Li,et al.Comprehensive Evaluation on Photosynthetic and Fluorescence Characteristics in Seedlings of 4 Drought Resistance Species[J].Acta Ecologica Sinica,2013,33(24):7872-7881.]
[18] 周启星,程云,张倩茹,等.复合污染生态毒理效应的定量关系分析[J].中国科学:C辑,2003,33(6):566-573.[ZHOU Qi-xing,CHENG Yun,ZHANG Qian-ru,et al.Quantitative Ecotoxicological Effect of Compound Pollutant[J].Science in China:Series C,2003,33(6):566-573.]
[19] 华涛,周启星.Cd-Zn对草鱼(Ctenopharyngodon idellus)的联合毒性及对肝脏超氧化物歧化酶(SOD)活性的影响[J].环境科学学报,2009,29(3):600-606.[HUA Tao,ZHOU Qi-xing.Joint Toxic Effects of Cd and Zn on Grass Carp(Ctenopharyngodon idellus) and SOD Activity in Livers[J].Acta Scientiae Circumstantiae,2009,29(3):600-606.]
[20] KASAI K,KANNO T,ENDO Y,et al.Guanosine Tetra and Pentaphosphate Synthase Activity in Chloroplasts of a Higher Plant:Association With 70S Ribosomes and Inhibition by Tetracyline[J].Nucleic Acids Research,2004,32(19):5732-5741.

营养盐与四环素对苦草生理生化特性的联合影响

Joint Effects of Nutrient Salts and Tetracycline Hydrochloride on Physio-Biochemistry of Vallisneria natans

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价

[1]	刘述锡, 孙淑艳, 王真良, 陈素梅, 温泉. 飞云江与鳌江口海域夏、秋季环境因子与浮游生物的分布变化[J]. 生态与农村环境学报, 2014, 30(4): 430-437.
[2]	史新, 徐应明, 谢忠雷, 孙约兵, 胡发志. 膨润土对镉胁迫下水稻幼苗生理生化特性的影响[J]. 生态与农村环境学报, 2012, 28(6): 687-693.
[3]	朱向东, 王玉军, 孙瑞娟, 周东美. 溶液酸度对四环素类物质光降解和光催化降解速率的影响[J]. 生态与农村环境学报, 2012, 28(6): 742-745.
[4]	常龙飞, 王晓龙, 李恒鹏, 胡锋. 巢湖典型低山丘陵区不同土地利用类型壤中流养分流失特征[J]. 生态与农村环境学报, 2012, 28(5): 511-517.
[5]	王博, 叶春, 李春华, 江源, 孔祥龙. 初春苦草腐解过程中营养盐释放过程剂规律[J]. 生态与农村环境学报, 2012, 28(2): 171-175.
[6]	姜蕾;陈书怡;尹大强. 四环素对铜绿微囊藻光合作用和抗氧化酶活性的影响[J]. 生态与农村环境学报, 2010, 26(6): 564-567.
[7]	周骏, 王长永, 陈建群. 转基因植物入侵性评价指标初探[J]. 生态与农村环境学报, 2003, 19(2): 61-64.
[8]	周红卫, 施国新, 徐勤松, 谷魏, 徐楠, 曾晓敏, 张晓兰. Cr⁶⁺和Cr³⁺对水花生几种生理生化指标的影响比较[J]. 生态与农村环境学报, 2002, 18(4): 35-40.
[9]	施铭, 刘宏纲. 淀山湖网箱养鱼及其对水质的影响[J]. 生态与农村环境学报, 1989, 5(3): 7-11.
[10]	张雁秋. 凤眼莲的容量对净化、利用的影响[J]. 生态与农村环境学报, 1989, 5(1): 40-43,47.