转基因作物安全性评价研究的文献计量可视化分析

doi:10.19741/j.issn.1673-4831.2021.0023

摘要/Abstract

摘要： 随着全球转基因作物种植面积持续不断的增加，转基因作物的生物和生态安全一直备受关注。为了解转基因作物生物安全性评价的全球研究动态和热点，笔者以Web of Science （WOS）和中国知网（CNKI）为检索数据库，利用Hiscite和Citespace软件分析了1999-2020年间的转基因作物研究文献。结果表明：（1） WOS发文量呈先升高后稳定的趋势，其中2018年发文量最高，为237篇；CNKI发文量呈先升高后下降的趋势，其中2014年发文量最高，为68篇；（2） WOS发文量排名前5位的国家分别为美国、中国、英国、德国和法国，发文量依次为1 246、615、342、272和260篇；（3） WOS发文量排名前5位的研究机构分别为中国农业科学院、法国农业科学研究院、美国孟山都公司、美国爱荷华州立大学和美国农业部农业研究组织，发文量依次为182、115、113、112和93篇；（4）该领域研究者最为关注的研究方向包括基因飘移、对非靶标生物的影响、靶标害虫抗性和食用安全性等，其中我国在转基因作物对非靶标生物影响方面的研究较多，而在基因飘移方面的研究较少；此外，WOS研究热点还涉及转基因大豆、转基因作物与传统作物的共存、转基因作物的环境风险等，CNKI研究热点还涉及转基因水稻、转基因育种与传统育种技术比较、转基因安全证书等。

关键词: 转基因作物, 安全评价, 文献计量分析, 数据库, 信息可视化

Abstract: With increasing area of the transgenic crops, the biological and ecological safety of the transgenic crops has become a focus topic. To keep track of the global research trends and hot spots of safety of the transgenic crops, relevant literatures from 1999 to 2020 were explored based on the data of Web of Science(WOS) and China National Knowledge Infrastructure(CNKI), and software Hiscite and Citespace were used to achieve bibliometrics visual analysis. The results show that(1) the number of literatures in WOS increased at first and then stabilized, with the highest number of 237 literatures in 2018; the number of literatures in CNKI increased at first and then decreased, with the highest number of 68 publications in 2014; (2) United States, China, United Kingdom, Germany and France were the top 5 countries for publication numbers in WOS, with 1 246, 615, 342, 272 and 260 literatures, respectively; (3) Chinese Academy of Agricultural Sciences, Institut National de la Recherche Agronomique, Monsanto Company, Iowa State University and U.S. Department of Agriculture-Agricultural Research Service were the top 5 institutions for publication numbers in WOS, with 182, 115, 113, 112 and 93 literatures respectively; (4) In this field, 4 topics were highly concerned by the researchers, including gene flow, effects on non-target organisms, resistance of target pest and food safety. Among them, the study on effects of transgenic crops on non-target organisms were abundant, but the studies on gene flow were relatively lacking. In China, In addition, research hotspots in WOS were transgenic soybean, coexistence of transgenic and traditional crops, and environmental harm of transgenic crops, and research hotspots in CNKI were transgenic rice, comparison of transgenic and traditional breeding techniques, safety certificates.

Key words: transgenic crops, safety assessment, bibliometric analysis, database, information visualization

中图分类号:

任振涛, 薛堃, 周宜君. 转基因作物安全性评价研究的文献计量可视化分析[J]. 生态与农村环境学报, 2021, 37(12): 1503-1513.

REN Zhen-tao, XUE Kun, ZHOU Yi-jun. Bibliometrics Visual Analysis of Literature on the Safety Assessment of Transgenic Crops[J]. Journal of Ecology and Rural Environment, 2021, 37(12): 1503-1513.

参考文献 61

[1]	BROOKES G, BARFOOT P.GM Crops:Global Socio-economic and Environmental Impacts 1996-2011[M].Dorchester, UK:PG Economics Ltd, 2013:13-14.
[2]	GARCIA-YI J, LAPIKANONTH T, VIONITA H, et al.What are the Socio-Economic Impacts of Genetically Modified Crops Worldwide? a Systematic Map Protocol[J].Environmental Evidence, 2014, 3(1):1-17.
[3]	CHEN C M, SONG M.Visualizing a Field of Research:A Methodology of Systematic Scientometric Reviews[J].PLoS One, 2019, 14(10):e0223994.
[4]	顾洪涛, 王筠.基于两种模型的学科发展趋势预测:以文献计量学为例[J].现代情报, 2013, 33(2):162-165.[GU Hong-tao, WANG Jun.A Forecast of the Development Tendency for Discipline Based on Two Models:Take Bibliometrics as an Example[J].Journal of Modern Information, 2013, 33(2):162-165.]
[5]	ROMEIS J, BARTSCH D, BIGLER F, et al.Assessment of Risk of Insect-Resistant Transgenic Crops to Nontarget Arthropods[J].Nature Biotechnology, 2008, 26(2):203-208.
[6]	STEWART C N, HALFHILL M D, WARWICK S I.Transgene Introgression from Genetically Modified Crops to Their Wild Relatives[J].Nature Reviews Genetics, 2003, 4(10):806-817.
[7]	TABASHNIK B E, GASSMANN A J, CROWDER D W, et al.Insect Resistance to Bt Crops:Evidence Versus Theory[J].Nature Biotechnology, 2008, 26(2):199-202.
[8]	CONKO G, KERSHEN D L, MILLER H, et al.A Risk-Based Approach to the Regulation of Genetically Engineered Organisms[J].Nature Biotechnology, 2016, 34(5):493-503.
[9]	ELLSTRAND N C, PRENTICE H C, HANCOCK J F.Gene Flow and Introgression from Domesticated Plants into Their Wild Relatives[J].Annual Review of Ecology and Systematics, 1999, 30(1):539-563.
[10]	HAYGOOD R, IVES A R, ANDOW D A.Consequences of Recurrent Gene Flow from Crops to Wild Relatives[J].Proceedings of Biological Sciences, 2003, 270(1527):1879-1886.
[11]	HALL L, TOPINKA K, HUFFMAN J, et al.Pollen Flow between Herbicide-resistant Brassica napusis the Cause of Multiple-resistant B.napus Volunteers1[J].Weed Science, 2000, 48(6):688-694.
[12]	ARNAUD J F, VIARD F, DELESCLUSE M, et al.Evidence for Gene Flow via Seed Dispersal from Crop to Wild Relatives in Beta vulgaris (Chenopodiaceae):Consequences for the Release of Genetically Modified Crop Species with Weedy Lineages[J].Proceedings Biological Sciences, 2003, 270(1524):1565-1571.
[13]	HEGDE S G, WAINES J G.Hybridization and Introgression between Bread Wheat and Wild and Weedy Relatives in North America[J].Crop Science, 2004, 44(4):1145-1155.
[14]	LU B R, YANG C.Gene Flow from Genetically Modified Rice to Its Wild Relatives:Assessing Potential Ecological Consequences[J].Biotechnology Advances, 2009, 27(6):1083-1091.
[15]	KURODA Y, KAGA A, TOMOOKA N, et al.Gene Flow and Genetic Structure of Wild Soybean (Glycine soja) in Japan[J].Crop Science, 2008, 48(3):1071-1079.
[16]	刘标, 薛堃, 刘来盘, 等.转EPSPS+PAT基因大豆向非转基因大豆的基因漂移研究[J].生态与农村环境学报, 2020, 36(3):367-373.[LIU Biao, XUE Kun, LIU Lai-pan, et al.Research on the Gene Flow from Transgenic EPSPS+PAT Soybean S4003.14 to Non-Transgenic Soybeans[J].Journal of Ecology and Rural Environment, 2020, 36(3):367-373.]
[17]	WARWICK S I, BECKIE H J, HALL L M.Gene Flow, Invasiveness, and Ecological Impact of Genetically Modified Crops[J].Annals of the New York Academy of Sciences, 2009, 1168(1):72-99.
[18]	ROMEIS J, RAYBOULD A, BIGLER F, et al.Deriving Criteria to Select Arthropod Species for Laboratory Tests to Assess the Ecological Risks from Cultivating Arthropod-Resistant Genetically Engineered Crops[J].Chemosphere, 2013, 90(3):901-909.
[19]	HANSEN J L C, OBRYCKI J J.Field Deposition of Bt Transgenic Corn Pollen:Lethal Effects on the Monarch Butterfly[J].Oecologia, 2000, 125(2):241-248.
[20]	SEARS M K, HELLMICH R L, STANLEY-HORN D E, et al.Impact of Bt Corn Pollen on Monarch Butterfly Populations:A Risk Assessment[J].PNAS, 2001, 98(21):11937-11942.
[21]	LU Y H, WU K M, JIANG Y Y, et al.Widespread Adoption of Bt Cotton and Insecticide Decrease Promotes Biocontrol Services[J].Nature, 2012, 487(7407):362-365.
[22]	SAXENA D, FLORES S, STOTZKY G.Bt Toxin is Released in Root Exudates from 12 Transgenic Corn Hybrids Representing Three Transformation Events[J].Soil Biology and Biochemistry, 2002, 34(1):133-137.
[23]	CARPENTER J E.Impact of GM Crops on Biodiversity[J].GM Crops, 2011, 2(1):7-23.
[24]	ZEILINGER A R, ANDOW D A, ZWAHLEN C, et al.Earthworm Populations in a Northern US Cornbelt Soil are not Affected by Long-Term Cultivation of Bt Maize Expressing Cry1Ab and Cry3Bb1 Proteins[J].Soil Biology and Biochemistry, 2010, 42(8):1284-1292.
[25]	洪鑫, 韩成, 孔帆, 等.抗虫-耐除草剂转基因玉米种植对根际土壤细菌和真菌群落的影响[J].生态与农村环境学报, 2020, 36(3):358-366.[HONG Xin, HAN Cheng, KONG Fan, et al.Effects of Insect-Resistant and Herbicide-Tolerant Transgenic Maize on Rhizospheric Bacterial and Fungal Communities[J].Journal of Ecology and Rural Environment, 2020, 36(3):358-366.]
[26]	郭佳惠, 李刚, 赵建宁, 等.抗旱和耐盐碱转基因棉花对土壤线虫群落的影响[J].生态与农村环境学报, 2018, 34(6):541-546.[GUO Jia-hui, LI Gang, ZHAO Jian-ning, et al.Effects of Cultivation of Drought-Resistance and Salt-tolerance Transgenic Cotton on Soil Nematode Community[J].Journal of Ecology and Rural Environment, 2018, 34(6):541-546.]
[27]	TABASHNIK B E, BRÉVAULT T, CARRIōRE Y.Insect Resistance to Bt Crops:Lessons from the First Billion Acres[J].Nature Biotechnology, 2013, 31(6):510-521.
[28]	TABASHNIK B E, VAN RENSBURG J B J, CARRIōRE Y.Field-Evolved Insect Resistance to Bt Crops:Definition, Theory, and Data[J].Journal of Economic Entomology, 2009, 102(6):2011-2025.
[29]	GOULD F.Sustainability of Transgenic Insecticidal Cultivars:Integrating Pest Genetics and Ecology[J].Annual Review of Entomology, 1998, 43:701-726.
[30]	LIU C, XIAO Y, LI X, et al.Cis-mediated Down-regulation of a Trypsin Gene Associated with Bt Resistance in Cotton Bollworm[J].Scientific Reports, 2014, 4:7219.
[31]	WEI J, GUO Y, LIANG G, et al.Cross-resistance and Interactions between Bt Toxins Cry1Ac and Cry2Ab Against the Cotton Bollworm[J].Scientific Reports, 2015, 5:7714.
[32]	JIN L, ZHANG H, LU Y, et al.Large-scale Test of the Natural Refuge Strategy for Delaying Insect Resistance to Transgenic Bt Crops[J].Nature Biotechnology, 2015, 33(2):169-174.
[33]	CELLINI F, CHESSON A, COLQUHOUN I, et al.Unintended Effects and Their Detection in Genetically Modified Crops[J].Food and Chemical Toxicology, 2004, 42(7):1089-1125.
[34]	AULRICH K, BÖHME H, DAENICKE R, et al.Genetically Modified Feeds in Animal Nutrition 1st Communication:Bacillus thuringiensis (Bt) Corn in Poultry, Pig and Ruminant Nutrition[J].Archiv Für Tierernaehrung, 2001, 54(3):183-195.
[35]	NOVAK W K, HASLBERGER A G.Substantial Equivalence of Antinutrients and Inherent Plant Toxins in Genetically Modified Novel Foods[J].Food and Chemical Toxicology, 2000, 38(6):473-483.
[36]	AUMAITRE A, AULRICH K, CHESSON A, et al.New Feeds from Genetically Modified Plants:Substantial Equivalence, Nutritional Equivalence, Digestibility, and Safety for Animals and the Food Chain[J].Livestock Production Science, 2002, 74(3):223-238.
[37]	VENNERIA E, FANASCA S, MONASTRA G, et al.Assessment of the Nutritional Values of Genetically Modified Wheat, Corn, and Tomato Crops[J].Journal of Agricultural and Food Chemistry, 2008, 56(19):9206-9214.
[38]	GOODMAN R E, VIETHS S, SAMPSON H A, et al.Allergenicity Assessment of Genetically Modified Crops:What Makes Sense?[J].Nature Biotechnology, 2008, 26(1):73-81.
[39]	HERMAN R A, PRICE W D.Unintended Compositional Changes in Genetically Modified (GM) Crops:20 Years of Research[J].Journal of Agricultural and Food Chemistry, 2013, 61(48):11695-11701.
[40]	DE CAMPOS B K, GALAZZI R M, DOS S B M, et al.Comparison of Generational Effect on Proteins and Metabolites in Non-Transgenic and Transgenic Soybean Seeds through the Insertion of the Cp4-EPSPS Gene Assessed by Omics-Based Platforms[J].Ecotoxicology and Environmental Safety, 2020, 202:110918.
[41]	XUE K, YANG J, LIU B, et al.The Integrated Risk Assessment of Transgenic Rice Oryza Sativa:A Comparative Proteomics Approach[J].Food Chemistry, 2012, 135(1):314-318.
[42]	AVIRON S, SANVIDO O, ROMEIS J, et al.Case-Specific Monitoring of Butterflies to Determine Potential Effects of Transgenic Bt-Maize in Switzerland[J].Agriculture, Ecosystems & Environment, 2009, 131(3/4):137-144.
[43]	CHEN Y, GAO Y J, ZHU H J, et al.Effects of Straw Leachates from Cry1C-Expressing Transgenic Rice on the Development and Reproduction of Daphnia magna[J].Ecotoxicology and Environmental Safety, 2018, 165:630-636.
[44]	YANG Y, ZHANG B, ZHOU X, et al.Toxicological and Biochemical Analyses Demonstrate the Absence of Lethal or Sublethal Effects of Cry1C-or Cry2A-Expressing Bt Rice on the Collembolan folsomia Candida[J].Frontiers in Plant Science, 2018, 9:131.
[45]	GUO Y Y, TIAN J C, SHI W P, et al.The Interaction of Two-Spotted Spider Mites, Tetranychus urticae Koch, with Cry Protein Production and Predation by Amblyseius andersoni (Chant) in Cry1Ac/Cry2Ab Cotton and Cry1F Maize[J].Transgenic Research, 2016, 25(1):33-44.
[46]	WANG Y Y, LI Y H, HUANG Z Y, et al.Toxicological, Biochemical, and Histopathological Analyses Demonstrating that Cry1C and Cry2A are not Toxic to Larvae of the Honeybee, Apis mellifera[J].Journal of Agricultural and Food Chemistry, 2015, 63(27):6126-6132.
[47]	BABENDREIER D, REICHHART B, ROMEIS J, et al.Impact of Insecticidal Proteins Expressed in Transgenic Plants on Bumblebee Microcolonies[J].Entomologia Experimentalis et Applicata, 2008, 126(2):148-157.
[48]	ÁLVAREZ-ALFAGEME F, PÁLINKÁS Z, BIGLER F, et al.Development of an Early-Tier Laboratory Bioassay for Assessing the Impact of Orally-Active Insecticidal Compounds on Larvae of Coccinella septempunctata (Coleoptera:Coccinellidae)[J].Environmental Entomology, 2012, 41(6):1687-1693.
[49]	ROMEIS J, MEISSLE M, NARANJO S E, et al.The End of a Myth-Bt (Cry1Ab) Maize does not Harm Green Lacewings[J].Frontiers in Plant Science, 2014, 5:391.
[50]	SU H H, TIAN J C, NARANJO S E, et al.Bacillus thuringiensis Plants Expressing Cry1Ac, Cry2Ab and Cry1F are not Toxic to the Assassin bug, Zelus renardii[J].Journal of Applied Entomology, 2015, 139(1/2):23-30.
[51]	MEISSLE M, ROMEIS J.The Web-Building Spider Theridion impressum (Araneae:Theridiidae) Is Not Adversely Affected by Bt Maize Resistant to Corn Rootworms[J].Plant Biotechnology Journal, 2009, 7(7):645-656.
[52]	李杰, 陈超美.CiteSpace:科技文本挖掘及可视化[M].北京:首都经济贸易大学出版社, 2016:149-150.
[53]	ISLAM N, BATES P D, MARIA JOHN K M, et al.Quantitative Proteomic Analysis of Low Linolenic Acid Transgenic Soybean Reveals Perturbations of Fatty Acid Metabolic Pathways[J].PROTEOMICS, 2019, 19(7):1800379.
[54]	GRAY E, ANCEV T, DRYNAN R.Coexistence of GM and Non-GM Crops with Endogenously Determined Separation[J].Ecological Economics, 2011, 70(12):2486-2493.
[55]	YAN S, YU J, HAN M, et al.Intercrops can Mitigate Pollen-mediated Gene Flow from Transgenic Cotton while Simultaneously Reducing Pest Densities[J].Science of the Total Environment, 2020, 711:134855.
[56]	RAYBOULD A, KILBY P, GRASER G.Characterising Microbial Protein Test Substances and Establishing Their Equivalence with Plant-produced Proteins for Use in Risk Assessments of Transgenic Crops[J].Transgenic Research, 2013, 22(2):445-460.
[57]	RAYBOULD A, TUTTLE A, SHORE S, et al.Environmental Risk Assessments for Transgenic Crops Producing Output Trait Enzymes[J].Transgenic Research, 2010, 19(4):595-609.
[58]	KEESE P K, ROBOLD A V, MYERS R C, et al.Applying a Weed Risk Assessment Approach to GM Crops[J].Transgenic Research, 2014, 23(6):957-969.
[59]	徐琳杰, 孙卓婧, 杨雄年, 等.科学视角下的转基因技术认知和发展[J].中国生物工程杂志, 2016, 36(4):30-34.[XU Lin-jie, SUN Zhuo-jing, YANG Xiong-nian, et al.Cognition and Development of Genetic Modification from Perspective of Sciences[J].China Biotechnology, 2016, 36(4):30-34.]
[60]	SINGH O V, GHAI S, PAUL D, et al.Genetically Modified Crops:Success, Safety Assessment, and Public Concern[J].Applied Microbiology and Biotechnology, 2006, 71(5):598-607.
[61]	KÖNIG A, COCKBURN A, CREVEL R W R, et al.Assessment of the Safety of Foods Derived from Genetically Modified (GM) Crops[J].Food and Chemical Toxicology, 2004, 42(7):1047-1088.