基于三维荧光光谱结合平行因子法的蘑菇湖上覆水溶解性有机质特征分析

doi:10.19741/j.issn.1673-4831.2018.0600

摘要/Abstract

摘要：

溶解性有机质（dissolved organic matter，DOM）广泛存在于水体、土壤和沉积物中，在自然系统有机物向无机物的转换过程中起重要作用。DOM作为载体可影响污染物迁移转化，作为碳源和营养元素又为微生物提供能量。为探讨湖泊水体中DOM组成、来源和腐殖化程度，以蘑菇湖上覆水为例，采用三维荧光光谱（3DEEM）结合平行因子法（PARAFAC）分析蘑菇湖上覆水DOM组分，利用荧光指数（FI）、自生源指标（BIX）和腐殖化指数（HIX）等光谱指数研究DOM来源和腐殖化程度，并通过FI与最大荧光强度（F_max）的相关性解释DOM种类和特征。结果表明：（1）蘑菇湖上覆水DOM包含4种组分，其中C1和C4为富里酸物质，C2为类蛋白中色氨酸物质，C3为腐殖酸物质；（2）蘑菇湖上覆水DOM以微生物内源代谢产物为主，生物可利用性较高，腐殖化程度较低；（3）各组分F_max与FI间均呈正相关关系，其中富里酸与FI之间呈显著正相关（r=0.89，P<0.01）。这表明蘑菇湖上覆水DOM主要为含羰基、羟基等活性官能团的相对分子质量小，且腐殖化、芳香性和分子缩合度较低的类富里酸物质。

关键词: 溶解性有机质(DOM), 三维荧光光谱, 平行因子分析, 光谱指数, 蘑菇湖

Abstract:

Dissolved organic matter is an essential effect on the conversion exchange between organic matter and inorganic matter in nature environment, which is widely distributed in water,soil and sediments. As a pollutants carrier,it affects the migration and transformation of contaminants,while it also provides carbon source and nutrients for microorganisms. For exploring the composition, source and humification degree of DOM in Moguhu Lake,the components of DOM in the overlying water was analyzed by using three-dimensional fluorescence spectroscopy combined with parallel factors. The source and humification degree were studied by means of spectral index (FI, HIX, BIX), and the correlation and relationship between FI and F_max were used to explain the type and characteristics of DOM. The results show that in Moguhu Lake,DOM of overlying water contains four components, which are two represent fulvic acid substances for C1 and C4, tryptophan in the protein C2 and humic acid C4. Secondly,the DOM is mainly composed from microbial endogenous metabolites with high bioavailability and low degree of humification. Positive correlation was found between each component F_max and FI,and a significant positive correlation existed between fulvic acid and FI. According to previous studies, in Moguhu Lake,the primary component of DOM is fulvic acid-like substance,which contains a reactive functional group such as a carbonyl group or a hydroxyl group with small molecular,and with characteristics of a low degree of humification,aromaticity,and low molecular condensation.

Key words: dissolved organic matter(DOM), three-dimensional fluorescence spectrum, paralle factor analysis, spectral index, Moguhu Lake

中图分类号:

X524
O657.3

张广彩, 于会彬, 徐泽华, 宋永会, 韩美. 基于三维荧光光谱结合平行因子法的蘑菇湖上覆水溶解性有机质特征分析[J]. 生态与农村环境学报, 2019, 35(7): 933-939.

ZHANG Guang-cai, YU Hui-bin, XU Ze-hua, SONG Yong-hui, HAN Mei. The Characteristics of Dissolved Organic Matter in the Overlying Water of Moguhu Lake Based on Three Dimensional Fluorescence Spectrum and Parallel Factor Method[J]. Journal of Ecology and Rural Environment, 2019, 35(7): 933-939.

参考文献 31

[1]	NKANSAH K,ADEDIPE O,DAWSON-ANDOH B,et al. Determination of Concentration of ACQ Wood Preservative Components by UV-Visible Spectroscopy Coupled With Multivariate Data Analysis[J]. Chemometrics and Intelligent Laboratory Systems,2015,147:157-166.
[2]	张春华,黄廷林,方开凯,等. 同温混合初期主库区沉积物间隙水DOM的光谱特征:以周村水库为例[J]. 中国环境科学, 2016,36(10):3048-3055. [ZHANG Chun-hua,HUANG Tinglin,FANG Kai-kai,et al. Spectral Characteristics of DOM in Sediment Interstitial Water of the Main Reservoir Area During the Initial Stage of Isothermal Mixing:A Case Study of Zhoucun Reservoir[J]. China Environmental Science,2016,36(10):3048-3055.]
[3]	READER H E,STEDMON C A,NIELSEN N J,et al. Mass and UV-Visible Spectral Fingerprints of Dissolved Organic Matter:Sources and Reactivity[J]. Frontiers in Marine Science,2015, 2:88.
[4]	XU P D,ZHU J,FU Q L,et al. Structure and Biodegradability of Dissolved Organic Matter From Ultisol Treated With Long Term Fertilizations[J]. Journal of Soils and Sediments,2018,18(5):1865-1872.
[5]	YAN M Q,KORSHIN G,WANG D S,et al. Characterization of Dissolved Organic Matter Using High-Performance Liquid Chromatography (HPLC)-Size Exclusion Chromatography (SEC) With a Multiple Wavelength Absorbance Detector[J]. Chemosphere,2012,87(8):879-885.
[6]	叶琳琳,孔繁翔,史小丽,等. 富营养化湖泊溶解性有机碳生物可利用性研究进展[J]. 生态学报,2014,34(4):779-788.[YE Lin-lin,KONG Fan-xiang,SHI Xiao-li,et al. The Bioavailability of Dissolved Organic Carbon in the Eutrophic Lakes[J]. Acta Ecologica Sinica,2014,34(4):779-788.]
[7]	李帅东,张明礼,杨浩,等. 昆明松华坝库区表层土壤溶解性有机质(DOM)的光谱特性[J]. 光谱学与光谱分析,2017,37(4):1183-1188.[LI Shuai-dong,ZHANG Ming-li,YANG Hao,et al. Spectroscopic Characteristics of Dissolved Organic Matter From Top Soils on Songhuaba Reservoir in Kunmimg[J]. Spectroscopy and Spectral Analysis,2017,37(4):1183-1188.]
[8]	张博,王书航,姜霞,等. 太湖五里湖水体悬浮物中水溶性有机质(WSOM)的荧光光谱组分鉴别及其与氮形态的关系[J]. 湖泊科学,2018,30(1):102-111. [ZHANG Bo,WANG Shuhang,JIANG Xia,et al. Identification of WSOM Fluorescence Spectral Components in Suspended Solids and Correlation Analysis With Nitrogen Forms of Lake Wuli,Lake Taihu[J]. Journal of Lake Sciences,2018,30(1):102-111.]
[9]	PRAISE S,ITO H,AN Y,et al. Dissolved Organic Matter Characteristics Along Sabo Dammed Streams Based on Ultraviolet Visible and Fluorescence Spectral Properties[J]. Environmental Monitoring and Assessment,2018,190(3):146.
[10]	牛天浩,周振,胡大龙,等. 污水处理厂污泥水溶解性有机物的光谱特性分析[J]. 环境科学,2016,37(4):1460-1466.[NIU Tian-hao,ZHOU Zhen,HU Da-long,et al. Spectral Characteristics of Dissolved Organic Matters in Reject Water From Wastewater Treatment Plants[J]. Environmental Science,2016, 37(4):1460-1466.]
[11]	王静,吴丰昌,王立英,等. 超滤、三维荧光光谱与高效体积排阻色谱联合表征地表水环境中溶解有机质的性质[J]. 环境科学,2008,29(11):3027-3034.[WANG Jing,WU Feng chang,WANG Li-ying,et al. Combining Ultrafiltration,Fluorescence Spectroscopy and HPSEC to Characterize Dissolved Organic Matter in Surface Waters[J]. Environmental Science,2008, 29(11):3027-3034.]
[12]	BIRDWELL J E,ENGEL A S. Characterization of Dissolved Organic Matter in Cave and Spring Waters Using UV-Vis Absorbance and Fluorescence Spectroscopy[J]. Organic Geochemistry,2010,41(3):270-280.
[13]	何伟,白泽琳,李一龙,等. 溶解性有机质特性分析与来源解析的研究进展[J]. 环境科学学报,2016,36(2):359-372.[HE Wei,BAI Ze-lin,LI Yi-long,et al. Advances in the Characteristics Analysis and Source Identification of the Dissolved Organic Matter[J]. Acta Scientiae Circumstantiae,2016,36(2):359-372.]
[14]	祝鹏,廖海清,华祖林,等. 平行因子分析法在太湖水体三维荧光峰比值分析中的应用[J]. 光谱学与光谱分析,2012,32(1):152-156. [ZHU Peng,LIAO Hai-qing,HUA Zu-lin,et al. Parallel Factor Analysis as an Analysis Technique for the Ratio of Three-Dimensional Fluorescence Peak in Taihu Lake[J]. Spectroscopy and Spectral Analysis,2012,32(1):152-156.]
[15]	MANGALGIRI K P,TIMKO S A,GONSIOR M,et al. PARAFAC Modeling of Irradiation-and Oxidation-Induced Changes in Fluorescent Dissolved Organic Matter Extracted From Poultry Litter[J]. Environmental Science & Technology,2017,51(14):8036-8047.
[16]	BURDIGE D J,KLINE S W,CHEN W H. Fluorescent Dissolved Organic Matter in Marine Sediment Pore Waters[J]. Marine Chemistry,2004,89(1/2/3/4):289-311.
[17]	HERNÁNDEZ-SÁNCHEZ N,LLEÓ L,AMMARI F,et al. Fast Fluorescence Spectroscopy Methodology to Monitor the Evolution of Extra Virgin Olive Oils Under Illumination[J]. Food and Bioprocess Technology,2017,10(5):949-961.
[18]	OSBURN C L,HANDSEL L T,MIKAN M P,et al. Fluorescence Tracking of Dissolved and Particulate Organic Matter Quality in a River-Dominated Estuary[J]. Environmental Science & Technology,2012,46(16):8628-8636.
[19]	王书航,王雯雯,姜霞,等. 基于三维荧光光谱-平行因子分析技术的蠡湖CDOM分布特征[J]. 中国环境科学,2016,36(2):517-524.[WANG Shu-hang,WANG Wen-wen,JIANG Xia,et al. Distribution of Chromophoric Dissolved Organic Matter in Lihu Lake Using Excitation-Emission Matrix Fluorescence and Parallel Factor Analysis[J]. China Environmental Science, 2016,36(2):517-524.]
[20]	蔡文良,许晓毅,杜娴,等. 嘉陵江重庆段DOM三维荧光光谱的平行因子分析[J]. 环境科学研究,2012,25(3):276-281. [CAI Wen-liang,XU Xiao-yi,DU Xian,et al. Parallel Factor Analysis With EEM on Dissolved Organic Matter in Chongqing Section of Jialing River[J]. Research of Environmental Sciences,2012,25(3):276-281.]
[21]	MCKNIGHT D M,BOYER E W,WESTERHOFF P K,et al. Spectrofluorometric Characterization of Dissolved Organic Matter for Indication of Precursor Organic Material and Aromaticity[J]. Limnology and Oceanography,2001,46(1):38-48.
[22]	JOHNSON M S,COUTO E G,ABDO M,et al. Fluorescence Index as an Indicator of Dissolved Organic Carbon Quality in Hydrologic Flowpaths of Forested Tropical Watersheds[J]. Biogeochemistry,2011,105(1/2/3):149-157.
[23]	HUGUET A,VACHER L,RELEXANS S,et al. Properties of Fluorescent Dissolved Organic Matter in the Gironde Estuary[J]. Organic Geochemistry,2009,40(6):706-719.
[24]	OHNO T. Fluorescence Inner-Filtering Correction for Determining the Humification Index of Dissolved Organic Matter[J]. Environmental Science & Technology,2002,36(4):742-746.
[25]	OHNO T,CHOROVER J,OMOIKE A,et al. Molecular Weight and Humification Index as Predictors of Adsorption for Plant-and Manure-Derived Dissolved Organic Matter to Goethite[J]. European Journal of Soil Science,2007,58(1):125-132.
[26]	HE W,HUR J. Conservative Behavior of Fluorescence EEM PARAFAC Components in Resin Fractionation Processes and Its Applicability for Characterizing Dissolved Organic Matter[J]. Water Research,2015,83:217-226.
[27]	BAKER A,CURRY M. Fluorescence of Leachates From Three Contrasting Landfills[J]. Water Research,2004,38(10):2605-2613.
[28]	COBLE P G. Marine Optical Biogeochemistry:The Chemistry of Ocean Color[J]. Chemical Reviews,2007,107(2):402-418.
[29]	郭卫东,黄建平,洪华生,等. 河口区溶解有机物三维荧光光谱的平行因子分析及其示踪特性[J]. 环境科学,2010,31(6):1419-1427. [GUO Wei-dong,HUANG Jian-ping,HONG Hua-sheng,et al. Resolving Excitation Emission Matrix Spectroscopy of Estuarine CDOM With Parallel Factor Analysis and Its Application in Organic Pollution Monitoring[J]. Environmental Science,2010,31(6):1419-1427.]
[30]	NIE Z Y,WU X D,HUANG H M,et al. Tracking Fluorescent Dissolved Organic Matter in Multistage Rivers Using EEM PARAFAC Analysis:Implications of the Secondary Tributary Remediation for Watershed Management[J]. Environmental Science and Pollution Research,2016,23(9):8756-8769.
[31]	D'ORAZIO V,TRAVERSA A,SENESI N. Forest Soil Organic Carbon Dynamics as Affected by Plant Species and Their Corresponding Litters:A Fluorescence Spectroscopy Approach[J]. Plant and Soil,2014,374(1/2):473-484.