滇池湖滨湿地不同挺水植物区沉积物细菌群落结构特征

doi:10.19741/j.issn.1673-4831.2019.0182

生态与农村环境学报 ›› 2020, Vol. 36 ›› Issue (3): 390-398.doi: 10.19741/j.issn.1673-4831.2019.0182

滇池湖滨湿地不同挺水植物区沉积物细菌群落结构特征

寄博华^1,2, 李玮^1,3, 常军军^1,3, 李璇^1,2, 司光正^1,2, 何芳^1,2, 陈金全^1,3

1. 云南大学生态学与环境学院, 高原湖泊生态与治理研究院, 云南昆明 650091;
2. 云南大学国际河流与生态安全研究院, 云南昆明 650091;
3. 云南省高原山地生态与退化环境修复重点实验室, 云南昆明 650091

收稿日期:2019-03-25 出版日期:2020-03-25 发布日期:2020-03-25
通讯作者: 常军军, 陈金全 E-mail:changjunjun@ynu.edu.cn;chengjinquan@ynu.edu.cn
作者简介:寄博华(1996-),男,陕西宝鸡人,硕士生,研究方向为水环境生态修复。E-mail:jibohua@qq.com
基金资助:
国家自然科学基金（31700411）；云南省应用基础研究计划项目（2017FB090，2018FD007）；云南省高原山地生态与退化环境修复重点实验室开放基金（2018DG005）

Characteristics of Bacterial Community Structure in Sediments With Different Emergent Plants in Dianchi Lakeside Wetland

JI Bo-hua^1,2, LI Wei^1,3, CHANG Jun-jun^1,3, LI Xuan^1,2, SI Guang-zheng^1,2, HE Fang^1,2, CHEN Jin-quan^1,3

1. Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Science, Yunnan University, Kunming 650091, China;
2. Institute of International Rivers and Eco-security, Yunnan University, Kunming 650091, China;
3. Yunnan Key Laboratory for Plateau Mountain Ecology and Restoration of Degraded Environments, Yunnan University, Kunming 650091, China

Received:2019-03-25 Online:2020-03-25 Published:2020-03-25

摘要/Abstract

摘要： 滇池湖滨湿地在削减入湖面源污染和维持生态多样性等方面起着重要作用。通过高通量测序技术考察解析滇池斗南湿地菖蒲（Acorus calamus）、芦苇（Phragmites communis）和美人蕉（Canna indica）3种代表性挺水植物根区沉积物的细菌群落结构特征，并结合冗余分析考察环境因子对细菌群落结构的影响。结果表明：门水平上相对丰度较大的细菌为变形菌门（Proteobacteria）、绿弯菌门（Chloroflexi）、放线菌门（Actinobacteria）和酸杆菌门（Acidobacteria）。属水平上相对丰度较大的是厌氧绳菌科（Anaerolineaceae）下的未知属和假单胞菌（Pseudomonas）。菖蒲区沉积物细菌群落丰富度和多样性均最大，芦苇区细菌群落丰富度大于美人蕉区，但多样性小于美人蕉区。相同植物不同采样区沉积物细菌群落结构较相似，但不同植物区菌群结构差异较大，表明植物种类对细菌群落特征具有显著影响。相较于硝态氮和铵态氮含量，速效磷和有机质含量对细菌群落α和β多样性影响更大。

关键词: 滇池, 湖滨湿地, 芦苇, 美人蕉, 菖蒲, 细菌群落结构

Abstract: Wetlands constructed beside Dianchi Lake play important roles in reducing non-point source pollution and maintaining the ecological diversity. In this study, high-throughput sequencing technology was used to reveal the bacterial community structures in the sediments grown with three representative emergent plants, Acorus calamus, Phragmites communis and Canna indica in Dounan Wetland beside Dianchi Lake. The results show that the bacteria occupying relatively high abundance at the phylum level were Proteobacteria, Chloroflexi, Actinobacteria and Acidobacteria. At the genus level, the dominant bacteria were the unknown genus belonging to Anaerolineaceae and Pseudomonas. The richness and diversity of the bacterial communities in sediment with Acorus calamus were the largest. The bacterial richness in sediment with Phragmites communis was higher than that with Canna indica, but the diversity was lower. The bacterial community structure in sediments from different sampling points with the same macrophyte was similar, but was greatly different in those with different macrophytes, indicating that plant species had a significant effect on sediment bacterial community characteristics. The contents of available phosphorus and organic matter had a greater effect on α and β diversities of the sediment bacterial communities compared with nitrate and ammonium contents.

Key words: Dianchi Lake, lakeside wetland, Phragmites communis, Canna indica, Acorus calamus, bacterial community structure

中图分类号:

X832

寄博华, 李玮, 常军军, 李璇, 司光正, 何芳, 陈金全. 滇池湖滨湿地不同挺水植物区沉积物细菌群落结构特征[J]. 生态与农村环境学报, 2020, 36(3): 390-398.

JI Bo-hua, LI Wei, CHANG Jun-jun, LI Xuan, SI Guang-zheng, HE Fang, CHEN Jin-quan. Characteristics of Bacterial Community Structure in Sediments With Different Emergent Plants in Dianchi Lakeside Wetland[J]. Journal of Ecology and Rural Environment, 2020, 36(3): 390-398.

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滇池湖滨湿地不同挺水植物区沉积物细菌群落结构特征

Characteristics of Bacterial Community Structure in Sediments With Different Emergent Plants in Dianchi Lakeside Wetland

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