六氯苯胁迫下2种湿地植物根际土壤微生物数量与酶活性变化

doi:10.11934/j.issn.1673-4831.2018.02.011

摘要/Abstract

摘要：

为揭示人工湿地植物根际效应对氯苯类化合物生物降解的影响，选取典型湿地植物芦苇（Phragmites australis）和香蒲（Typha angustifolia），模拟室外人工湿地微宇宙系统，研究六氯苯（hexachlorobenzene，HCB）胁迫下根际土壤中微生物数量和酶活性变化。结果显示：芦苇和香蒲表现出明显的根际效应，根际土壤微生物（细菌、真菌、放线菌）数量以及土壤脱氢酶（DEH）、过氧化氢酶（CAT）和多酚氧化酶（PPO）活性均表现为根际土大于非根际土，且香蒲组土壤微生物数量和酶活性均显著高于芦苇组（P<0.05）；芦苇和香蒲组土壤中HCB含量与微生物数量和3种酶活性呈负相关，香蒲组土壤中HCB含量与DEH活性和PPO活性呈显著负相关（P<0.05）。研究表明随着芦苇和香蒲根际土壤微生物数量与酶活性的增加，2种植物的根际效应增强，进而促进湿地系统对HCB的去除。

关键词: 人工湿地, 根际效应, 微生物数量, 酶活性, 六氯苯

Abstract:

To investigate plant rhizospheric effects on biodegradation of chlorobenzene compounds in constructed wetlands, Phragmites australis and Typha angustifolia, two typical wetland plants, were selected and planted in a simulated outdoor constructed wetland mesocosm to explore changes in population of soil microbe and activity of soil enzyme in the rhizosphere of the plants under stress of hexachlorobenzene. Results show that both P. australis and T. angustifolia significantly affected soil microbe (bacteria, fungi and actinomycetes) in population and soil enzymes[dehydrogenase (DEH), catalase (CAT) and polyphenol oxidase (PPO)], in activity in the rhizosphere, which were all higher than in the non-rhizosphere soils, and the rhizosphere effects of T. angustifolia were higher than those of P. australis (P<0.05). Population of the soil microbe and activities of the soil enzymes were found negatively related to concentration of HCB in the rhizospheres of P. australis and T. angustifolia,and particularly to the activities of DEH and PPO in the rhizospheres of T. angustifolia(P<0.01). The rhizospheric effects of the two plants increased with the increase in microbial population and enzyme activity in the rhizospheres of the plants, which in turn helps remove HCB in the wetland ecosystem.

Key words: constructed wetland, rhizospheric effect, microbial population, enzyme activity, hexachlorobenzene

中图分类号:

X171.5

张翠萍, 李淑英, 王蓓, 卢国理, 朱春蓉, 周元清. 六氯苯胁迫下2种湿地植物根际土壤微生物数量与酶活性变化[J]. 生态与农村环境学报, 2018, 34(2): 177-183.

ZHANG Cui-ping, LI Shu-ying, WANG Bei, LU Guo-li, ZHU Chun-rong, ZHOU Yuan-qing. Changes in Population of Soil Microbe and Activity of Soil Enzyme in Rhizospheres of Two Wetland Plants Under Stress of Hexachlorobenzene[J]. Journal of Ecology and Rural Environment, 2018, 34(2): 177-183.

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