畜禽养殖场污水处理系统中诺氟沙星抗性菌的筛选及其抗性基因分析

doi:10.19741/j.issn.1673-4831.2019.0343

生态与农村环境学报 ›› 2020, Vol. 36 ›› Issue (2): 250-256.doi: 10.19741/j.issn.1673-4831.2019.0343

畜禽养殖场污水处理系统中诺氟沙星抗性菌的筛选及其抗性基因分析

赵翰斌, 陈鑫, 武广哲, 周纯淳, 刘莉莉

华东理工大学资源与环境工程学院/国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237

收稿日期:2019-05-14 发布日期:2020-03-03
通讯作者: 刘莉莉,E-mail:lilyliu@ecust.edu.cn E-mail:lilyliu@ecust.edu.cn
作者简介:赵翰斌(1995-),男,上海市人,硕士生,研究方向为环境微生物。E-mail:1058508314@qq.com
基金资助:
国家水体污染控制与治理科技重大专项（2017ZX07202006）

Isolation of Norfloxacin Resistant Bacteria and Analysis of Its Antibiotic Resistance Genes From the Wastewater Treatment System of a Livestock Farm

ZHAO Han-bin, CHEN Xin, WU Guang-zhe, ZHOU Chun-chun, LIU Li-li

School of Resource and Environmental Engineering/State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, China

Received:2019-05-14 Published:2020-03-03

摘要/Abstract

摘要： 畜禽养殖场污水处理系统中抗生素抗性菌、抗性基因的富集和扩散对生态环境和人类健康具有潜在风险，因此有必要对畜禽养殖污水生物处理系统中的抗性菌、抗性基因的种类和遗传特性等进行研究。笔者从江苏某肉鹅养殖场污水处理系统的二沉池污泥样品中分离得到一株诺氟沙星抗性菌TY-1，根据其形态、生理生化特征及16S rRNA基因鉴定结果，该菌株属于鞘氨醇单胞菌属（Sphingomonas）。对鞘氨醇单胞菌属的全基因组分析表明，菌株TY-1含多种抗生素抗性蛋白，包括腺苷三磷酸结合盒转运蛋白（ABC transporter）、主要易化子超家族转运蛋白（MFS transporter）和细胞色素P450脱氢酶，与鞘氨醇单胞菌属的对应功能酶具有很高的同源性。对比鞘氨醇单胞菌属抗生素抗性基因的基因簇发现，这些抗性基因在鞘氨醇单胞菌属中普遍存在，表明其在该菌属中有一定的保守性。研究结果可为畜禽养殖场污水处理系统中含抗生素抗性基因的致病微生物的生态风险评估等相关研究提供参考。

关键词: 畜禽养殖场, 氟喹诺酮类抗生素, 鞘氨醇单胞菌, 抗生素抗性基因

Abstract: The accumulation and diffusion of antibiotic resistant bacteria and resistance genes in wastewater treatment systems of livestock farm poses potential hazards to ecological environment and human health. Therefore, it is necessary to investigate the types of resistant bacteria, resistance genes and genetic characteristics in the livestock wastewater treatment systems. In this study, a norfloxacin-resistant strain TY-1 was isolated from the sludge, which was collected from the secondary sedimentation tank of a meat goose farm in Jiangsu Province. The strain TY-1 was identified as Sphingomonas sp., according to the results of morphological, physiological and biochemical characteristics, and 16S rRNA gene sequence analysis. Based on the whole genome annotation of Sphingomonas sp., it was found that strain TY-1 had multiple antibiotic resistance genes (ARGs), including ABC transporter, MFS transporter and cytochrome P450 dehydrogenase. According to the results of gene cluster analysis, these ARGs were ubiquitous in genus of Sphingomonas. This study can provide a useful reference for the ecological risk assessment of pathogenic microorganisms containing antibiotic resistance genes in the wastewater treatment systems of livestock farm.

Key words: livestock farm, fluoroquinolones, Sphingomonas sp., antibiotic resistance genes

中图分类号:

X172

赵翰斌, 陈鑫, 武广哲, 周纯淳, 刘莉莉. 畜禽养殖场污水处理系统中诺氟沙星抗性菌的筛选及其抗性基因分析[J]. 生态与农村环境学报, 2020, 36(2): 250-256.

ZHAO Han-bin, CHEN Xin, WU Guang-zhe, ZHOU Chun-chun, LIU Li-li. Isolation of Norfloxacin Resistant Bacteria and Analysis of Its Antibiotic Resistance Genes From the Wastewater Treatment System of a Livestock Farm[J]. Journal of Ecology and Rural Environment, 2020, 36(2): 250-256.

参考文献

[1] LI C,LU J J,LIU J,et al.Exploring the Correlations Between Antibiotics and Antibiotic Resistance Genes in the Wastewater Treatment Plants of Hospitals in Xinjiang,China[J].Environmental Science and Pollution Research,2016,23(15):1-11.
[2] SU H C,YING G G,TAO R,et al.Class 1 and 2 Integrons,Sul Resistance Genes and Antibiotic Resistance in Escherichia coli Isolated From Dongjiang River,South China[J].Environmental Pollution,2012,169:42-49.
[3] ZHANG M,LIU Y S,ZHAO J L,et al.Occurrence,Fate and Mass Loadings of Antibiotics in Two Swine Wastewater Treatment Systems[J].Science of the Total Environment,2018,639:1421-1431.
[4] EVERAGE T J,BOOPATHY R,NATHANIEL R,et al.A Survey of Antibiotic-Resistant Bacteria in a Sewage Treatment Plant in Thibodaux,Louisiana,USA[J].International Biodeterioration & Biodegradation,2014,95:2-10.
[5] YUAN Q B,ZHAI Y F,MAO B Y,et al.Antibiotic Resistance Genes and,IntI1 Prevalence in a Swine Wastewater Treatment Plant and Correlation With Metal Resistance,Bacterial Community and Wastewater Parameters[J].Ecotoxicology and Environmental Safety,2018,161:251-259.
[6] CHEN B,HAO L J,GUO X Y,et al.Prevalence of Antibiotic Resistance Genes of Wastewater and Surface Water in Livestock Farms of Jiangsu Province,China[J].Environmental Science and Pollution Research,2015,22(18):13950-13959.
[7] MARTI E,HUERTA B,RODRÍGUEZ-MOZAZ S,et al.Characterization of Ciprofloxacin-Resistant Isolates From a Wastewater Treatment Plant and Its Receiving River[J].Water Research,2014,61:67-76.
[8] LI J R,WANG Y X,WANG X,et al.Intercalation and Adsorption of Ciprofloxacin by Layered Chalcogenides and Kinetics Study[J].Journal of Colloid and Interface Science,2015,453:69-78.
[9] GRAOUER-BACART M,SAYEN S,GUILLON E.Macroscopic and Molecular Approaches of Enrofloxacin Retention in Soils in Presence of Cu(Ⅱ)[J].Journal of Colloid and Interface Science,2013,408(1):191-199.
[10] ZHANG C L,QIAO G L,ZHAO F,et al.Thermodynamic and Kinetic Parameters of Ciprofloxacin Adsorption Onto Modified Coal Fly Ash From Aqueous Solution[J].Journal of Molecular Liquids,2011,163(1):53-56.
[11] ZHANG Q Q,YING G G,PAN C G,et al.Comprehensive Evaluation of Antibiotics Emission and Fate in the River Basins of China:Source Analysis,Multimedia Modeling,and Linkage to Bacterial Resistance[J].Environmental Science & Technology,2015,49(11):6772-6782.
[12] 赵晶,毕春娟,陈振楼,等.上海市崇明岛养殖场周边环境中氟喹诺酮类抗生素的含量特征[J].生态与农村环境学报,2017,33(2):120-126.[ZHAO Jing,BI Chun-juan,CHEN Zhen-lou,et al.Contents of Fluoroquinolone-Type Antibiotics in the Surroundings of Livestock Farms in Chongming Island of Shanghai[J].Journal of Ecology and Rural Environment,2017,33(2):120-126.]
[13] ZHAO L,DONG Y H,WANG H.Residues of Veterinary Antibiotics in Manures from Feedlot Livestock in Eight Provinces of China[J].Science of the Total Environment,2010,408(5):1069-1075.
[14] JUNG C M,HEINZE T M,STRAKOSHA R,et al.Acetylation of Fluoroquinolone Antimicrobial Agents by an Escherichia coli Strain Isolated From a Municipal Wastewater Treatment Plant[J].Journal of Applied Microbiology,2010,106(2):564-571.
[15] ZHANG T,DONG J,CHENG Y,et al.Genotypic Diversity,Antimicrobial Resistance and Biofilm-Forming Abilities of Campylobacter Isolated From Chicken in Central China[J].Gut Pathogens,2017,9(1):62.
[16] REDGRAVE L S,SUTTON S B,WEBBER M A,et al.Fluoroquinolone Resistance:Mechanisms,Impact on Bacteria,and Role in Evolutionary Success[J].Trends in Microbiology,2014,22(8):438-445.
[17] SATO T,YOKOTA S I,OKUBO T,et al.Contribution of the AcrAB-TolC Efflux Pump to High-Level Fluoroquinolone Resistance in Escherichia coli Isolated From Dogs and Humans[J].Journal of Veterinary Medical Science,2013,75(4):407-414.
[18] JIA Y,KHANAL S K,SHU H Y,et al.Ciprofloxacin Degradation in Anaerobic Sulfate-Reducing Bacteria (SRB) Sludge System:Mechanism and Pathways[J].Water Research,2018,136:64-74.
[19] ZHANEL G G,HOBAN D J,SCHUREK K,et al.Role of Efflux Mechanisms on Fluoroquinolone Resistance in Streptococcus pneumoniae and Pseudomonas aeruginosa[J].International Journal of Antimicrobial Agents,2004,24(6):529-535.
[20] ARCHUNDIA D,DUWIG C,LEHEMBER F,et al.Antibiotic Pollution in the Katari Subcatchment of the Titicaca Lake:Major Transformation Products and Occurrence of Resistance Genes[J].Science of the Total Environment,2017,576:671-682.
[21] 刘莉莉,汪烯烯,陈鑫,等.Methylobacterium sp.TP-1好氧降解四溴双酚A机理及其功能酶研究[J].环境科学学报,2018,38(11):4250-4258.[LIU Li-li,WANG Xi-xi,CHEN Xin,et al.Studies on Aerobic Degradation Mechanism of Tetrabromobisphenol A and Functional Enzymes of Methylobacterium sp. TP-1[J].Acta Scientiae Circumstantiae,2018,38(11):4250-4258.]
[22] WANG H B,SHEN Y,HU C,et al.Sulfadiazine/Ciprofloxacin Promote Opportunistic Pathogens Occurrence in Bulk Water of Drinking Water Distribution Systems[J].Environmental Pollution,2018,234:71-78.
[23] HEGYI H,GERSTEIN M.The Relationship Between Protein Structure and Function:A Comprehensive Survey With Application to the Yeast Genome[J].Journal of Molecular Biology,1999,288(1):147-164.
[24] BLACK P A,WARREN R M,LOUW G E,et al.Energy Metabolism and Drug Efflux in Mycobacterium tuberculosis[J].Antimicrobial Agents and Chemotherapy,2014,58(5):2491-2503.
[25] CHIEN J Y,YU C J,HSUEH P R.High Incidence of Fluoroquinolone Resistance and Effect of Efflux Pump Inhibitors on Moxifloxacin Resistance Among Mycobacterium tuberculosis Isolates Causing Urinary Tract Infection in Taiwan[J].International Journal of Antimicrobial Agents,2017,50(3):491-495.
[26] GAUR R,JEENA G,SHAH N,et al.High Density Linkage Mapping of Genomic and Transcriptomic SNPs for Synteny Analysis and Anchoring the Genome Sequence of Chickpea[J].Scientific Reports,2015,5(13387):1-11.

畜禽养殖场污水处理系统中诺氟沙星抗性菌的筛选及其抗性基因分析

Isolation of Norfloxacin Resistant Bacteria and Analysis of Its Antibiotic Resistance Genes From the Wastewater Treatment System of a Livestock Farm

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	张兰河, 王佳佳, 高敏, 仇天雷, 韩梅琳, 王旭明. 施用畜禽粪便有机肥土壤抗生素抗性基因污染状况[J]. 生态与农村环境学报, 2016, 32(4): 664-669.
[2]	叶小梅, 常志州, 陈欣, 黄红英, 马艳, 张建英. 畜禽养殖场排放物病原微生物危险性调查[J]. 生态与农村环境学报, 2007, 23(2): 66-70.
[3]	单正军. 加拿大畜牧业环境保护管理考察报告[J]. 生态与农村环境学报, 2000, 16(4): 61-62.