紫球藻对沼液废水中氨氮、铜离子及抗生素磺胺二甲嘧啶的处理效果

doi:10.19741/j.issn.1673-4831.2019.0829

摘要/Abstract

摘要： 微藻处理沼液废水是一项污水资源化处理的生物技术，将富含胞外多糖的紫球藻（Porphyridium cruentum）作为研究对象，以实际沼液废水中氨氮、铜离子（Cu²⁺）和抗生素磺胺二甲嘧啶浓度为对照，分别设置不同浓度上述污染物考察其对紫球藻生长的影响，并测定紫球藻对沼液废水中氨氮、Cu²⁺和抗生素磺胺二甲嘧啶的富集效果。结果表明，培养10 d后，在正常培养基以及ρ（氨氮）分别为50、500和2 000 mg·L^-1培养基中紫球藻生物量分别为1.67、1.74、0.85和0.68 g·L^-1；在正常培养基以及ρ（Cu²⁺）分别为0.5、1.0和2.0 mg·L^-1培养基中，紫球藻生物量分别为2.36、1.81、1.83和1.58 g·L^-1；在正常培养基以及ρ（抗生素）分别为5、10、20、100和200 mg·L^-1培养基中，紫球藻生物量分别为1.60、1.18、1.42、1.30、0.98和0.88 g·L^-1。此外，在最佳氨氮、Cu²⁺及磺胺二甲嘧啶浓度条件下紫球藻对各污染物去除率分别为73.2%、54.3%和56.9%。氨氮为紫球藻的生长提供了一定量营养盐，促进紫球藻生长；Cu²⁺和高浓度磺胺二甲嘧啶则抑制紫球藻生长。紫球藻富含胞外多糖的特性为沼液废水中难处理污染物Cu²⁺和抗生素等的富集去除提供了新的思路。

关键词: 紫球藻, 沼液, 氨氮, Cu²⁺, 抗生素, 磺胺二甲嘧啶

Abstract: Swine wastewater treatment by microalgae is the biotechnology for the resource utilization of wastewater. The microalgae Porphyridium cruentum, which is rich in exopolysaccharide is selected as the object in this study. The effect of NH₃-N, Cu²⁺, and antibiotic sulfadiazine from swine wastewater on the growth of Porphyridium cruentum was investigated. The study aims to investigate the effects of different conditions of pollutants on the growth of Porphyridium cruentum and determine the enrichment effects of the strain on NH₃-N, Cu²⁺, and antibiotics sulfadiazine. The results show that the biomass of Porphyridium cruentum was 1.74, 0.85 and 0.68 g·L^-1, respectively in the culture with NH₃-N concentration of 50, 500 and 2 000 mg·L^-1; with Cu²⁺ concentration of 0.5, 1.0 and 2.0 mg·L^-1, the biomass of Porphyridium cruentum was 1.81, 1.83 and 1.58 g·L^-1, respectively; and with antibiotic sulfadiazine concentration of 5, 10, 20, 100 and 200 mg·L^-1, the biomass of Porphyridium cruentum was 1.18, 1.42, 1.30, 0.98 and 0.88 g·L^-1, respectively. Moreover, the removal rates of NH₃-N, Cu²⁺, and sulfamethazine by Porphyridium cruentum were 73.2%, 54.3% and 56.9%, respectively under the optimal concentrations of the mentioned pollutants. The results indicate that ammonia nitrogen could provide nutrition for the microalgae Porphyridium cruentum and promote growth of the microalgae. However, Cu²⁺ and high concentrations of antibiotic sulfadiazine could inhibit the growth of Porphyridium cruentum. The abundant exopolysaccharide from Porphyridium cruentum has provided a new idea for the bioconcentration and removal of refractory pollutant metal Cu²⁺ or antibiotics in swine wastewater.

Key words: Porphyridium cruentum, swine wastewater, ammonia nitrogen, copper, antibiotics, sulfadiazine

中图分类号:

X703

彭瑶, 胡政宇, 肖鹏, 许智慧, 常婷, 程鹏飞. 紫球藻对沼液废水中氨氮、铜离子及抗生素磺胺二甲嘧啶的处理效果[J]. 生态与农村环境学报, 2020, 36(9): 1185-1191.

PENG Yao, HU Zheng-yu, XIAO Peng, XU Zhi-hui, CHANG Ting, CHENG Peng-fei. Effect of Ammonia Nitrogen, Copper and Antibiotics Sulfadimidine in Swine Wastewater on the Growth of Porphyridium cruentum[J]. Journal of Ecology and Rural Environment, 2020, 36(9): 1185-1191.

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