暗灰链霉菌CGMCC 13662降解苯甲腈和苯甲酸的研究

doi:10.19741/j.issn.1673-4831.2018.0854

生态与农村环境学报 ›› 2020, Vol. 36 ›› Issue (2): 257-264.doi: 10.19741/j.issn.1673-4831.2018.0854

暗灰链霉菌CGMCC 13662降解苯甲腈和苯甲酸的研究

郭铃¹, 方文婉¹, 葛峰², 戴亦军¹

1. 南京师范大学生命科学学院/江苏省微生物与功能基因组学重点实验室/江苏省微生物资源产业化工程技术研究中心, 江苏南京 210023;
2. 生态环境部南京环境科学研究所, 江苏南京 210042

收稿日期:2019-04-21 发布日期:2020-03-03
通讯作者: 葛峰,E-mail:gefeng@nies.org E-mail:gefeng@nies.org
作者简介:郭铃(1994-),女,湖南湘潭人,博士生,主要从事微生物资源利用与环境修复研究。E-mail:lingguosince1994@163.com
基金资助:
国家自然科学基金（31570104）

Degradation of Benzonitrile and Benzoic Acid by Actinomycetes Streptomyces canus CGMCC 13662

GUO Ling¹, FANG Wen-wan¹, GE Feng², DAI Yi-jun¹

1. College of Life Science/Jiangsu Key Laboratory for Microbes and Functional Genomics/Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Nanjing Normal University, Nanjing 210023, China;
2. Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China

Received:2019-04-21 Published:2020-03-03

摘要/Abstract

摘要： 筛选到一株能降解腈类化合物的暗灰链霉菌（Streptomyces canus）CGMCC 13662，该菌的生长细胞能在120 h内完全降解0.5 g·L^-1苯甲腈，而其静息细胞仅需24 h就能完全降解苯甲腈。生长细胞只能将苯甲腈代谢为苯甲酰胺，而静息细胞还能继续将苯甲酰胺代谢为苯甲酸并进一步降解。暗灰链霉菌CGMCC 13662能同时降解苯甲酸代谢的中间产物儿茶酚和原儿茶酸，表明该菌株代谢苯甲酸主要通过儿茶酚和原儿茶酸途径。CoCl₂诱导的菌体在苯甲腈代谢的前8 h内产生苯甲酸的量比无CoCl₂诱导时低6倍，CoCl₂可改变苯甲腈代谢流。苯甲腈的降解速率在酸性条件下降低，而苯甲酰胺的降解对pH值变化敏感，仅在pH值为7.5时达到最高。添加苹果酸盐后苯甲腈的降解半衰期由5.4减少到3.8 h，是转化苯甲腈的最适共代谢基质。葡萄糖和蔗糖对苯甲腈降解没有影响，但能抑制苯甲酰胺的进一步降解。该研究可为消除环境中苯甲腈及其中间代谢污染物以及探究苯甲腈代谢机制提供理论依据。

关键词: 暗灰链霉菌, 苯甲腈, 苯甲酸, 微生物降解

Abstract: The nitrile-degrading actinomycetes Streptomyces canus CGMCC 13662 was used to study the degradation of benzonitrile and benzoic acid. Growing S. canus cells degraded 0.5 g·L^-1 benzonitrile completely within 120 h, with the final metabolite being benzamide; the resting cells could completely degrade benzonitrile within 24 h and benzamide and benzoic acid were no longer present by the end of the assay. The actinomycetes simultaneously degraded catechol and protocatechuic acid, indicating that the metabolic pathway of benzoic acid was via the catechol and protocatechuic acid pathway. The amount of benzoic acid produced by CoCl₂-induced actinomycetes after 8 h of benzonitrile metabolism was 6-fold lower than the control without cobalt, which indicated that benzonitrile degraded via the NHase/amidase and nitrilase pathways and CoCl₂ could regulate the benzonitrile metabolic flux between them. The degradation rate of benzonitrile decreased under acidic conditions, while benzamide degradation was sensitive to the pH of the resting cell solutions and the maximum benzamide degradation was at pH 7.5. Malate was the optimal co-substrate for benzonitrile degradation, leading to a decrease in the half-life of benzonitrile degradation from 5.40 to 3.80 h compared with degradation without the co-substrate. Glucose and sucrose had no effect on benzonitrile degradation as co-substrates, while they inhibited the further degradation of benzamide. This study provides a theoretical basis for eliminating residues of benzonitrile and intermediate metabolic pollutants in the environment and exploring the metabolic mechanism of benzonitrile.

Key words: Streptomyces canus, benzonitrile, benzoic acid, microbial degradation

中图分类号:

Q939.96

郭铃, 方文婉, 葛峰, 戴亦军. 暗灰链霉菌CGMCC 13662降解苯甲腈和苯甲酸的研究[J]. 生态与农村环境学报, 2020, 36(2): 257-264.

GUO Ling, FANG Wen-wan, GE Feng, DAI Yi-jun. Degradation of Benzonitrile and Benzoic Acid by Actinomycetes Streptomyces canus CGMCC 13662[J]. Journal of Ecology and Rural Environment, 2020, 36(2): 257-264.

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暗灰链霉菌CGMCC 13662降解苯甲腈和苯甲酸的研究

Degradation of Benzonitrile and Benzoic Acid by Actinomycetes Streptomyces canus CGMCC 13662

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