青霉素对土壤微生物群落结构的影响

doi:10.11934/j.issn.1673-4831.2016.02.021

Abstract

Abstract:

Effect of penicillin, varying in concentration, on metabolic activity and structural diversity of soil microbial community was analyzed with the Biolog-ECO technology, and changes in the soil microbial community structure were with the DGGE (denaturing gradient gel electrophoresis) technology, so as to explore eco-toxicological effect of penicillin on soil microbial community. PCA based on the BiologEcoplates^TM shows that penicillin high in concentration (400-800 mg·kg^-1) may induce rapid enrichment of microbial groups that use phenolic compounds as carbon source in the soil microbial community. PCR-DGGE analysis reveals that in the soil treated with a high dose(800 mg·kg^-1) of penicillin, Shannon-Wiener diversity index was significantly lowered in the first days and gradual recovered 7 days later with the penicillin in the soil degrading.

Key words: penicillin, microbial diversity, BiologEcoplates^TM, PCR-DGGE

CLC Number:

LIU Yan, ZHANG Zhen-hua, BAO Wang-bo, YU Ran, WANG Chang-yong. Effects of Penicillin on Soil Microbial Community Structure[J]. Journal of Ecology and Rural Environment, 2016, 32(2): 309-314.

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Effects of Penicillin on Soil Microbial Community Structure

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