生态与农村环境学报 ›› 2014, Vol. 30 ›› Issue (2): 228-233.doi:

• 污染控制与修复 • 上一篇    下一篇

菹草在低浓度含铜尾水净化过程中的毒性研究

段伦超,王风贺,张帆,纪营雪,李卉,王国祥   

  1. 南京师范大学环境科学与工程系
  • 收稿日期:2013-06-28 修回日期:2014-01-24 出版日期:2014-03-25 发布日期:2014-06-07
  • 通讯作者: 王风贺 南京师范大学环境科学与工程系 E-mail:wangfenghe@njnu.edu.cn
  • 作者简介:段伦超(1988-),男,山东莱芜人,硕士生,主要研究方向为污水的污染控制。E-mail:duanlunchao@126.com
  • 基金资助:

    国家自然科学基金(41101287); 江苏省科技支撑计划(BE2012758); 江苏省建设系统科技项目(JS2011JH25)

Tolerance of Potamogeton crispus to Toxicity in Purifying Low Concentration Copper Containing Tailwater

DUAN  Lun-Chao, WANG  Feng-He, ZHANG  Fan, JI  Ying-Xue, LI  Hui, WANG  Guo-Xiang   

  1. Department of Environmental Science and Engineering,Nanjing Normal University
  • Received:2013-06-28 Revised:2014-01-24 Online:2014-03-25 Published:2014-06-07
  • Contact: WANG Feng-He Department of Environmental Science and Engineering,Nanjing Normal University E-mail:wangfenghe@njnu.edu.cn

摘要: 将菹草(Potamogeton crispus)种植于不同浓度的含游离态(CuSO4)和螯合态(EDTA-Cu)铜离子模拟尾水中,定期观测菹草在不同浓度、不同形态含铜尾水中的生长状况,并用水下饱和脉冲叶绿素荧光仪测定菹草光合作用PSII有效荧光产量(Fv/Fm)、光化学荧光淬灭系数(qP)和非光化学荧光淬灭系数(qN)等指标,以研究菹草在低浓度含铜尾水净化过程中的生态效应。结果表明,菹草对铜离子有吸附或富集作用,且对CuSO4的吸附或富集能力大于EDTA-Cu。CuSO4对菹草的毒性作用小,且对菹草生长有一定的促进作用;随着铜离子浓度(0.01、0.3、0.5和1.0 mg·L-1)的递增,CuSO4各试验组菹草叶片数增加量分别是空白组的292.5%、390.0%、155.0%和45.0%。而EDTA-Cu在试验浓度范围内对菹草的生长影响较小,毒性作用不明显。随游离态和螯合态铜离子浓度的提高,相对光合电子传递速率(RET)、Fv/FmqPqN变化明显,表明一定浓度的铜离子增强了菹草的光电子传递速率和份额,铜离子对PSⅡ反应中心的毒性作用较小,可以促进其光合作用。

关键词: 菹草, 叶绿素荧光特性参数, CuSO4, EDTA-Cu, 毒性

Abstract: In simulated tailwaters different in concentration of CuSO4 and EDTA-Cu, Potamogeton crispus was planted. Growth of the plants was monitored regularly and PSII effective fluorescence yield (Fv/Fm), photochemical quenching (qP) and non-photochemical quenching (qN) of photosynthesis of the plants were determined with a underwater saturation pulse chlorophyll fluorescence analyzer (DIVING-PAM) to explore ecological response of the plants in purifying low concentration copper containing tailwater . Results show that P. crispus could adsorb and enrich copper ions, the capacity of absorbingmore CuSO4 is higher than of EDTA-Cu. CuSO4 was less toxic to the plant and more or less conducive to its the growth of the plant. In CuSO4 treatments when CuSO4 concentration was 0.01, 0.3, 0.5 and 1.0 mg·L-1, the number of leaves per plant was 292.5%, 390.0%, 155% and 45% ,as many as that in the control, respectively. However, EDTA-Cu did not have much effect or much toxicity on growth of the plant when it was within the range of concentrations in the test. With rising concentration of CuSO4 and EDTA-Cu, RET, Fv/Fm, qP and qN varied significantly indicating that a certain concentration of copper ion can increase the transfer rate and share of photoelectrons and promote photosynthesis and is quite low in toxicity to PSII reaction center of the plants as well.

Key words: Potamogeton crispus, photosynthetic fluorescence, CuSO4, EDTA-Cu, toxicity

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