小麦秸秆不同还田方式下土壤微生物碳代谢多样性特征

doi:10.11934/j.issn.1673-4831.2017.10.007

Abstract

Abstract:

Straw incorporation is an important measure to improve soil carbon sequestration potential in paddy fields. The research to characterize soil microbial biomass carbon metabolism as affected by straw incorporation method or pattern means significantly to utilization of crop straw and soil carbon sequestration. To explore effects of straw incorporations different in pattern on soil microbiological characteristics, four treatments were designed, i. e. incorporation of straw used as padding of a fermentation bed (SP), incorporation of carbonized straw or biochar as supplement to chemical fertilization (BR), incorporation of straw plus composted pig dung (OF) and chemical fertilization (CF), in addition to CK (no fertilization), for the field experiment of the study. All the treatments were equivalently the same in N application rate. The biology-ECO technology was used to reveal diversity of soil microbial community structure, and PCA performed to characterize variation of the soil microbial community structure. Results show that straw incorporation pattern was an important factor affecting soil microbial carbon metabolism in characteristic. Treatment OF was obviously higher than all the other ones in effect on soil microbial carbon metabolism capacity, microbial diversity and saccharides, amino acid, polymers and amine utilization rates, and Treatment SP was much lower than Treatments OF and BR in effect on soil microbe. Straw incorporation pattern could also significantly affect soil microbial carbon utilization in the top soil layer (0-5 cm) and sub-surface soil layer (>5-10 cm), but not so much in the subsoil layer (>10-20 cm). It also affected soil microbial diversity. Compared with Treatments CF and BR, Treatments OF and SP improved soil microbial diversity. It is, therefore, held that OF and BR may significantly improve soil microbial carbon metabolism diversity and soil microbial carbon utilization within a short period of time.

Key words: straw-incorporation, biochar, fermentation bed padding, Biolog-ECO

CLC Number:

Q143
S154.36

ZHOU Yun-lai, ZHANG Zhen-hua, FAN Ru-qin, QIAN Xiao-qing, LU Xin, LIU Li-zhu. Carbon Metabolism Diversity Characteristics of Soil Microbe Affected by Wheat Straw Incorporation Pattern[J]. Journal of Ecology and Rural Environment, 2017, 33(10): 913-920.

References

[1] 韦茂贵,王晓玉,谢光辉.中国各省大田作物田间秸秆资源量及其时间分布[J].中国农业大学学报,2012,17(6):32-44.
[2] 曹国良,张小曳,王亚强,等.中国区域农田秸秆露天焚烧排放量的估算[J].科学通报,2007,52(15):1826-1831.
[3] 张斯梅,杨四军,石祖梁,等.江苏省稻麦秸秆收集利用现状分析及对策[J].生态与农村环境学报,2014,30(6):706-710.
[4] 李玮,乔玉强,陈欢,等.秸秆还田配施氮肥对砂姜黑土有机质组分与碳库管理指数的影响[J].生态与农村环境学报,2014,30(4):475-480.
[5] MACDONALD L M,FARRELL M,VAN ZWIETEN L,et al.Plant Growth Responses to Biochar Addition:An Australian Soils Perspective[J].Biology and Fertility of Soils,2014,50(7):1035-1045.
[6] 陆扬,吴淑杭,周德平,等.发酵床养猪垫料的养分转化与植物毒性研究[J].农业环境科学学报,2011,30(7):1409-1412.
[7] 潘根兴,张阿凤,邹建文,等.农业废弃物生物黑炭转化还田作为低碳农业途径的探讨[J].生态与农村环境学报,2010,26(4):394-400.
[8] 饶霜,卢阳,黄飞,等.生物炭对土壤微生物的影响研究进展[J].生态与农村环境学报,2016,32(1):53-59.
[9] DEGENS B P,SCHIPPER L A,SPARLING G P,et al.Decreases in Organic C Reserves in Soils Can Reduce the Catabolic Diversity of Soil Microbial Communities[J].Soil Biology and Biochemistry,2000,32(2):189-196.
[10] 罗希茜,郝晓晖,陈涛,等.长期不同施肥对稻田土壤微生物群落功能多样性的影响[J].生态学报,2009,29(2):740-748.
[11] 贺纪正.面向未来的土壤微生物生态学研究[C]//面向未来的土壤科学.成都:电子科技大学出版社,2012.
[12] 靳蓉,苏静,刘红宇,等.应用BIOLOG ECO微培养技术研究样品中微生物的碳源代谢特征[C]//第三届全国微生物资源学术暨国家微生物资源平台运行服务研讨会会议论文摘要集.[出版地不详]:[出版者不详],2011.[s.l.]:[s.n.],2011.]
[13] 李晓莎,武宁,刘玲,等.不同秸秆还田和耕作方式对夏玉米农田土壤呼吸及微生物活性的影响[J].应用生态学报,2015,26(6):1765-1771.
[14] BELYAEVA O N,HAYNES R J.Comparison of the Effects of Conventional Organic Amendments and Biochar on the Chemical,Physical and Microbial Properties of Coal Fly Ash as a Plant Growth Medium[J].Environmental Earth Sciences,2012,66(7):1987-1997.
[15] 邹春娇,齐明芳,马建,等.Biolog-ECO解析黄瓜连作营养基质中微生物群落结构多样性特征[J].中国农业科学,2016,49(5):942-951.
[16] BRADIEY R L,SHIPLEY B,BEAULIEU C.Refining Numerical Approaches for Analyzing Soil Microbial Community Catabolic Profiles Based on Carbon Source Utilization Patterns[J]. Soil Biology and Biochemistry,2006,38(3):629-632.
[17] CHOI K H,DOBBS F C.Comparison of Two Kinds of Biolog Microplates (GN and ECO) in Their Ability to Distinguish Among Aquatic Microbial Communities[J].Journal of Microbiological Methods,1999,36(3):203-213.
[18] MAGURRAN A E.Ecological Diversity and Its Measurement[M].New Jersey:Princeton University Press,1988:43-47.
[19] 王秀国.杀菌剂多菌灵高频投入对土壤微生物群落的影响及其生物修复[D].杭州:浙江大学,2009.
[20] 王军.莠去津对土壤微生物群落结构及分子多样性的影响[D].泰安:山东农业大学,2012.
[21] JACKSON L E,CALDERON F J,STEENWERTH K L,et al.Responses of Soil Microbial Processes and Community Structure to Tillage Events and Implications for Soil Quality[J].Geoderma,2003,114(3/4):305-317.
[22] 孔维栋,刘可星,廖宗文.有机物料种类及腐熟水平对土壤微生物群落的影响[J].应用生态学报,2004,15(3):487-492.
[23] 郑丽萍,龙涛,林玉锁,等.Biolog-ECO解析有机氯农药污染场地土壤微生物群落功能多样性特征[J].应用与环境生物学报,2013,19(5):759-765.
[24] 郭梨锦,曹凑贵,张枝盛,等.耕作方式和秸秆还田对稻田表层土壤微生物群落的短期影响[J].农业环境科学学报,2013,32(8):1577-1584.
[25] 顾美英,唐光木,葛春辉,等.不同秸秆还田方式对和田风沙土土壤微生物多样性的影响[J].中国生态农业学报,2016(4):489-498.
[26] SUTTON R,SPOSITO G.Molecular Structure in Soil Humic Substances:The New View[J].Environmental Science & Technology,2005,39(23):9009-9015.
[27] 唐景春,孙青,王如刚,等.堆肥过程中腐殖酸的生成演化及应用研究进展[J].环境污染与防治,2010(5):73-77.
[28] ABUJABHAH I S,BOUND S A,DOYLE R,et al.Effects of Biochar and Compost Amendments on Soil Physico-Chemical Properties and the Total Community Within a Temperate Agricultural Soil[J].Applied Soil Ecology,2016,98:243-253.
[29] WANG J Y,XIONG Z Q,KUZYAKOY Y.Biochar Stability in Soil:Meta-Analysis of Decomposition and Priming Effects[J].Bioenergy,2015,8(3):512-523.
[30] 孙悦,徐兴良,YAKOV K.根际激发效应的发生机制及其生态重要性[J].植物生态学报,2014,38(1):62-75.
[31] 吕美蓉,李忠佩,刘明,等.长期有机无机肥配合施用土壤中添加不同肥料养分后土壤微生物短期变化[J].生态与农村环境学报,2011,27(4):69-73.
[32] GUO L J,LIN S,LIU T Q,et al.Effects of Conservation Tillage on Topsoil Microbial Metabolic Characteristics and Organic Carbon Within Aggregates Under a Rice (Oryza sativa L.):Wheat (Triticum aestivum L.) Cropping System in Central China[J].PLoS One,2016,11(1):e0146145.
[33] 李晨华,张彩霞,唐立松,等.长期施肥土壤微生物群落的剖面变化及其与土壤性质的关系[J].微生物学报,2014,54(3):319-329.

Carbon Metabolism Diversity Characteristics of Soil Microbe Affected by Wheat Straw Incorporation Pattern

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