Cr6+暴露对红树蚬卵巢中氧化应激和生殖基因表达的影响

doi:10.19741/j.issn.1673-4831.2019.0625

摘要/Abstract

摘要： Cr⁶⁺具有较强的氧化能力，通常能够造成生物体脂质过氧化胁迫。为探究Cr⁶⁺暴露对红树蚬（Polymesoda erosa）卵巢的生殖毒性效应，共设置ρ（Cr⁶⁺）为0、0.25、1、4和8 mg·L^-1 5个浓度梯度，暴露时间为0、5、10和15 d。结果表明，Cr⁶⁺对红树蚬卵巢造成氧化胁迫，并影响卵巢雌激素受体er基因和卵黄原蛋白vtg基因表达量。与对照相比，高浓度Cr⁶⁺胁迫使红树蚬性腺指数（GSI）显著降低（P<0.05），且随着Cr⁶⁺浓度升高和试验时间增加，GSI下降程度越明显。高浓度Cr⁶⁺处理红树蚬vtg和er基因表达量显著低于低浓度处理和对照（P<0.05）。此外，通过氧化应激试验发现，经低浓度Cr⁶⁺胁迫后，红树蚬卵巢过氧化氢酶（CAT）和谷胱甘肽转移酶（GST）活性以及丙二醛（MDA）含量在10 d后与对照相比无显著差异（P>0.05）；而与对照相比，高浓度Cr⁶⁺处理CAT和GST活性被显著抑制（P<0.05），MDA含量也明显升高。Cr⁶⁺对红树蚬卵巢具有组织损伤和代谢干扰效应，并且高浓度Cr⁶⁺对红树蚬生殖存在明显毒害作用。

关键词: 红树蚬, 卵巢, Cr⁶⁺, 性腺指数, 酶活性, 生殖毒性

Abstract: Cr⁶⁺ can usually cause lipid peroxidation stress in organisms due to its strong oxidative capacity. In this study, the reproductive toxicity of Cr⁶⁺ exposure on female Polymesoda erosa was investigated. Five concentration groups of Cr⁶⁺ were set as 0, 0.25, 1, 4 and 8 mg·L^-1 and the exposure time of each concentration group was set as 0, 5, 10 and 15 d. The results indicate that the exposure to Cr⁶⁺ could cause oxidative stress on the ovaries of Polymesoda erosa and change the expression of er (estrogen receptor gene) and vtg (vitellogenin gene). Compared with control group, the high concentration of Cr⁶⁺ treatment groups could significantly reduce the gonadosomatic index (GSI) of Polymesoda erosa (P<0.05). Moreover, the GSI varied significantly with the increase of Cr⁶⁺ concentration as time goes by. The expression of vtg and er in high concentration treatment group was significantly lower than that in low concentration treatment group as well as control group (P<0.05). In addition, it was found that the activities of CAT, GST and MDA content in ovaries would get back to normal level (P>0.05) after exposing to low concentration of Cr⁶⁺. On the other hand, high concentration of Cr⁶⁺ exposure would eventually inhibit the activities of both enzymes (P<0.05) and increase the content of MDA significantly. The results manifest that Cr⁶⁺ has tissue damage and metabolic disturbance effects on the ovaries of Polymesoda erosa and high concentration of Cr⁶⁺ would cause obvious toxic effects on the reproduction of Polymesoda erosa.

Key words: Polymesoda erosa, ovary, Cr⁶⁺, gonad index, enzyme activity, reproductive toxicity

中图分类号:

Q95-3

程遂, 逯峰, 黄勃, 郭云鹏. Cr⁶⁺暴露对红树蚬卵巢中氧化应激和生殖基因表达的影响[J]. 生态与农村环境学报, 2020, 36(3): 399-405.

CHENG Sui, LU Feng, HUANG Bo, GUO Yun-peng. Effects of Cr⁶⁺ Exposure on Oxidative Stress and Reproductive Gene Expression in the Ovaries of Polymesoda erosa[J]. Journal of Ecology and Rural Environment, 2020, 36(3): 399-405.

参考文献 27

[1]	蔡英亚,黄翔鹄,吴洞科.红树蚬的生态观察[J].热带海洋,1995,14(1):94-98.[CAI Ying-ya,HUANG Xiang-hu,WU Dong-ke.Studies on the Ecology of Polymesoda erosa (Solander)[J].Tropic Oceanology,1995,14(1):94-98.]
[2]	张俊杰,潘红平,姜恒,等.红树蚬壳体尺性状对活体重的影响研究[J].安徽农业科学,2012,40(30):14730,14736.[ZHANG Jun-jie,PAN Hong-ping,JIANG Heng,et al.Study on the Effects of Shell Size Traits on the Live Weight of Polymesoda erosa[J].Journal of Anhui Agricultural Sciences,2012,40(30):14730,14736.]
[3]	张俊杰.红树蚬的生物学研究[D].南宁:广西大学,2013.[ZHANG Jun-jie.Biological Study of the Mangrove Clam Polymesoda (Geloina) erosa [D].Nanning:Guangxi University,2013]
[4]	周浩郎,张俊杰,邢永泽,等.广西红树蚬的分布特征及影响因素分析[J].广西科学,2014,21(2):147-152.[ZHOU Hao-lang,ZHANG Jun-jie,XING Yong-ze,et al.Characteristics of Distribution and the Influential Factors of Mangrove Clam, Polymesoda erosa (Solander 1786),in Guangxi[J].Guangxi Sciences,2014,21(2):147-152.]
[5]	AMOOZADEH E,MALEK M,RASHIDINEJAD R,et al.Marine Organisms as Heavy Metal Bioindicators in the Persian Gulf and the Gulf of Oman[J].Environmental Science and Pollution Research,2014,21(3):2386-2395.
[6]	张庆鸿.锌与双酚A胁迫下福建牡蛎性腺差异表达蛋白的研究[D].厦门:厦门大学,2014.[ZHANG Qing-hong.Different Proteomics Profiling of Crassostrea angulata Gonad Exposed to Zn and Bisphenol-A[D].Xiamen:Xiamen University,2014.]
[7]	张林宝,孙伟,张喆,等.三唑磷农药对翡翠贻贝性激素水平和性腺损伤效应的研究[J].南方水产科学,2018,14(2):60-65.[ZHANG Lin-bao,SUN Wei,ZHANG Zhe,et al.Influence of Triazophos on Sex Hormones Levels and Damage Effect of Perna viridis Gonad[J].South China Fisheries Science,2018,14(2):60-65.]
[8]	赖廷和,何斌源,范航清,等.重金属Cd胁迫对红树蚬的抗氧化酶、消化酶活性和MDA含量的影响[J].生态学报,2011,31(11):3044-3053.[LAI Ting-he,HE Bin-yuan,FAN Hang-qing,et al.Effects of Cadmium Stress on the Activities of Antioxidant Enzymes,Digestive Enzymes and the Membrane Lipid Peroxidation of the Mangrove Mud Clam Geloina coaxans (Gmelin)[J].Acta Ecologica Sinica,2011,31(11):3044-3053.]
[9]	陈红星,吴星,毕然,等.水环境中Cr(Ⅵ)对鱼类毒性机理研究进展[J].应用生态学报,2015,26(10):3226-3234.[CHEN Hong-xing,WU Xing,BI Ran,et al.Mechanisms of Cr(Ⅵ) Toxicity to Fish in Aquatic Environment:A Review[J].Chinese Journal of Applied Ecology,2015,26(10):3226-3234.]
[10]	LUSHCHAK V I.Environmentally Induced Oxidative Stress in Aquatic Animals[J].Aquatic Toxicology,2011,101(1):13-30.
[11]	冷春玲,李春茂,迟庆宏,等.贻贝雌性生殖系统的组织学研究[J].水产科学,1999,18(3):26-29.DOI:10.16378/j.cnki.1003-1111.1999.03.007.[LENG Chun-ling,LI Chun-mao,CHI Qing-hong,et al.Histology of the Femalereproductive System in Mussel Mytilus edulis (Lamellibranchia,Mytiloida)[J].Fisheries Science,1999,18(3):26-29.DOI:10.16378/j.cnki.1003-1111.1999.03.007.]
[12]	OSADA M,TAKAMURA T,SATO H,et al.Vitellogenin Synthesis in the Ovary of Scallop,Patinopecten yessoensis:Control by Estradiol-17β and the Central Nervous System[J].Journal of Experimental Zoology Part A:Comparative Experimental Biology,2003,299A(2):172-179.
[13]	GRIGORAKIS K,ALEXIS M N.Effects of Fasting on the Meat Quality and Fat Deposition of Commercial-size Farmed Gilthead Sea Bream (Sparus aurata,L.) Fed Different Dietary Regimes[J].Aquaculture Nutrition,2005,11(5):341-344.
[14]	WANG G S,ZHANG C K,HUANG B.Transcriptome Analysis and Histopathological Observations of Geloina erosa Gills Upon Cr(Ⅵ) Exposure[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2020,231:108706.
[15]	车志群,孙仁杰,刘文爱,等.六溴环十二烷胁迫下红树蚬荧光定量PCR内参基因稳定性分析[J].广西科学院学报,2018,34(3):242-249.[CHE Zhi-qun,SUN Ren-jie,LIU Wen-ai,et al.The Expression Stability Analysis of Reference Genes for qRT-PCR in Polymesoda erosa Under Hexabromocyclododecane Stress[J].Journal of Guangxi Academy of Sciences,2018,34(3):242-249.]
[16]	陈海刚,林钦,蔡文贵,等.3种常见海洋贝类对重金属Hg、Pb和Cd的积累与释放特征比较[J].农业环境科学学报,2008,27(3):1163-1167.[CHEN Hai-gang,LIN Qin,CAI Wen-gui,et al.Comparisons on the Accumulation and Elimination Characteristic of Hg,Pb and Cd in Three Kinds of Marine Bivalve Molluscs[J].Journal of Agro-Environment Science,2008,27(3):1163-1167.]
[17]	程家丽,张贤辉,卓勤,等.我国海洋食用贝类重金属污染特征及其健康风险[J].中国食品卫生杂志,2016,28(2):175-181.[CHENG Jia-li,ZHANG Xian-hui,ZHUO Qin,et al.Accumulation and Health Risks of Heavy Metals in Edible Marine Shellfishes From China[J].Chinese Journal of Food Hygiene,2016,28(2):175-181.]
[18]	孙莉娜,黄玉英.厦门沿海贝类中重金属的含量及污染状况评价[J].东华理工大学学报(自然科学版),2018,41(2):181-184.[SUN Li-na,HUANG Yu-ying.The Assessment of Heavy Metals Content and Pollution of Shellfish Along the Coast of Xiamen[J].Journal of East China University of Technology(Natural Science),2018,41(2):181-184.]
[19]	杨丽华,査金苗,王子健.乙烯菌核利对稀有鮈鲫性腺组织及相关基因转录水平的影响[J].生态毒理学报,2014,9(2):245-252.[YANG Li-hua,ZHA Jin-miao,WANG Zi-jian.Vinclozolin Affected the Gonad of Rare Minnow (Gobiocypris rarus) at Histopathological and Transcriptional Levels[J].Asian Journal of Ecotoxicology,2014,9(2):245-252.]
[20]	穆希岩,黄瑛,沈公铭,等.苯醚甲环唑对斑马鱼抗氧化酶的影响[J].中国环境科学,2016,36(4):1242-1249.[MU Xi-yan,HUANG Ying,SHEN Gong-ming,et al.Impact of Difenoconazole on the Antioxidases of Zebrafish(Danio rerio)[J].China Environmental Science,2016,36(4):1242-1249.]
[21]	董学兴,吕林兰,吕富,等.17β-雌二醇对双齿围沙蚕幼虫SOD、CAT和GST活性的影响[J].南方水产科学,2014(6):83-87.[DONG Xue-xing,LÜ Lin-lan,LÜ Fu,et al.Effect of 17β-estradiol on Activities of SOD,CAT and GST in Larvae of Perinereis aibuhitensis[J].South China Fisheries Science,2014(6):83-87.]
[22]	梁晓敏,曾凤,张群芳,等.汞镉联合暴露对斑马鱼性腺组织学和抗氧化酶基因表达的影响[J].重庆师范大学学报(自然科学版),2018,35(3):58-63,2.[LIANG Xiao-min,ZENG Feng,ZHANG Qun-fang,et al.Effects of Combined Exposure to Mercury and Cadmium on Histology and the Expression of Antioxidative Enzyme Genes in Gonads of Zebrafish[J].Journal of Chongqing Normal University(Natural Science),2018,35(3):58-63,2.]
[23]	BEBIANNO M J,COMPANY R,SERAFIM A,et al.Antioxidant Systems and Lipid Peroxidation in Bathymodiolus azoricus From Mid-atlantic Ridge Hydrothermal Vent Fields[J].Aquatic Toxicology,2005,75(4):354-373.
[24]	VELMA V,TCHOUNWOU P B.Oxidative Stress and DNA Damage Induced by Chromium in Liver and Kidney of Goldfish, Carassius auratus[J].Biomarker Insights,2013,8:BMI.S11456.
[25]	ZHANG Q F,LI Y W,LIU Z H,et al.Reproductive Toxicity of Inorganic Mercury Exposure in Adult Zebrafish:Histological Damage,Oxidative Stress,and Alterations of Sex Hormone and Gene Expression in the Hypothalamic-pituitary-gonadal Axis[J].Aquatic Toxicology,2016,177:417-424.
[26]	MARIN M G,MATOZZO V.Vitellogenin Induction as a Biomarker of Exposure to Estrogenic Compounds in Aquatic Environments[J].Marine Pollution Bulletin,2004,48(9/10):835-839.
[27]	MATSUMOTO T,OSADA M,OSAWA Y,et al.Gonadal Estrogen Profile and Immunohistochemical Localization of Steroidogenic Enzymes in the Oyster and Scallop During Sexual Maturation[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1997,118(4):811-817.

Cr⁶⁺暴露对红树蚬卵巢中氧化应激和生殖基因表达的影响

Effects of Cr⁶⁺ Exposure on Oxidative Stress and Reproductive Gene Expression in the Ovaries of Polymesoda erosa

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