工业园区地下水细菌群落结构及影响因素

doi:10.19741/j.issn.1673-4831.2018.0048

生态与农村环境学报 ›› 2019, Vol. 35 ›› Issue (2): 255-263.doi: 10.19741/j.issn.1673-4831.2018.0048

工业园区地下水细菌群落结构及影响因素

许霞¹, 刘菲¹, 薛银刚^1,2, 屠博文³, 周璐璐², 江晓栋¹, 施昕澜², 薛柯¹

1. 常州大学环境与安全工程学院, 江苏常州 213164;
2. 江苏省环境保护水环境生物监测重点实验室/常州市环境监测中心, 江苏常州 213001;
3. 常州市疾病预防控制中心, 江苏常州 213022

收稿日期:2018-01-30 出版日期:2019-02-25 发布日期:2019-03-25
通讯作者: 薛银刚 E-mail:yzxyg@126.com
作者简介:许霞(1982-),女,江苏常州人,讲师,博士,主要从事水污染控制与新技术研究。E-mail:xuxia@cczu.edu.cn
基金资助:
江苏省第十四批"六大人才高峰"项目（JNHB-118）；常州市科技局科技支撑社会发展项目（CE20175022）；国家水体污染控制与治理科技重大专项（2017ZX07202-01）；江苏省自然科学基金青年基金（BK20150250）；江苏省预防医学会科研课题（Y2015016）

Bacterial Community Structure and Its Influence Factors of the Groundwater in an Industry Park

XU Xia¹, LIU Fei¹, XUE Yin-gang^1,2, TU Bo-wen³, ZHOU Lu-lu², JIANG Xiao-dong¹, SHI Xin-lan², XUE Ke¹

1. School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China;
2. Key Laboratory of Environmental Protection of Water Environment Biological Monitoring of Jiangsu Province/Changzhou Environmental Monitoring Center, Changzhou 213001, China;
3. Changzhou Centers for Disease Control and Prevention, Changzhou 213022, China

Received:2018-01-30 Online:2019-02-25 Published:2019-03-25

摘要/Abstract

摘要：

地下水细菌群落结构和多样性是反映其生态系统环境敏感性的重要指标。为研究工业园区地下水细菌群落结构及多样性，于2016年12月对常州市某工业园区地下水进行采集，采用高通量技术对细菌的16S rRNA基因进行序列测定。高通量测序结果表明工业园区地下水细菌群落呈现出典型的淡水种群特征，主要由变形菌门（Proteobacteria，86.40%）、拟杆菌门（Bacteroidetes，5.06%）和厚壁菌门（Firmicutes，3.04%）等55个细菌门，γ-变形菌纲（Gammaproteobacteria，47.51%）、β-变形菌纲（Betaproteobacteria，29.58%）和α-变形菌纲（Alphaproteobacteria，6.75%）等95个细菌纲以及假单胞菌属（Pseudomonas，16.38%），Perlucidibaca（9.17%）和不动杆菌属（Acinetobacter，8.12%）等564个细菌属组成。经分析，水质情况影响着细菌群落组成和多样性，水质较好的点位细菌群落丰富度和均匀度也较高；冗余分析结果表明氯化物、钙、电导率、铁、氟化物和硫酸盐是能显著解释地下水细菌群落的环境因子。

关键词: 工业园区, 地下水, 细菌群落, 高通量测序, 淡水种群

Abstract:

The structure and diversity of bacterial community in groundwater are important indicators to reflect the environmental sensitivity of the ecosystem. In order to investigate the bacterial community structure and diversity of the groundwater in an industrial park, the groundwater samples from an industrial park in Changzhou were collected in December 2016, and the 16S rRNA gene of bacteria was sequenced by high-throughput sequencing. The results show that bacterial community structure of the groundwater present a typical characteristics of freshwater populations and could be divided into 55 phyla, 95 classes and 564 genera. Proteobacteria (86.40%), Bacteroidetes (5.06%) and Firmicutes (3.04%) are the dominant phyla in the studied groundwater. The abundant classes within the phyla Proteobacteria are Gammaproteobacteria (47.51%), Betaproteobacteria (29.58%) and Alphaproteobacteria (6.75%), respectively. At the genus level, Pseudomonas (16.38%), Perlucidibaca (9.17%) and Acinetobacter (8.12%) are predominant. The influence of the physical and chemical indicators on bacterial community show that the water quality affects the composition and diversity of bacterial community, and the better the water quality is, the higher the richness and evenness of bacterial community are. Redundancy analysis results show that chloride, calcium, conductivity, iron, fluoride and sulfate are the environmental factors that can significantly explain the groundwater bacterial community.

Key words: industrial park, groundwater, bacterial community, high-throughput sequencing, freshwater populations

中图分类号:

X832

许霞, 刘菲, 薛银刚, 屠博文, 周璐璐, 江晓栋, 施昕澜, 薛柯. 工业园区地下水细菌群落结构及影响因素[J]. 生态与农村环境学报, 2019, 35(2): 255-263.

XU Xia, LIU Fei, XUE Yin-gang, TU Bo-wen, ZHOU Lu-lu, JIANG Xiao-dong, SHI Xin-lan, XUE Ke. Bacterial Community Structure and Its Influence Factors of the Groundwater in an Industry Park[J]. Journal of Ecology and Rural Environment, 2019, 35(2): 255-263.

参考文献

[1] 孙才志,奚旭,董璐.基于ArcGIS的下辽河平原地下水脆弱性评价及空间结构分析[J].生态学报,2015,35(20):6635-6646.[SUN Cai-zhi,XI Xu,DONG Lu.An ArcGIS-Based Analysis of Groundwater Spatial Structure and Groundwater Vulnerability in the Lower Reaches of the Liaohe River Plain[J].Acta Ecologica Sinica,2015,35(20):6635-6646.]
[2] CAO G L,ZHENG C M,SCANLON B R,et al.Use of Flow Modeling to Assess Sustainability of Groundwater Resources in the North China Plain[J].Water Resources Research,2015,49(1):159-175.
[3] JI Y F,DONG C Y,KONG D Y,et al.Heat-Activated Persulfate Oxidation of Atrazine:Implications for Remediation of Groundwater Contaminated by Herbicides[J].Chemical Engineering Journal,2015,263:45-54.
[4] POSTIGO C,BARCELÓ D.Synthetic Organic Compounds and Their Transformation Products in Groundwater:Occurrence,Fate and Mitigation[J].Science of the Total Environment,2015,503/504:32-47.
[5] WINTER T C.Recent Advances in Understanding the Interaction of Groundwater and Surface Water[J].Reviews of Geophysics,2015,33(Suppl. 1):985-994.
[6] MAXWELL R M,CONDON L E,KOLLET S J.A High-Resolution Simulation of Groundwater and Surface Water Over Most of the Continental US With the Integrated Hydrologic Model ParFlow v3[J].Geoscientific Model Development,2015,8(3):923-937.
[7] 安新丽,陈廷廷,赵晗,等.基于微生物生物完整性指数的地下水生态系统健康评价:以包钢稀土尾矿库周边地下水生态系统为例[J].环境科学,2016,37(9):3413-3422.[AN Xin-li,CHEN Ting-ting,ZHAO Han,et al.Assessment of Ecosystem Health of Baogang Tailings Groundwater Based on Microbiome Index of Biotic Integrity(M-IBI)[J].Environmental Science,2016,37(9):3413-3422.]
[8] 吕保义,谢建云,郑喻,等.稀土尾矿库周边地下水微生物的群落多样性研究[J].环境工程,2015,33(增刊1):101-104,116.[LÜ Bao-yi,XIE Jian-yun,ZHENG Yu,et al.Study on Microbial Biodiverdity in the Groundwater Around Tallings of Rare Earth[J].Environmental Engineering,2015(Suppl. 1):101-104,116.]
[9] LI P,WANG Y H,DAI X Y,et al.Microbial Community in High Arsenic Shallow Groundwater Aquifers in Hetao Basin of Inner Mongolia,China[J].PLoS One,2015,10(5):e0125844.
[10] 郝春博,王广才,董健楠,等.石油污染地下水中细菌多样性研究[J].环境科学,2009,30(8):2464-2472.[HAO Chun-bo,WANG Guang-cai,DONG Jian-nan,et al.Bacterial Biodiversity in the Groundwater Contaminated by Oil[J].Environmental Science,2009,30(8):2464-2472.]
[11] PRONK M,GOLDSCHEIDER N,ZOPFI J.Microbial Communities in Karst Groundwater and Their Potential Use for Biomonitoring[J].Hydrogeology Journal,2009,17(1):37-48.
[12] HEMME C L,DENG Y,GENTRY T J,et al.Metagenomic Insights Into Evolution of a Heavy Metal-Contaminated Groundwater Microbial Community[J].The ISME Journal,2010,4(5):660-672.
[13] LIU B H,LI Y H,MA J F,et al.Detection of Semi-Volatile Organic Compounds(SVOCs) in Surface Water,Soil,and Groundwater in a Chemical Industrial Park in Eastern China[J].Water Science & Technology,2015,73(5):1175-1189.
[14] CHAO Y Q,MAO Y P,WANG Z P,et al.Diversity and Functions of Bacterial Community in Drinking Water Biofilms Revealed by High-Throughput Sequencing[J].Scientific Reports,2015,5:10044,doi:10.1038/srep10044.
[15] PENTON C R,GUPTA V V S R,YU J L,et al.Size Matters:Assessing Optimum Soil Sample Size for Fungal and Bacterial Community Structure Analyses Using High Throughput Sequencing of rRNA Gene Amplicons[J].Frontiers in Microbiology,2016,7:824,doi:10.3389/fmicb.2016.00824.
[16] 薛银刚,江晓栋,孙萌,等.基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究[J].生态与农村环境学报,2017,33(11):992-1000.[XUE Yin-gang,JIANG Xiao-dong,SUN Meng,et al.Structure and Diversity Profiles of Planktonic and Sediment Bacteria Communities in the Zhushan Bay of Lake Taihu in Winter Based on High-Throughput Sequencing[J].Journal of Ecology and Rural Environment,2017,33(11):992-1000.]
[17] GB/T 14848-2017,地下水质量标准[S].[GB/T 14848-2017,Standard for Groundwater Quality[S].]
[18] GB/T 5750-1985,生活饮用水标准检验法[S].[GB/T 5750-1985,Standard Examination Methods for Drinking Water[S].]
[19] QUAST C,PRUESSE E,YILMAZ P,et al.The SILVA Ribosomal RNA Gene Database Project:Improved Data Processing and Web-Based Tools[J].Nucleic Acids Research,2012,41(Database Issue):D590-D596.
[20] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al.QⅡME Allows Analysis of High-Throughput Community Sequencing Data[J].Nature Methods,2010,7(5):335-336.
[21] TER-BRAAK C J F,ŠMILAUER P.CANOCO Reference Manual and CanoDraw for Windows User's Guide:Software for Canonical Community Ordination(Version 4.5)[R].New York,USA:Microcomputer Power,2002.
[22] 生静雅,朱海军,刘广勤,等.不同薄壳山核桃品种的根际土壤细菌菌群结构及多样性研究[J].生态与农村环境学报,2017,33(9):816-821.[SHENG Jing-yu,ZHU Hai-jun,LIU Guang-qin,et al.Community Structure and Diversity of Soil Bacteria in Rhizospheres Under Different Varieties of Carya illinoensis[J].Journal of Ecology and Rural Environment,2017,33(9):816-821.]
[23] GIHRING T M,GREEN S J,SCHADT C W.Massively Parallel rRNA Gene Sequencing Exacerbates the Potential for Biased Community Diversity Comparisons Due to Variable Library Sizes[J].Environmental Microbiology,2011,14(2):285-290.
[24] HUSE S M,WELCH D M,MORRISON H G,et al.Ironing out the Wrinkles in the Rare Biosphere Through Improved OTU Clustering[J].Environmental Microbiology,2010,12(7):1889-1898.
[25] JIA F,LAI C,CHEN L,et al.Spatiotemporal and Species Variations in Prokaryotic Communities Associated With Sediments From Surface-Flow Constructed Wetlands for Treating Swine Wastewater[J].Chemosphere,2017,185:1-10.
[26] 薛禹群,张幼宽.地下水污染防治在我国水体污染控制与治理中的双重意义[J].环境科学学报,2009,29(3):474-481.[XUE Yu-qun,ZHANG Yun-kuan.Two Fold Significance of Groundwater Pollution Prevention in China's Water Pollution Control[J].Acta Scientiae Circumstantiae,2009,29(3):474-481.]
[27] MADSEN E L.Microorganisms and Their Roles in Fundamental Biogeochemical Cycles[J].Current Opinion in Biotechnology,2011,22(3):456-464.
[28] FAN L M,BARRY K,HU G D,et al.Bacterioplankton Community Analysis in Tilapia Ponds by Illumina High-Throughput Sequencing[J].World Journal of Microbiology and Biotechnology,2016,32(1):1-11.
[29] FAN L M,SONG C,MENG S L,et al.Spatial Distribution of Planktonic Bacterial and Archaeal Communities in the Upper Section of the Tidal Reach in Yangtze River[J].Scientific Reports,2016,6:39147.
[30] LI J F,ZHANG J Y,LIU L Y,et al.Annual Periodicity in Planktonic Bacterial and Archaeal Community Composition of Eutrophic Lake Taihu[J].Scientific Reports,2015,5:15488,doi:10.1038/srep15488.
[31] KADNIKOV V V,FRANK Y A,MARDANOV A V,et al.Uncultured Bacteria and Methanogenic Archaea Predominate in the Microbial Community of Western Siberian Deep Subsurface Aquifer[J].Microbiology,2017,86(3):412-415.
[32] BEYER A,RZANNY M,WEIST A,et al.Aquifer Community Structure in Dependence of Lithostratigraphy in Groundwater Reservoirs[J].Environmental Science and Pollution Research,2015,22(24):19342-19351.
[33] BRÜMMER I H M,FELSKE A,WAGNER-DÖBLER I.Diversity and Seasonal Variability of β-Proteobacteria in Biofilms of Polluted Rivers:Analysis by Temperature Gradient Gel Electrophoresis and Cloning[J].Applied and Environmental Microbiology,2003,69(8):4463-4473.
[34] 白洁,刘小沙,侯瑞,等.南海南部海域浮游细菌群落特征及影响因素研究[J].中国环境科学,2014,34(11):2950-2957.[BAI Jie,LIU Xiao-sha,HOU Rui,et al.Community Structure and Influencing Factors of Bacterioplankton in the Southern South China Sea[J].China Environmental Science,2014,34(11):2950-2957.]
[35] SULTAN N S,RAIPAT B S,SINHA M P.Genetic Identification and Characterization of Soil Bacteria Using 16S rDNA Gene Sequence Analysis[J].International Journal of Advanced Research,2013,1(4):155-162.
[36] LIM K T,SHUKOR M Y,WASOH H.Physical,Chemical,and Biological Methods for the Removal of Arsenic Compounds[J].BioMed Research International,2014,2014:503784.
[37] RAWLINGS D E.The Molecular Genetics of Thiobacillus ferrooxidans,and Other Mesophilic,Acidophilic,Chemolithotrophic,Iron-or Sulfur-Oxidizing Bacteria[J].Hydrometallurgy,2001,59(2/3):187-201.
[38] 丁轶睿,李定龙,张毅敏,等.滆湖底泥细菌群落结构及多样性[J].环境科学学报,2017,37(5):1649-1656.[DING Yi-rui,LI Ding-long,ZHANG Yi-min,et al.The Structure and the Diversity of Sediment Microbial Communities in Gehu Lake[J].Acta Scientiae Circumstantiae,2017,37(5):1649-1656]
[39] HOVEN R N V,SANTINI J M.Arsenite Oxidation by the Heterotroph Hydrogenophaga sp.str.NT-14:The Arsenite Oxidase and Its Physiological Electron Acceptor[J].Biochimica et Biophysica Acta(BBA):Bioenergetics,2004,1656(2/3):148-155.
[40] AMALFITANO S,BON A D,ZOPPINI A,et al.Groundwater Geochemistry and Microbial Community Structure in the Aquifer Transition From Volcanic to Alluvial Areas[J].Water Research,2014,65:384-394.
[41] WEI G S,LI J,WANG N X,et al.Spatial Abundance and Diversity of Bacterioplankton in a Typical Stream-Forming Ecosystem,Huangqian Reservoir,China[J].Journal of Microbiology and Biotechnolog,2014,24(10):1308-1318.
[42] WANG J,LI Y,WANG P F,et al.Response of Bacterial Community Compositions to Different Sources of Pollutants in Sediments of a Tributary of Taihu Lake,China[J].Environmental Science and Pollution Research,2016,23(14):13886-13894.

工业园区地下水细菌群落结构及影响因素

Bacterial Community Structure and Its Influence Factors of the Groundwater in an Industry Park

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	顾诗云, 杨飞, 张毅敏, 张志伟, 谢科夫, 管祥洋. 淹水胁迫对菖蒲生理特性及其根际细菌群落特征的影响[J]. 生态与农村环境学报, 2020, 36(4): 488-498.
[2]	李雪, 刘述凤, 鄢雨朦, 张润洁, 王天杰, 付保荣. 复合芽孢杆菌对鲫鱼养殖水体水质及细菌群落结构的影响[J]. 生态与农村环境学报, 2020, 36(4): 522-530.
[3]	洪鑫, 韩成, 孔帆, 周丰武, 吴少松, 钟文辉, 刘标. 抗虫-耐除草剂转基因玉米种植对根际土壤细菌和真菌群落的影响[J]. 生态与农村环境学报, 2020, 36(3): 358-366.
[4]	寄博华, 李玮, 常军军, 李璇, 司光正, 何芳, 陈金全. 滇池湖滨湿地不同挺水植物区沉积物细菌群落结构特征[J]. 生态与农村环境学报, 2020, 36(3): 390-398.
[5]	徐雄, 肖培平, 孙艳亭, 李敏, 董应尚, 高尚赞, 刘文信. 鲁西南黄河冲积平原地下水氟碘特征及成因分析[J]. 生态与农村环境学报, 2020, 36(2): 186-192.
[6]	鲁瀚友, 李志威, 胡旭跃. 基于VMOD模型的若尔盖泥炭沼泽地下水数值模拟[J]. 生态与农村环境学报, 2019, 35(4): 442-450.
[7]	王祥, 邓绍坡, 李川, 万金忠. 修复氯代烃污染的生物炭负载纳米零价铁制备条件优化实验[J]. 生态与农村环境学报, 2019, 35(12): 1626-1632.
[8]	李爽, 詹琪, 任建新, 马会强. 堆肥物以及堆肥物与铁屑联用原位修复硝酸盐污染地下水研究[J]. 生态与农村环境学报, 2018, 34(3): 260-266.
[9]	吴文晖, 邹辉, 朱岗辉, 廖岳华, 潘海婷, 肖辰畅, 樊娟, 李璐. 湘中某矿区地下水重金属污染特征及健康风险评估[J]. 生态与农村环境学报, 2018, 34(11): 1027-1033.
[10]	黄骏宇, 蒋亚萍, 陈余道, 程亚平. 利用米酒为碳源去除农村家庭饮用地下水中硝酸盐的现场试验研究[J]. 生态与农村环境学报, 2018, 34(10): 955-960.
[11]	张逸飞, 徐婷婷, 韩成, 邓欢, 尹力初, 钟文辉. 不同施肥模式和地下水位条件下红壤水稻土PLFA指纹特征[J]. 生态与农村环境学报, 2017, 33(6): 533-538.
[12]	郭敏, 吴文铸, 张圣虎, 宋宁慧, 石利利. 2种甲氧基丙烯酸酯类杀菌剂在土壤中的降解吸附特性及对地下水的影响[J]. 生态与农村环境学报, 2017, 33(5): 460-465.
[13]	薛银刚, 江晓栋, 孙萌, 刘菲, 滕加泉, 耿金菊, 谢文理, 张皓. 基于高通量测序的冬季太湖竺山湾浮游细菌和沉积物细菌群落结构和多样性研究[J]. 生态与农村环境学报, 2017, 33(11): 992-1000.
[14]	王磊, 龙涛, 张峰, 祝欣, 田猛, 林玉锁. 用于土壤及地下水修复的多相抽提技术研究进展[J]. 生态与农村环境学报, 2014, 30(2): 137-145.
[15]	尹艳宁, 方志翔, 沈文静, 刘标. 转cry1Ac+cry2Ab基因棉对土壤细菌群落结构和功能多样性的影响[J]. 生态与农村环境学报, 2014, 30(2): 206-213.