玉米接种丛枝菌根真菌后对土壤铅污染的耐受效应

doi:10.19741/j.issn.1673-4831.2022.0314

生态与农村环境学报 ›› 2023, Vol. 39 ›› Issue (10): 1316-1322.doi: 10.19741/j.issn.1673-4831.2022.0314

玉米接种丛枝菌根真菌后对土壤铅污染的耐受效应

杨怡森^1,2, 孙晨瑜^1,2, 马俊卿^1,2, 覃圣峰^1,2, 周勋波^1,2,3, 黄京华^1,2,3

1. 广西大学农学院, 广西南宁 530004;
2. 广西高校作物栽培学与耕作学重点实验室, 广西南宁 530004;
3. 广西大学植物科学国家级实验教学示范中心, 广西南宁 530004

收稿日期:2022-04-12 出版日期:2023-10-25 发布日期:2023-10-23
通讯作者: 黄京华,E-mail:hjhscau@163.com E-mail:hjhscau@163.com
作者简介:杨怡森(1996-),男,河南洛阳人,硕士,研究方向为作物生态与安全生产。E-mail:18438616368@163.com
基金资助:
国家自然科学基金(31760137)

Effect of Arbuscular Mycorrhizal Fungi (AMF) on Maize Tolerance to Lead (Pb) Contaminated Soil

YANG Yi-sen^1,2, SUN Chen-yu^1,2, MA Jun-qing^1,2, QIN Sheng-feng^1,2, ZHOU Xun-bo^1,2,3, HUANG Jing-hua^1,2,3

1. College of Agriculture, Guangxi University, Nanning 530004, China;
2. Key Laboratory of Colleges and Universities of Guangxi for Crop Cultivation and Farming System, Nanning 530004, China;
3. State Demonstration Center for Experimental Plant Science Education, Guangxi University, Nanning 530004, China

Received:2022-04-12 Online:2023-10-25 Published:2023-10-23

摘要/Abstract

摘要： 重金属污染条件下植物的农艺性状及生理生化指标均会受到不同程度的影响。丛枝菌根因其独有的性质,在植物应对非生物胁迫过程中发挥着重要作用。为研究玉米与丛枝菌根真菌(arbuscular mycorrhizal fungi, AMF)共生后对土壤铅的耐受效应,采用盆栽方法种植玉米,用外源施加Pb(NO₃)₂老化15 d后的土壤模拟农田铅污染环境,探讨不同铅含量(0、170、570、970 mg·kg^-1)下接种不同AMF〔摩西管柄囊霉(Funneliformis mosseae)Fm;幼套近明球囊霉(Claroideoglomus etunicatum,Ce)、地表球囊霉(Glomus versiforme,Gv)〕和不接种AMF(NM)对玉米生长的影响,分析了接种AMF后玉米农艺性状、抗氧化能力及铅含量的变化。结果表明:铅污染下玉米菌根侵染率相比无铅污染处理高15.0%~36.0%;接菌玉米的株高增加11.8%~26.8%,叶绿素含量提高3.9%~7.5%,鲜重增加50%;玉米茎叶铅含量显著降低,根系铅含量显著增加,铅的转移系数降低(P＜0.05)。抗性分析表明,菌根共生使玉米叶片SOD活性受抑制,根系SOD活性显著提高,POD活性在叶片和根系中均提高,根系CAT活性在铅浓度较高时增加。菌根共生使玉米根系脯氨酸含量降低,叶片和根系可溶性蛋白含量显著增加(P＜0.05)。综上,铅污染条件下接种AMF可促进玉米生长,提高玉米体内抗氧化酶活性,使可溶性蛋白含量显著提升,增加铅在根系中的积累,减少向地上部转移,从而减轻铅对地上部光合作用等生理过程的抑制,提高玉米对铅的耐受性。

关键词: 玉米, 铅污染, 丛枝菌根真菌, 抗氧化酶, 耐性

Abstract: The agronomic characters and physio-chemical indexes of plants under heavy metal pollution will be affected in varying degrees. Arbuscular mycorrhizal fungi (AMF) play an important role in plant response to abiotic stress due to their unique properties. In order to understand the tolerance effect of maize to Pb after symbiosis with arbuscular mycorrhizal fungi (AMF), the environment led by Pb pollution in the maize farmland was simulated using exogenous Pb(NO₃)₂ aging soil for 15 days. The effects of AMF inoculation (Funneliformis mosseae, Fm; Claroideoglomus etunicatum, Ce; Glomus versiforme, Gv) and no AMF inoculation (NM) on the growth of maize at different Pb concentrations (0, 170, 570, 970 mg·kg^-1) were investigated in pot experiment, and the changes of agronomic traits, antioxidant capacity and Pb content of maize inoculated with AMF were analyzed. The results show that the colonization rate of mycorrhizal under Pb contamination was 15.0%-36.0% higher than that without Pb contamination. The height and chlorophyll content of colonized maize were increased by 11.8%-26.8% and 3.9%-7.5%, respectively, and the fresh weight increased by 50%. The translocation factor decreased, meanwhile the Pb content in stem and leaf of maize decreased significantly, but for root it increased significantly (P＜0.05). Resistance analysis indicate that the formation of mycorrhiza inhibited the activity of SOD in maize leaves, but significantly increased the activity of SOD in maize roots, while the activity of POD in the leaves and roots increased. When the concentration of Pb was high, the activity of CAT in roots increased significantly (P＜0.05). The formation of mycorrhiza resulted in the decrease of proline content in maize roots and the increase of soluble protein content in maize leaves and roots (P＜0.05). Under the condition of Pb pollution, AMF inoculation increased the antioxidant capacity of maize, increased the content of soluble protein in maize leaves and roots, and improved the water retention capacity, so as to enhance the resistance of maize to Pb pollution and ensure the normal growth of maize in Pb-contaminated soil. This study provides a theoretical basis for the remediation of heavy metal contaminated soil in farmland and the safe production of crops.

Key words: maize, lead pollution, arbuscular mycorrhizal fungi, antioxidant enzyme, tolerance

中图分类号:

S513

杨怡森, 孙晨瑜, 马俊卿, 覃圣峰, 周勋波, 黄京华. 玉米接种丛枝菌根真菌后对土壤铅污染的耐受效应[J]. 生态与农村环境学报, 2023, 39(10): 1316-1322.

YANG Yi-sen, SUN Chen-yu, MA Jun-qing, QIN Sheng-feng, ZHOU Xun-bo, HUANG Jing-hua. Effect of Arbuscular Mycorrhizal Fungi (AMF) on Maize Tolerance to Lead (Pb) Contaminated Soil[J]. Journal of Ecology and Rural Environment, 2023, 39(10): 1316-1322.

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玉米接种丛枝菌根真菌后对土壤铅污染的耐受效应

Effect of Arbuscular Mycorrhizal Fungi (AMF) on Maize Tolerance to Lead (Pb) Contaminated Soil

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