阶梯式降温对半短程硝化效能及菌群结构的影响

doi:10.19741/j.issn.1673-4831.2019.0405

生态与农村环境学报 ›› 2020, Vol. 36 ›› Issue (6): 819-824.doi: 10.19741/j.issn.1673-4831.2019.0405

• 污染控制与修复 • 上一篇

阶梯式降温对半短程硝化效能及菌群结构的影响

王伟^1,2, 李柏林^1,2, 汪月^1,2, 王恒^1,2, 李晔^1,2, 夭开芳^1,2, 黄馨^1,2

1. 武汉理工大学资源与环境工程学院, 湖北武汉 430070;
2. 矿物资源加工与环境湖北省重点实验室, 湖北武汉 430070

收稿日期:2019-05-29 出版日期:2020-06-25 发布日期:2020-06-22
通讯作者: 李柏林 E-mail:bolly1221@whut.edu.cn
作者简介:王伟(1994-),男,安徽合肥人,硕士生,主要从事水处理研究。E-mail:1812216090@qq.com
基金资助:
国家自然科学基金（51708431）；湖北省自然科学基金（2014CFB285）；武汉市应用基础研究计划（2014060101010065）

Effect of Step Cooling on the Efficiency and Functional Flora Structure of a Half Partial Nitrification System

WANG Wei^1,2, LI Bo-lin^1,2, WANG Yue^1,2, WANG Heng^1,2, LI Ye^1,2, YAO Kai-fang^1,2, HUANG Xin^1,2

1. School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China;
2. Hubei Key Laboratory of Mineral Resources Processing and Environment, Wuhan 430070, China

Received:2019-05-29 Online:2020-06-25 Published:2020-06-22

摘要/Abstract

摘要： 接种好氧絮状污泥于SBR反应器中，先快速启动短程硝化，然后进行半短程硝化调控并分析阶梯式降温下功能菌群落结构变化。结果表明，控制ρ（DO）为1.2~1.5 mg·L^-1、pH值为7.6~7.7、温度为（30±2）℃时，在第16天成功启动短程硝化。随后进行半短程硝化调控，使出水NO₂^--N/NH₄⁺-N比值稳定在1.32左右。然后进一步设置阶梯式降温（28 ℃→25 ℃→20 ℃→15 ℃）的实验工况，通过提升ρ（DO）为1.4~1.5 mg·L^-1、进水pH值为7.8~7.9，使反应器在中低温下保持稳定运行，在15 ℃条件下NH₄⁺-N去除率（NRR）均值为59.7%，NO₂^--N积累率（NAR）均值达80.2%。16S rDNA高通量测序检测显示，氨氧化菌（AOB）菌属Nitrosomonas占比由接种污泥中的0.09%上升到15 ℃下的5.20%，菌属丰度提升了58倍左右。亚硝酸盐氧化菌（NOB）菌属Nitrospira和Nitrobacter占比分别由接种污泥的0.90%和0.98%下降到15 ℃下的0.33%和0.05%，AOB丰度明显增加，NOB得到有效抑制。

关键词: SBR反应器, 半短程硝化, 高通量测序, 氨氧化菌, 亚硝酸盐氧化菌

Abstract: Partial nitritation was quickly begun by inoculating aerobic flocculent sludge and half partial nitritation was achieved. The changes in the microbial communities were studied during the process of step cooling. The results show that when controlling ρ(DO) to 1.2-1.5 mg·L^-1, pH to 7.6-7.7, and temperature to (30±2) ℃, partial nitritation could be achieved on the 16th day. Subsequently, half partial nitritation was controlled to stabilize the effluent NO₂^--N/NH₄⁺-N around 1.32. Then a stepwise cooling was set (28 ℃→25 ℃→20 ℃→15 ℃) simultaneously to an increase in ρ(DO) to 1.4-1.5 mg·L^-1 and an increase of pH to 7.8-7.9 to keep the reactor stable at low temperatures. It showed that even at 15 ℃, the NH₄⁺-N removal rate (NRR) and the NO₂^--N accumulation rate (NAR) averaged 59.7% and 80.2%, respectively. According to 16S rDNA high-throughput sequencing tests, the relative abundance of the ammonia oxidizing bacteria (AOB) Nitrosomonas increased from 0.09% in inoculated sludge to 5.20% at 15 ℃, about 58-fold increase. The relative abundance of the nitrite oxidizing bacteria (NOB) Nitrospira and Nitrobacter decreased from 0.90% and 0.98% in the inoculated sludge to 0.33% and 0.05% at 15 ℃, respectively. Thus the number of AOB increased significantly and NOB were effectively inhibited.

Key words: SBR, half shortcut nitrification, high throughput sequencing technology, ammoxidation bacteria, nitrite oxidizing bacteria

中图分类号:

王伟, 李柏林, 汪月, 王恒, 李晔, 夭开芳, 黄馨. 阶梯式降温对半短程硝化效能及菌群结构的影响[J]. 生态与农村环境学报, 2020, 36(6): 819-824.

WANG Wei, LI Bo-lin, WANG Yue, WANG Heng, LI Ye, YAO Kai-fang, HUANG Xin. Effect of Step Cooling on the Efficiency and Functional Flora Structure of a Half Partial Nitrification System[J]. Journal of Ecology and Rural Environment, 2020, 36(6): 819-824.

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阶梯式降温对半短程硝化效能及菌群结构的影响

Effect of Step Cooling on the Efficiency and Functional Flora Structure of a Half Partial Nitrification System

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