生态与农村环境学报 ›› 2012, Vol. 28 ›› Issue (5): 526-531.doi:

• 污染控制与修复 • 上一篇    下一篇

河流底泥施用对土壤微生物群落功能多样性和小麦生长的影响

华建峰,杨亦茹,徐建华,王兴祥,殷云龙   

  1. 江苏省中国科学院植物研究所
  • 收稿日期:2012-03-10 修回日期:2012-05-08 出版日期:2012-09-25 发布日期:2012-10-08
  • 通讯作者: 殷云龙 江苏省中国科学院植物研究所 E-mail:yinyl066@sina.com
  • 作者简介:华建峰(1980-),男,江苏无锡人,助理研究员,博士,主要研究方向为植物资源与环境。E-mail:jfhua2009@gmail.com
  • 基金资助:

    国家自然科学基金青年科学基金(41101232);江苏省自然科学基金(BK2011688)

Effects of River Sediment Application on Functional Diversity of Soil Microbial Community and Growth of Winter Wheat

HUA  Jian-Feng, YANG  Yi-Ru, XU  Jian-Hua, WANG  Xing-Xiang, YIN  Yun-Long   

  1. Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences
  • Received:2012-03-10 Revised:2012-05-08 Online:2012-09-25 Published:2012-10-08
  • Contact: YIN Yun-Long Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences E-mail:yinyl066@sina.com

摘要: 设置0(对照)、20、60和100 g·kg-1 3个底泥施用量水平,研究施用底泥对冬小麦籽粒Cd含量以及土壤微生物群落功能多样性的影响。结果表明,60和100 g·kg-1底泥施用处理小麦穗质量和千粒重显著高于对照(P<0.05)。各处理间小麦籽粒Cd含量无显著差异。与对照相比,施用底泥并没有显著改变土壤中可培养细菌、真菌 和放线菌的数量。整个培养过程中土壤微生物平均颜色变化率(IAWCD)以及96 h时微生物多样性指数(Shannon 指数、Simpson指数、McIntosh指数)在对照与底泥施用处理间无显著差异。主成分分析(PCA)表明,仅有占总变量 方差14.22%的PC3能识别出对照土壤微生物群落碳源利用谱与底泥施用处理间的差异,而PC1(21.76%)、PC2 (16.32%)和PC4(11.62%)则无法将对照与底泥施用处理区分开来。可见,该试验条件下合理的底泥施用量并不会对小麦籽粒Cd含量、微生物功能多样性和土壤质量产生显著影响。

关键词: 底泥农用, 土壤微生物, 功能多样性, 籽粒, Cd, Biolog分析

Abstract: Soil microbial organisms are an important components of farmland ecosystems, and soil microbial community structure and activity reflects quality of the soil to some extent. A pot experiment was conducted to investigate effects of application of river sediment on population and functional diversity of soil microbe and Cd content of winter wheat grain under four different sediment application levels, 0 (control), 20, 60 and 100 g•kg-1. Results show that spike quality and 1 000-grain weight of wheat were much higher (P < 0.05) in Treatments 60 and 100 g•kg-1 than in control and no significant difference was found in grain Cd content between the four river sediment application rates. Compared with control, application of river sediment had no significant influence on populations of bacteria, fungi and actinomyce, and no significant influence on average well color development (IAWCD), Shannon index (H'), Simpson index (D) and McIntosh index (U) of microbe at 96 h, either. Principal component analysis (PCA) demonstrated that only by PC3 which explains 14.22% of the total variable variance, can the differences between control and the treatments be distinquished in carbon utilization of soil microbial community, while PC1, PC2 and PC4 which explains 21.76%, 16.32% and 11.62%, respectively, of the total variable variance are useless in this aspect. All of the above imply that in this study river sediment application rate has no significant effects on Cd content of winter wheat grain, functional diversity of soil microbe and soil quality.

Key words: river sediment application, soil microbe, function diversity, winter wheat grain, Cd, Biolog analysis

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