生态与农村环境学报 ›› 2015, Vol. 31 ›› Issue (5): 774-778.doi: 10.11934/j.issn.1673-4831.2015.05.025

• 污染控制与修复 • 上一篇    下一篇

耐砷真菌分离鉴定及对土壤生物有效砷的影响

姜朵朵,孙璐,卜元卿,张梅华,蒋继宏,高吉喜   

  1. 中节能六合天融环保科技有限公司
  • 收稿日期:2015-02-02 修回日期:2015-07-01 出版日期:2015-09-25 发布日期:2015-09-30
  • 通讯作者: 高吉喜 E-mail:gjx@nies.org;
  • 作者简介:姜朵朵(1987-),女,湖南邵阳人,助理工程师,硕士,主要研究方向为土壤重金属微生物治理。E-mail:jiangduoduo123456@163.com
  • 基金资助:

    国家自然科学基金(31370646)

Isolation,Identification and Effect of Three Arsenic-Resistant Strains on Bioavailable Arsenic in Soil.

JIANG Duo-duo,SUN Lu,BU Yuan-qing,ZHANG Mei-hua,JIANG Ji-hong,GAO Ji-xi   

  1. China Energy Corporation and Environmental Protection Group (CECEP) Liuhe Talroad Environmental Technology Co. Ltd.
  • Received:2015-02-02 Revised:2015-07-01 Online:2015-09-25 Published:2015-09-30

摘要:

从砷污染土壤中分离出 3 株对砷具有耐受性的真菌菌株,考察了菌株对砷的耐受能力及菌体处理污染土 壤时间、菌体投加量对土壤生物砷有效性的影响。经鉴定,3 种菌株分别为球毛壳霉(Chaetomium globosum)、细极 链格孢(Alternaria tenuissima)和木糖发酵酵母(Scheffersomyces stipitis)。结果表明:3 种菌株对 ρ 为 5~100 mg•L-1的砷具有良好的耐受性,且耐受性从大到小依次为木糖发酵酵母、细极链格孢和球毛壳霉;ρ 为5~10 mg•L-1的砷 对木糖发酵酵母的生长有一定的促进作用,30 d 内土壤生物有效态砷含量与对照相比分别提高 137.2、77.9 和 36.5 倍;不同浓度菌体对于土壤砷具有不同的作用效果,土壤有效态砷含量随球毛壳霉、细极链格孢和木糖发酵酵母投加量的增加而增加,且两者呈正相关。

关键词:  耐砷性, 真菌, 生物有效态

Abstract:

Three arsenic-resistant strains of fungi were isolated and identified as Chaetomiumglobosum, Alternaria tenuissima and Scheffersomyces stipitis from arsenic contaminated soils, and tested for tolerance of the strains to As and effects of the treatment of As-contaminated soils with the strains on soil As bio-availability relative to duration of the exposure to and inoculation rate of the fungi. Results show that the three strains were quite good in tolerance to As5+ in the range of 5-100 mg•L-1 As5+ and displayed an order of Scheffersomyces stipitis > Alternaria tenuissima > Chaetomium globosum. When As concentration was in the range of 5-10 mg•L-1, Scheffersomyces stipitis was somewhat promoted in growth. Within the first 30 days of the inoculation of the three strains, soil bio?available As increased by 137.2, 77.9 and 36.5 times, respectively, as compared with CK. Their effects on soil As varied with their inoculation rate, displaying a rising trend with the rate and a positive relationship.

Key words: arsenic tolerance, fungi, bio-available form